HAMAP logo

HAMAP rule MF_00041

Send feedback

General rule information [?]

Accession MF_00041
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 40)
Name Cys_tRNA_synth
Scope(s) Bacteria
Template(s) P21888 (SYC_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00041 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SYC
Protein name RecName: Full=Cysteine--tRNA ligase;
AltName: Full=Cysteinyl-tRNA synthetase;
Gene name Name=cysS;

Comments [?]

CATALYTIC ACTIVITY Reaction=ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L- cysteinyl-tRNA(Cys); Xref=Rhea:RHEA:17773, Rhea:RHEA-COMP:9661, Rhea:RHEA-COMP:9679, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:35235, ChEBI:CHEBI:78442, ChEBI:CHEBI:78517, ChEBI:CHEBI:456215; EC=;
case <FTGroup:1>
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.;
end case
case <OC:Bacteria>
end case
SIMILARITYBelongs to the class-I aminoacyl-tRNA synthetase family.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0004817; Molecular function:cysteine-tRNA ligase activity
GO:0006423; Biological process:cysteinyl-tRNA aminoacylation
case <FTGroup:1>
GO:0008270; Molecular function:zinc ion binding
end case
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF01406; tRNA-synt_1e; 1;
NCBIfam TIGR00435; CysS; 1;

Features [?]

From: SYC_ECOLI (P21888)
Key From To Description Tag Condition FTGroup
MOTIF 30 40 /note="'HIGH' region" x-T-x(5)-H-[LIVFA]-G-[HN]
MOTIF 266 270 /note="'KMSKS' region" [KR]-M-[GSA]-[KS]-S
BINDING 28 28 /ligand="Zn(2+)"
C 1
BINDING 209 209 /ligand="Zn(2+)"
C 1
BINDING 234 234 /ligand="Zn(2+)"
H 1
BINDING 238 238 /ligand="Zn(2+)"
E 1
BINDING 269 269 /ligand="ATP"
From: SYC_BACSU (Q06752)
Key From To Description Tag Condition FTGroup
case <OC:Bacillaceae>
MOD_RES 270 270 /note="Phosphoserine" S
end case

Additional information [?]

Size range 404-546 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Atypical sequence in STRCO that contains a weird additional N-terminal domain constituted of multiple repeats; not taken into account in size range For archeal missing cysS see: MEDLINE=99412295; PubMed=10482537; Hamann C.S., Sowers K.R., Lipman R.S., Hou Y.M.; "An archaeal aminoacyl-tRNA synthetase missing from genomic analysis."; J. Bacteriol. 181:5880-5884(1999). MEDLINE=20086427; PubMed=10622715; Li T., Graham D.E., Stathopoulos C., Haney P.J., Kim H.S., Vothknecht U., Kitabatake M., Hong K.W., Eggertsson G., Curnow A.W., Lin W., Celic I., Whitman W., Soll D.; "Cysteinyl-tRNA formation: the last puzzle of aminoacyl-tRNA synthesis."; FEBS Lett. 462:302-306(1999).

View rule in raw text format (no links)