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Annotation rule MF_00041 |
Accession | MF_00041 |
Dates | 1-JUN-2001 (Created) 20-NOV-2019 (Last updated, Version 37) |
Name | Cys_tRNA_synth |
Scope | Bacteria
Archaea |
Template | P21888 (SYC_ECOLI) |
Triggered by |
Identifier |
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Protein name |
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Gene name |
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Catalytic activity | RHEA:17773: ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)
EC 6.1.1.16 |
Cofactor | Zn(2+) Note: Binds 1 zinc ion per subunit. |
Subunit | Monomer. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the class-I aminoacyl-tRNA synthetase family. |
Pfam | PF01406; tRNA-synt_1e; 1; |
PRINTS | PR00983; TRNASYNTHCYS; 1; |
TIGRFAMs | TIGR00435; CysS; 1; |
PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
From: SYC_ECOLI (P21888) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
MOTIF | 30 | 40 | 'HIGH' region | x-T-x(5)-H-[LIVFA]-G-[HN] | ||||||||
MOTIF | 266 | 270 | 'KMSKS' region | [KR]-M-[GSA]-[KS]-S | ||||||||
METAL | 28 | 28 | Zinc | C | 1 | |||||||
METAL | 209 | 209 | Zinc | C | 1 | |||||||
METAL | 234 | 234 | Zinc | H | 1 | |||||||
METAL | 238 | 238 | Zinc | E | 1 | |||||||
BINDING | 269 | 269 | ATP | [KS] |
From: SYC_BACSU (Q06752) | ||||||||||||
MOD_RES | 270 | 270 | Phosphoserine | S |
Size range | 404-546 amino acids |
Related rules | None |
Fusion | None |
Comments | Atypical sequence in STRCO that contains a weird additional N-terminal domain constituted of multiple repeats; not taken into account in size range For archeal missing cysS see: MEDLINE=99412295; PubMed=10482537; Hamann C.S., Sowers K.R., Lipman R.S., Hou Y.M.; "An archaeal aminoacyl-tRNA synthetase missing from genomic analysis."; J. Bacteriol. 181:5880-5884(1999). MEDLINE=20086427; PubMed=10622715; Li T., Graham D.E., Stathopoulos C., Haney P.J., Kim H.S., Vothknecht U., Kitabatake M., Hong K.W., Eggertsson G., Curnow A.W., Lin W., Celic I., Whitman W., Soll D.; "Cysteinyl-tRNA formation: the last puzzle of aminoacyl-tRNA synthesis."; FEBS Lett. 462:302-306(1999). |