 |
|
| Annotation rule MF_00041 |
General rule information
[?]
| Accession | MF_00041 |
| Dates | 1-JUN-2001 (Created) 20-NOV-2019 (Last updated, Version 37) |
| Name | Cys_tRNA_synth |
| Scope | Bacteria
Archaea |
| Template | P21888 (SYC_ECOLI) |
| Triggered by |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier |
|
| Protein name |
|
||||||||||||||||
| Gene name |
|
Comments
[?]
| Catalytic activity | RHEA:17773: ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)
EC 6.1.1.16 |
case <FTGroup:1>
| Cofactor | Zn(2+) Note: Binds 1 zinc ion per subunit. |
end case
case <OC:Bacteria>
| Subunit | Monomer. |
end case
| Subcellular location | Cytoplasm. |
| Similarity | Belongs to the class-I aminoacyl-tRNA synthetase family. |
Keywords
[?]
case <FTGroup:1>
end case
case <FT:8>
end case
Gene Ontology
[?]
GO:0005524; Molecular function: ATP binding.
GO:0004817; Molecular function: cysteine-tRNA ligase activity.
GO:0006423; Biological process: cysteinyl-tRNA aminoacylation.
GO:0004817; Molecular function: cysteine-tRNA ligase activity.
GO:0006423; Biological process: cysteinyl-tRNA aminoacylation.
case <FTGroup:1>
GO:0008270; Molecular function: zinc ion binding.
end case
GO:0005737; Cellular component: cytoplasm.
Cross-references
[?]
| Pfam | PF01406; tRNA-synt_1e; 1; |
| PRINTS | PR00983; TRNASYNTHCYS; 1; |
| TIGRFAMs | TIGR00435; CysS; 1; |
| PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
Features
[?]
| From: SYC_ECOLI (P21888) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| MOTIF | 30 | 40 | 'HIGH' region | x-T-x(5)-H-[LIVFA]-G-[HN] | ||||||||
| MOTIF | 266 | 270 | 'KMSKS' region | [KR]-M-[GSA]-[KS]-S | ||||||||
| METAL | 28 | 28 | Zinc | C | 1 | |||||||
| METAL | 209 | 209 | Zinc | C | 1 | |||||||
| METAL | 234 | 234 | Zinc | H | 1 | |||||||
| METAL | 238 | 238 | Zinc | E | 1 | |||||||
| BINDING | 269 | 269 | ATP | [KS] | ||||||||
case <OC:Bacillaceae>
| From: SYC_BACSU (Q06752) | ||||||||||||
| MOD_RES | 270 | 270 | Phosphoserine | S | ||||||||
end case
Additional information
[?]
| Size range | 404-546 amino acids |
| Related rules | None |
| Fusion | None |
| Comments | Atypical sequence in STRCO that contains a weird additional N-terminal domain constituted of multiple repeats; not taken into account in size range For archeal missing cysS see: MEDLINE=99412295; PubMed=10482537; Hamann C.S., Sowers K.R., Lipman R.S., Hou Y.M.; "An archaeal aminoacyl-tRNA synthetase missing from genomic analysis."; J. Bacteriol. 181:5880-5884(1999). MEDLINE=20086427; PubMed=10622715; Li T., Graham D.E., Stathopoulos C., Haney P.J., Kim H.S., Vothknecht U., Kitabatake M., Hong K.W., Eggertsson G., Curnow A.W., Lin W., Celic I., Whitman W., Soll D.; "Cysteinyl-tRNA formation: the last puzzle of aminoacyl-tRNA synthesis."; FEBS Lett. 462:302-306(1999). |