HAMAP rule MF_00041
General rule information
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| Accession | MF_00041 |
| Dates | 1-JUN-2001 (Created)
3-SEP-2024 (Last updated, Version 41) |
| Name | Cys_tRNA_synth |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P21888 (SYC_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00041 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SYC |
| Protein name | RecName: Full=Cysteine--tRNA ligase; EC=6.1.1.16; AltName: Full=Cysteinyl-tRNA synthetase; Short=CysRS; |
| Gene name | Name=cysS; |
Comments
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| CATALYTIC ACTIVITY | Reaction=tRNA(Cys) + L-cysteine + ATP = L-cysteinyl-tRNA(Cys) + AMP + diphosphate; Xref=Rhea:RHEA:17773, Rhea:RHEA-COMP:9661, Rhea:RHEA-COMP:9679, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:35235, ChEBI:CHEBI:78442, ChEBI:CHEBI:78517, ChEBI:CHEBI:456215; EC=6.1.1.16; |
| case <FTGroup:1> | |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.; |
| end case | |
| case <OC:Bacteria> | |
| SUBUNIT | Monomer. |
| end case | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the class-I aminoacyl-tRNA synthetase family. |
Keywords
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| Cytoplasm | |
| Aminoacyl-tRNA synthetase | |
| Protein biosynthesis | |
| Ligase | |
| ATP-binding | |
| Nucleotide-binding | |
| case <FTGroup:1> | |
| Metal-binding | |
| Zinc | |
| end case | |
| case <FT:8> | |
| Phosphoprotein | |
| end case | |
Gene Ontology
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| GO:0005524; Molecular function:ATP binding | |
| GO:0004817; Molecular function:cysteine-tRNA ligase activity | |
| GO:0006423; Biological process:cysteinyl-tRNA aminoacylation | |
| case <FTGroup:1> | |
| GO:0008270; Molecular function:zinc ion binding | |
| end case | |
| GO:0005737; Cellular component:cytoplasm | |
Cross-references
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| Pfam | PF01406; tRNA-synt_1e; 1; |
| PRINTS | PR00983; TRNASYNTHCYS; 1; |
| NCBIfam | TIGR00435; CysS; 1; |
| PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
Features
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| From: SYC_ECOLI (P21888) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| MOTIF | 30 | 40 | /note="'HIGH' region" | x-T-x(5)-H-[LIVFA]-G-[HN] | ||||||||
| MOTIF | 266 | 270 | /note="'KMSKS' region" | [KR]-M-[GSA]-[KS]-S | ||||||||
| BINDING | 28 | 28 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 209 | 209 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 234 | 234 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | 1 | |||||||
| BINDING | 238 | 238 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
E | 1 | |||||||
| BINDING | 269 | 269 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[KS] | ||||||||
| From: SYC_BACSU (Q06752) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Bacillaceae> | ||||||||||||
| MOD_RES | 270 | 270 | /note="Phosphoserine" | S | ||||||||
| end case | ||||||||||||
Additional information
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| Size range | 404-546 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Atypical sequence in STRCO that contains a weird additional N-terminal domain constituted of multiple repeats; not taken into account in size range For archeal missing cysS see: MEDLINE=99412295; PubMed=10482537; Hamann C.S., Sowers K.R., Lipman R.S., Hou Y.M.; "An archaeal aminoacyl-tRNA synthetase missing from genomic analysis."; J. Bacteriol. 181:5880-5884(1999). MEDLINE=20086427; PubMed=10622715; Li T., Graham D.E., Stathopoulos C., Haney P.J., Kim H.S., Vothknecht U., Kitabatake M., Hong K.W., Eggertsson G., Curnow A.W., Lin W., Celic I., Whitman W., Soll D.; "Cysteinyl-tRNA formation: the last puzzle of aminoacyl-tRNA synthesis."; FEBS Lett. 462:302-306(1999). |