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Annotation rule MF_00041
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General rule information [?]

Accession MF_00041
Dates 1-JUN-2001 (Created)
20-NOV-2019 (Last updated, Version 37)
Name Cys_tRNA_synth
Scope
Bacteria
Archaea
Template P21888 (SYC_ECOLI)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
SYC
Protein name
RecName: Full=Cysteine--tRNA ligase;
EC 6.1.1.16;
AltName: Full=Cysteinyl-tRNA synthetase;
Short=CysRS;
Gene name
cysS

Comments [?]

Catalytic activity RHEA:17773: ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)
EC 6.1.1.16
case <FTGroup:1>
Cofactor Zn(2+)
Note: Binds 1 zinc ion per subunit.
end case
case <OC:Bacteria>
Subunit Monomer.
end case
Subcellular location Cytoplasm.
Similarity Belongs to the class-I aminoacyl-tRNA synthetase family.

Keywords [?]

case <FTGroup:1>
end case
case <FT:8>
end case

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004817; Molecular function: cysteine-tRNA ligase activity.
GO:0006423; Biological process: cysteinyl-tRNA aminoacylation.
case <FTGroup:1>
GO:0008270; Molecular function: zinc ion binding.
end case
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF01406; tRNA-synt_1e; 1;
PRINTS PR00983; TRNASYNTHCYS; 1;
TIGRFAMs TIGR00435; CysS; 1;
PROSITE PS00178; AA_TRNA_LIGASE_I; 1;

Features [?]

From: SYC_ECOLI (P21888)
Key     From     To       Description   Tag   Condition   FTGroup
MOTIF     30     40       'HIGH' region     x-T-x(5)-H-[LIVFA]-G-[HN]  
MOTIF     266     270       'KMSKS' region     [KR]-M-[GSA]-[KS]-S  
METAL     28     28       Zinc     C   1
METAL     209     209       Zinc     C   1
METAL     234     234       Zinc     H   1
METAL     238     238       Zinc     E   1
BINDING     269     269       ATP     [KS]  
case <OC:Bacillaceae>
From: SYC_BACSU (Q06752)
MOD_RES     270     270       Phosphoserine     S  
end case

Additional information [?]

Size range 404-546 amino acids
Related rules None
Fusion None
Comments Atypical sequence in STRCO that contains a weird additional N-terminal domain constituted of multiple repeats; not taken into account in size range For archeal missing cysS see: MEDLINE=99412295; PubMed=10482537; Hamann C.S., Sowers K.R., Lipman R.S., Hou Y.M.; "An archaeal aminoacyl-tRNA synthetase missing from genomic analysis."; J. Bacteriol. 181:5880-5884(1999). MEDLINE=20086427; PubMed=10622715; Li T., Graham D.E., Stathopoulos C., Haney P.J., Kim H.S., Vothknecht U., Kitabatake M., Hong K.W., Eggertsson G., Curnow A.W., Lin W., Celic I., Whitman W., Soll D.; "Cysteinyl-tRNA formation: the last puzzle of aminoacyl-tRNA synthesis."; FEBS Lett. 462:302-306(1999).