HAMAP rule MF_00044
General rule information
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Accession | MF_00044 |
Dates | 1-JUN-2001 (Created) 19-NOV-2022 (Last updated, Version 41) |
Name | Asp_tRNA_synth_type1 |
Scope | Bacteria
Archaea |
Templates | P21889 (SYD_ECOLI); P36419 (SYD_THETH); Q5SKD2 (SYD_THET8); P56459 (SYDND_HELPY); Q51422 (SYDND_PSEAE); Q9RUN7 (SYD_DEIRA); O84546 (SYDND_CHLTR): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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case <FTTag:NonDiscr1_bact> or <FTTag:NonDiscr2_bact>
Identifier |
|
Protein name |
|
else
Identifier |
|
Protein name |
|
end case
Gene name |
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Comments
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case <FTTag:NonDiscr1_bact> or <FTTag:NonDiscr2_bact>
Function | Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn). |
Catalytic activity | RHEA:18349: ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx)
EC 6.1.1.23 |
else
Function | Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp). |
Catalytic activity | RHEA:19649: ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp)
EC 6.1.1.12 |
end case
Subunit | Homodimer. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily. |
Keywords
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Gene Ontology
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GO:0004815; Molecular function: aspartate-tRNA ligase activity.
GO:0005524; Molecular function: ATP binding.
GO:0006422; Biological process: aspartyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
GO:0005524; Molecular function: ATP binding.
GO:0006422; Biological process: aspartyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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Pfam | PF02938; GAD; 1; |
PF00152; tRNA-synt_2; 1; | |
PF01336; tRNA_anti-codon; 1; | |
PRINTS | PR01042; TRNASYNTHASP; 1; |
TIGRFAMs | TIGR00459; aspS_bact; 1; |
PROSITE | PS50862; AA_TRNA_LIGASE_II; 1; |
Features
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From: SYD_THETH (P36419) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 223 | 225 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | R-x-E | ||||||||
BINDING | 528 | 531 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | G-x-x-R | ||||||||
REGION | 201 | 204 | Aspartate | Q-x-x-K | ||||||||
BINDING (Optional) | 177 | 177 | /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991 | E | ||||||||
BINDING (Optional) | 223 | 223 | /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991 | R | ||||||||
BINDING (Optional) | 232 | 232 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | Q | ||||||||
BINDING (Optional) | 442 | 442 | /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991 | H | ||||||||
BINDING (Optional) | 476 | 476 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | E | ||||||||
BINDING (Optional) | 483 | 483 | /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991 | R | ||||||||
From: SYDND_PSEAE (Q51422) | ||||||||||||
SITE (Optional) | 31 | 31 | Important for tRNA non-discrimination | NonDiscr1_bact | H | |||||||
SITE (Optional) | 82 | 82 | Important for tRNA non-discrimination | NonDiscr2_bact | G |
Additional information
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Size range | 430-700 amino acids |
Related rules | MF_02075 (SYD) |
Fusion | Nter: <Unknown>; Cter: None |
Comments | This subfamily contains most bacterial aspartate--tRNA ligases and eukaryotic mitochondrial aspartate--tRNA ligases. See MF_02075 for type 2 subfamily. Unknown N-terminal domain in MAGSA. |