HAMAP rule MF_00044

General rule information [?]

Accession	MF_00044
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 42)

Name	Asp_tRNA_synth_type1

Scope

Bacteria

Archaea

Templates

P21889 (SYD_ECOLI); P36419 (SYD_THETH); Q5SKD2 (SYD_THET8); P56459 (SYDND_HELPY); Q51422 (SYDND_PSEAE); Q9RUN7 (SYD_DEIRA); O84546 (SYDND_CHLTR): [Recover all]

Triggered by

HAMAP; MF_00044 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTTag:NonDiscr1_bact> or <FTTag:NonDiscr2_bact>

Identifier

SYDND

Protein name

RecName:		Full=Aspartate--tRNA(Asp/Asn) ligase;
		EC 6.1.1.23;
AltName:		Full=Aspartyl-tRNA synthetase;
		Short=AspRS;
AltName:		Full=Non-discriminating aspartyl-tRNA synthetase;
		Short=ND-AspRS;

else

Identifier

SYD

Protein name

RecName:		Full=Aspartate--tRNA ligase;
		EC 6.1.1.12;
AltName:		Full=Aspartyl-tRNA synthetase;
		Short=AspRS;

end case

Gene name

aspS

Comments [?]

case <FTTag:NonDiscr1_bact> or <FTTag:NonDiscr2_bact>

Function	Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).
Catalytic activity	RHEA:18349: ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx) EC 6.1.1.23

else

Function	Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp).
Catalytic activity	RHEA:19649: ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp) EC 6.1.1.12

end case

Subunit	Homodimer.
Subcellular location	Cytoplasm.
Similarity	Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.

Keywords [?]

Cytoplasm
Aminoacyl-tRNA synthetase
Protein biosynthesis
Ligase
ATP-binding
Nucleotide-binding

Gene Ontology [?]

GO:0004815; Molecular function: aspartate-tRNA ligase activity.
GO:0005524; Molecular function: ATP binding.
GO:0006422; Biological process: aspartyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam	PF02938; GAD; 1;
	PF00152; tRNA-synt_2; 1;
	PF01336; tRNA_anti-codon; 1;
PRINTS	PR01042; TRNASYNTHASP; 1;
NCBIfam	TIGR00459; aspS_bact; 1;
PROSITE	PS50862; AA_TRNA_LIGASE_II; 1;

Features [?]

From: SYD_THETH (P36419)

Key From To Description Tag Condition FTGroup

BINDING 223 225 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 R-x-E

BINDING 528 531 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 G-x-x-R

REGION 201 204 Aspartate Q-x-x-K

BINDING (Optional) 177 177 /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991 E

BINDING (Optional) 223 223 /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991 R

BINDING (Optional) 232 232 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 Q

BINDING (Optional) 442 442 /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991 H

BINDING (Optional) 476 476 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 E

BINDING (Optional) 483 483 /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991 R

From: SYDND_PSEAE (Q51422)

SITE (Optional) 31 31 Important for tRNA non-discrimination NonDiscr1_bact H

SITE (Optional) 82 82 Important for tRNA non-discrimination NonDiscr2_bact G

Additional information [?]

Size range	430-700 amino acids
Related rules	MF_02075 (SYD)
Fusion	Nter: <Unknown>; Cter: None
Comments	This subfamily contains most bacterial aspartate--tRNA ligases and eukaryotic mitochondrial aspartate--tRNA ligases. See MF_02075 for type 2 subfamily. Unknown N-terminal domain in MAGSA.

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