 |
|
Annotation rule MF_00044 |
General rule information
[?]
Accession |
MF_00044 |
Dates |
1-JUN-2001 (Created) 20-NOV-2019 (Last updated, Version 39) |
Name |
Asp_tRNA_synth_type1 |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
case <FTTag:NonDiscr1_bact> or <FTTag:NonDiscr2_bact>
Protein name |
RecName:
|
Full=Aspartate--tRNA(Asp/Asn) ligase;
|
|
EC 6.1.1.23;
|
AltName:
|
Full=Aspartyl-tRNA synthetase;
|
|
Short=AspRS;
|
AltName:
|
Full=Non-discriminating aspartyl-tRNA synthetase;
|
|
Short=ND-AspRS;
|
|
else
Protein name |
RecName:
|
Full=Aspartate--tRNA ligase;
|
|
EC 6.1.1.12;
|
AltName:
|
Full=Aspartyl-tRNA synthetase;
|
|
Short=AspRS;
|
|
end case
case <FTTag:NonDiscr1_bact> or <FTTag:NonDiscr2_bact>
Function |
Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn). |
Catalytic activity |
RHEA:18349: ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx)
EC 6.1.1.23
|
else
Function |
Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp). |
Catalytic activity |
RHEA:19649: ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp)
EC 6.1.1.12
|
end case
Subunit |
Homodimer. |
Subcellular location |
Cytoplasm. |
Similarity |
Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily. |
GO:0004815; Molecular function: aspartate-tRNA ligase activity.
GO:0005524; Molecular function: ATP binding.
GO:0006422; Biological process: aspartyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
From: SYD_THETH (P36419) |
Key
|
|
From
|
|
To
|
|
Description
|
|
Tag
|
|
Condition
|
|
FTGroup
|
NP_BIND
|
|
223
|
|
225
|
|
ATP
|
|
|
|
R-x-E
|
|
|
NP_BIND
|
|
528
|
|
531
|
|
ATP
|
|
|
|
G-x-x-R
|
|
|
REGION
|
|
201
|
|
204
|
|
Aspartate
|
|
|
|
Q-x-x-K
|
|
|
BINDING (Optional)
|
|
177
|
|
177
|
|
Aspartate
|
|
|
|
E
|
|
|
BINDING (Optional)
|
|
223
|
|
223
|
|
Aspartate
|
|
|
|
R
|
|
|
BINDING (Optional)
|
|
232
|
|
232
|
|
ATP
|
|
|
|
Q
|
|
|
BINDING (Optional)
|
|
442
|
|
442
|
|
Aspartate
|
|
|
|
H
|
|
|
BINDING (Optional)
|
|
476
|
|
476
|
|
ATP
|
|
|
|
E
|
|
|
BINDING (Optional)
|
|
483
|
|
483
|
|
Aspartate
|
|
|
|
R
|
|
|
From: SYDND_PSEAE (Q51422) |
SITE (Optional)
|
|
31
|
|
31
|
|
Important for tRNA non-discrimination
|
|
NonDiscr1_bact
|
|
H
|
|
|
SITE (Optional)
|
|
82
|
|
82
|
|
Important for tRNA non-discrimination
|
|
NonDiscr2_bact
|
|
G
|
|
|
Additional information
[?]
Size range |
430-700 amino acids |
Related rules |
MF_02075 (SYD) |
Fusion |
Nter: <Unknown>; Cter: None |
Comments |
This subfamily contains most bacterial aspartate--tRNA ligases and eukaryotic mitochondrial aspartate--tRNA ligases. See MF_02075 for type 2 subfamily. Unknown N-terminal domain in MAGSA. |