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HAMAP rule MF_00060

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_00060
Accession MF_00060
Dates 1-JUN-2001 (Created)
3-SEP-2024 (Last updated, Version 42)
Name SurE
Scope(s) Bacteria
Archaea
Template(s) P96112 (SURE_THEMA); P0A840 (SURE_ECOLI); Q8ZU79 (SURE1_PYRAE); [ Recover all ]
Triggered by HAMAP; MF_00060 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SURE
case <OC:Enterobacterales>
Protein name RecName: Full=5'/3'-nucleotidase SurE;
                 EC=3.1.3.5;
                 EC=3.1.3.6;
AltName: Full=Exopolyphosphatase;
                 EC=3.6.1.11;
AltName: Full=Nucleoside monophosphate phosphohydrolase;
end case
case not <OC:Enterobacterales>
Protein name RecName: Full=5'-nucleotidase SurE;
                 EC=3.1.3.5;
AltName: Full=Nucleoside 5'-monophosphate phosphohydrolase;
end case
Gene name Name=surE;

Comments [?]

case <OC:Enterobacterales>
FUNCTIONNucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5'-monophosphates and ribonucleoside 3'-monophosphates with highest affinity to 3'-AMP. Also hydrolyzes polyphosphate (exopolyphosphatase activity) with the preference for short-chain-length substrates (P20-25). Might be involved in the regulation of dNTP and NTP pools, and in the turnover of 3'-mononucleotides produced by numerous intracellular RNases (T1, T2, and F) during the degradation of various RNAs.
CATALYTIC ACTIVITY Reaction=a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate; Xref=Rhea:RHEA:12484, ChEBI:CHEBI:15377, ChEBI:CHEBI:18254, ChEBI:CHEBI:43474, ChEBI:CHEBI:58043; EC=3.1.3.5;
CATALYTIC ACTIVITY Reaction=a ribonucleoside 3'-phosphate + H2O = a ribonucleoside + phosphate; Xref=Rhea:RHEA:10144, ChEBI:CHEBI:13197, ChEBI:CHEBI:15377, ChEBI:CHEBI:18254, ChEBI:CHEBI:43474; EC=3.1.3.6;
CATALYTIC ACTIVITY Reaction=[phosphate](n) + H2O = [phosphate](n-1) + phosphate + H(+); Xref=Rhea:RHEA:21528, Rhea:RHEA-COMP:9859, Rhea:RHEA-COMP:14279, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16838, ChEBI:CHEBI:43474; EC=3.6.1.11;
end case
case not <OC:Enterobacterales>
FUNCTIONNucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates.
CATALYTIC ACTIVITY Reaction=a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate; Xref=Rhea:RHEA:12484, ChEBI:CHEBI:15377, ChEBI:CHEBI:18254, ChEBI:CHEBI:43474, ChEBI:CHEBI:58043; EC=3.1.3.5;
end case
COFACTOR Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240; Note=Binds 1 divalent metal cation per subunit.;
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the SurE nucleotidase family.

Keywords [?]

Cytoplasm
Hydrolase
Metal-binding
Nucleotide-binding

Gene Ontology [?]

GO:0008253; Molecular function:5'-nucleotidase activity
case <OCellular component:Enterobacterales>
GO:0004309; Molecular function:exopolyphosphatase activity
GO:0008254; Molecular function:3'-nucleotidase activity
end case
GO:0046872; Molecular function:metal ion binding
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF01975; SurE; 1;
NCBIfam TIGR00087; SurE; 1;

Features [?]

From: SURE_THEMA (P96112)
Key From To Description Tag Condition FTGroup
BINDING 8 8 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"		 D
BINDING 9 9 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"		 D
BINDING 39 39 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"		 S
BINDING 95 95 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"		 N

Additional information [?]

Size range 244-283 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments In some organisms, surE was originally annotated as an acid phosphatase (EC 3.1.3.2). Weird C-terminal sequence in TREPA; no obvious frameshift; not shown in alignment and not used in size range. There are divergent second copies of surE in NOSS1 (alr3139) and SYNY3 (sll1459).



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