HAMAP rule MF_00063

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_00063
Accession	MF_00063
Dates	28-FEB-2005 (Created) 03-SEP-2024 (Last updated, Version 25)
Name	CysH
Scope(s)	Bacteria
Template(s)	P17854; O05927; P9WIK3; P56891; P94498; A0R0W2; [ Recover all ]
Triggered by	HAMAP; MF_00063 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	CYSH
case <FTGroup:1>
Protein name	RecName: Full=Adenosine 5'-phosphosulfate reductase; Short=APS reductase; EC=1.8.4.10; AltName: Full=5'-adenylylsulfate reductase; AltName: Full=Thioredoxin-dependent 5'-adenylylsulfate reductase;
else
Protein name	RecName: Full=Phosphoadenosine 5'-phosphosulfate reductase; Short=PAPS reductase; EC=1.8.4.8; AltName: Full=3'-phosphoadenylylsulfate reductase; AltName: Full=PAPS reductase, thioredoxin dependent; AltName: Full=PAPS sulfotransferase; AltName: Full=PAdoPS reductase;
end case
Gene name	Name=cysH;

Comments [?]

case <FTGroup:1>
FUNCTION	Catalyzes the formation of sulfite from adenosine 5'- phosphosulfate (APS) using thioredoxin as an electron donor.
CATALYTIC ACTIVITY	Reaction=[thioredoxin]-disulfide + sulfite + AMP + 2 H(+) = adenosine 5'-phosphosulfate + [thioredoxin]-dithiol; Xref=Rhea:RHEA:21976, Rhea:RHEA-COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:15378, ChEBI:CHEBI:17359, ChEBI:CHEBI:29950, ChEBI:CHEBI:50058, ChEBI:CHEBI:58243, ChEBI:CHEBI:456215; EC=1.8.4.10;
COFACTOR	Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster per subunit.;
PATHWAY	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate.
else
FUNCTION	Catalyzes the formation of sulfite from phosphoadenosine 5'- phosphosulfate (PAPS) using thioredoxin as an electron donor.
CATALYTIC ACTIVITY	Reaction=[thioredoxin]-disulfide + sulfite + adenosine 3',5'- bisphosphate + 2 H(+) = [thioredoxin]-dithiol + 3'-phosphoadenylyl sulfate; Xref=Rhea:RHEA:11724, Rhea:RHEA-COMP:10698, Rhea:RHEA- COMP:10700, ChEBI:CHEBI:15378, ChEBI:CHEBI:17359, ChEBI:CHEBI:29950, ChEBI:CHEBI:50058, ChEBI:CHEBI:58339, ChEBI:CHEBI:58343; EC=1.8.4.8;
PATHWAY	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3.
end case
SUBCELLULAR LOCATION	Cytoplasm.
SIMILARITY	Belongs to the PAPS reductase family. CysH subfamily.

Keywords [?]

Gene Ontology [?]

GO:0004604; Molecular function:phosphoadenylyl-sulfate reductase (thioredoxin) activity
case <FTGroup:1>
GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding
end case
GO:0019379; Biological process:sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)
GO:0070814; Biological process:hydrogen sulfide biosynthetic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Features [?]

From: CYSH_PSEAE (O05927)

Key

From

Description

Tag

Condition

FTGroup

ACT_SITE

256

/note="Nucleophile; cysteine thiosulfonate intermediate"

BINDING

139

/ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"

BINDING

140

/ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"

BINDING

228

/ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"

BINDING

231

/ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"

Additional information [?]

Size range	200-300 amino acids
Related rules	None
Fusion	Nter: None Cter: None
Comments	The presence of an iron-sulfur cluster determines the APS specificity of the sulfate-reducing enzymes and thus separates the APS- and PAPS-dependent assimilatory sulfate reduction pathways. See PubMed:11940598.