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Annotation rule MF_00063
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General rule information [?]

Accession MF_00063
Dates 1-JUN-2001 (Created)
25-FEB-2021 (Last updated, Version 32)
Name CysH
Scope
Bacteria
Templates P17854 (CYSH_ECOLI); O05927 (CYSH_PSEAE); P9WIK3 (CYSH_MYCTU); P56891 (CYSH_RHIME); P94498 (CYSH1_BACSU); A0R0W2 (CYSH_MYCS2): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
CYSH
case <FTGroup:1>
Protein name
RecName: Full=Adenosine 5'-phosphosulfate reductase;
Short=APS reductase;
EC 1.8.4.10;
AltName: Full=5'-adenylylsulfate reductase;
AltName: Full=Thioredoxin-dependent 5'-adenylylsulfate reductase;
else
Protein name
RecName: Full=Phosphoadenosine 5'-phosphosulfate reductase;
Short=PAPS reductase;
EC 1.8.4.8;
AltName: Full=3'-phosphoadenylylsulfate reductase;
AltName: Full=PAPS reductase, thioredoxin dependent;
AltName: Full=PAPS sulfotransferase;
AltName: Full=PAdoPS reductase;
end case
Gene name
cysH

Comments [?]

case <FTGroup:1>
Function Catalyzes the formation of sulfite from adenosine 5'-phosphosulfate (APS) using thioredoxin as an electron donor.
Catalytic activity RHEA:21976: [thioredoxin]-disulfide + AMP + 2 H(+) + sulfite = [thioredoxin]-dithiol + adenosine 5'-phosphosulfate
EC 1.8.4.10
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster per subunit.
Pathway Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate.
else
Function Catalyzes the formation of sulfite from phosphoadenosine 5'-phosphosulfate (PAPS) using thioredoxin as an electron donor.
Catalytic activity RHEA:11724: [thioredoxin]-disulfide + adenosine 3',5'-bisphosphate + 2 H(+) + sulfite = 3'-phosphoadenylyl sulfate + [thioredoxin]-dithiol
EC 1.8.4.8
Pathway Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3.
end case
Subcellular location Cytoplasm.
Similarity Belongs to the PAPS reductase family. CysH subfamily.

Keywords [?]

Cytoplasm
Oxidoreductase
case <FTGroup:1>
Iron
Iron-sulfur
Metal-binding
end case

Gene Ontology [?]

GO:0004604; Molecular function: phosphoadenylyl-sulfate reductase (thioredoxin) activity.
case <FTGroup:1>
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
end case
GO:0019379; Biological process: sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin).
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF01507; PAPS_reduct; 1;
PIRSF PIRSF000857; PAPS_reductase; 1;
TIGRFAMs TIGR00434; cysH; 1;
TIGR02055; APS_reductase; 0-1;
TIGR02057; PAPS_reductase; 0-1;

Features [?]

From: CYSH_PSEAE (O05927)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     256     256       Nucleophile; cysteine thiosulfonate intermediate     C  
METAL     139     139       Iron-sulfur (4Fe-4S)     C   1
METAL     140     140       Iron-sulfur (4Fe-4S)     C   1
METAL     228     228       Iron-sulfur (4Fe-4S)     C   1
METAL     231     231       Iron-sulfur (4Fe-4S)     C   1

Additional information [?]

Size range 200-300 amino acids
Related rules None
Fusion None
Comments The presence of an iron-sulfur cluster determines the APS specificity of the sulfate-reducing enzymes and thus separates the APS- and PAPS-dependent assimilatory sulfate reduction pathways. See PubMed:11940598.


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