HAMAP rule MF_00069

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_00069
Accession	MF_00069
Dates	28-FEB-2005 (Created) 03-SEP-2024 (Last updated, Version 35)
Name	Hydroxylam_reduct
Scope(s)	Bacteria Archaea
Template(s)	P31101; Q01770; P75825; Q6WRT6; [ Recover all ]
Triggered by	HAMAP; MF_00069 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	HCP
Protein name	RecName: Full=Hydroxylamine reductase; EC=1.7.99.1; AltName: Full=Hybrid-cluster protein; Short=HCP; AltName: Full=Prismane protein;
Gene name	Name=hcp;

Comments [?]

FUNCTION	Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O.
CATALYTIC ACTIVITY	Reaction=A + NH4(+) + H2O = hydroxylamine + AH2 + H(+); Xref=Rhea:RHEA:22052, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15429, ChEBI:CHEBI:17499, ChEBI:CHEBI:28938; EC=1.7.99.1;
case <OC:Gammaproteobacteria> and <FTGroup:1>
COFACTOR	Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; Note=Binds 1 [2Fe-2S] cluster.;
end case
case not <OC:Gammaproteobacteria> and <FTGroup:2>
COFACTOR	Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster.;
end case
case <FTGroup:3>
COFACTOR	Name=hybrid [4Fe-2O-2S] cluster; Xref=ChEBI:CHEBI:60519; Note=Binds 1 hybrid [4Fe-2O-2S] cluster.;
end case
SUBCELLULAR LOCATION	Cytoplasm.
SIMILARITY	Belongs to the HCP family.

Keywords [?]

Cytoplasm
Oxidoreductase
case <FTGroup:1> or <FTGroup:2> or <FTGroup:3>
Metal-binding
Iron
Iron-sulfur
end case
case <OC:Gammaproteobacteria> and <FTGroup:1>
2Fe-2S
end case
case not <OC:Gammaproteobacteria> and <FTGroup:2>
4Fe-4S
end case

Gene Ontology [?]

GO:0005737; Cellular component:cytoplasm

GO:0050418; Molecular function:hydroxylamine reductase activity

GO:0051536; Molecular function:iron-sulfur cluster binding

Cross-references [?]

Pfam	PF03063; Prismane; 1;
NCBIfam	TIGR01703; hybrid_clust; 1;

Features [?]

From: HCP_ECOLI (P75825)
Key	From	To	Description	Tag	Condition	FTGroup
case <OC:Gammaproteobacteria>
BINDING	3	3	/ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135"		C	1
BINDING	6	6	/ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135"		C	1
BINDING	18	18	/ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135"		C	1
BINDING	25	25	/ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135"		C	1
end case
From: HCP_NITV2 (P31101)
Key	From	To	Description	Tag	Condition	FTGroup
case not <OC:Gammaproteobacteria>
BINDING	3	3	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883"		C	2
BINDING	6	6	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883"		C	2
BINDING	15	15	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883"		C	2
BINDING	21	21	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883"		C	2
end case
BINDING	244	244	/ligand="hybrid [4Fe-2O-2S] cluster" /ligand_id="ChEBI:CHEBI:60519"		H	3
BINDING	268	268	/ligand="hybrid [4Fe-2O-2S] cluster" /ligand_id="ChEBI:CHEBI:60519"		E	3
BINDING	312	312	/ligand="hybrid [4Fe-2O-2S] cluster" /ligand_id="ChEBI:CHEBI:60519"		C	3
BINDING	406	406	/ligand="hybrid [4Fe-2O-2S] cluster" /ligand_id="ChEBI:CHEBI:60519" /note="via persulfide group"		C	3
BINDING	434	434	/ligand="hybrid [4Fe-2O-2S] cluster" /ligand_id="ChEBI:CHEBI:60519"		C	3
BINDING	459	459	/ligand="hybrid [4Fe-2O-2S] cluster" /ligand_id="ChEBI:CHEBI:60519"		C	3
BINDING	494	494	/ligand="hybrid [4Fe-2O-2S] cluster" /ligand_id="ChEBI:CHEBI:60519"		E	3
BINDING	496	496	/ligand="hybrid [4Fe-2O-2S] cluster" /ligand_id="ChEBI:CHEBI:60519"		K	3
MOD_RES	406	406	/note="Cysteine persulfide"		C

Additional information [?]

Size range	427-570 amino acids
Related rules	None
Fusion	Nter: None Cter: None
Comments	Possible wrong start in THEMA and ACIFR. Deleted domain in THEMA/METTH.