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Annotation rule MF_00087
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General rule information [?]

Accession MF_00087
Dates 1-JUN-2001 (Created)
20-NOV-2019 (Last updated, Version 46)
Name Glu_tRNA_reductase
Templates P0A6X1 (HEM1_ECOLI); Q9UXR8 (HEM1_METKA); P28462 (HEM1_CHLP8); P42809 (HEM1_METTM); P28463 (HEM1_SYNY3); Q59292 (HEMA_RUMJO); P42808 (HEM1_XANCH): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Glutamyl-tRNA reductase;
Gene name

Comments [?]

Function Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).
Catalytic activity RHEA:12344: (S)-4-amino-5-oxopentanoate + NADP(+) + tRNA(Glu) = H(+) + L-glutamyl-tRNA(Glu) + NADPH
Pathway Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.
case <Property:PHOTOSYN>
Pathway Porphyrin-containing compound metabolism; chlorophyll biosynthesis.
end case
Subunit Homodimer.
case <OG:Chloroplast>
Subcellular location Plastid, chloroplast.
end case
Domain Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.
Miscellaneous During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.
Similarity Belongs to the glutamyl-tRNA reductase family.

Keywords [?]

case <Property:PHOTOSYN>
end case

Gene Ontology [?]

GO:0008883; Molecular function: glutamyl-tRNA reductase activity.
case <Property:PHOTOSYN>
GO:0015995; Biological process: chlorophyll biosynthetic process.
end case
GO:0006779; Biological process: porphyrin-containing compound biosynthetic process.
case <OG:Chloroplast>
GO:0009507; Cellular component: chloroplast.
end case

Cross-references [?]

Pfam PF01488; Shikimate_DH; 1;
TIGRFAMs TIGR01035; HemA; 1;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     174     179       NADP        
REGION     47     50       Substrate binding     T-C-x-R  
REGION     99     101       Substrate binding     [ED]-x-[EDQ]  
ACT_SITE     48     48       Nucleophile     C  
BINDING     94     94       Substrate     S  
BINDING     105     105       Substrate     Q  
SITE (Optional)     84     84       Important for activity     H  

Additional information [?]

Size range 329-497 amino acids
Related rules None
Fusion None
Comments STRAW and STRCO have an internal inserted domains of about 120 residues; sequence not included in alignment. Highly divergent RUMJO; sequence not included in alignment SORC5 has a long C-terminus; not included in alignment