HAMAP rule MF_00089
General rule information
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| Accession | MF_00089 |
| Dates | 1-JUN-2001 (Created)
3-SEP-2024 (Last updated, Version 42) |
| Name | ThiC |
| Scope(s) |
Bacteria Archaea |
| Template(s) | Q9A6Q5 (THIC_CAUVC); Q9L9I7 (THIC_SALTY); P30136 (THIC_ECOLI); P45740 (THIC_BACSU); [ Recover all ] |
| Triggered by |
HAMAP; MF_00089 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | THIC |
| Protein name | RecName: Full=Phosphomethylpyrimidine synthase; EC=4.1.99.17; AltName: Full=Hydroxymethylpyrimidine phosphate synthase; Short=HMP-P synthase; Short=HMP-phosphate synthase; Short=HMPP synthase; AltName: Full=Thiamine biosynthesis protein ThiC; |
| Gene name | Name=thiC; |
Comments
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| FUNCTION | Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction. |
| CATALYTIC ACTIVITY | Reaction=5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + S-adenosyl-L- methionine = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + CO + 5'-deoxyadenosine + formate + L-methionine + 3 H(+); Xref=Rhea:RHEA:24840, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740, ChEBI:CHEBI:17245, ChEBI:CHEBI:17319, ChEBI:CHEBI:57844, ChEBI:CHEBI:58354, ChEBI:CHEBI:59789, ChEBI:CHEBI:137981; EC=4.1.99.17; |
| case <FTGroup:2> | |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.; |
| end case | |
| PATHWAY | Cofactor biosynthesis; thiamine diphosphate biosynthesis. |
| case <OC:Pseudomonadota> | |
| SUBUNIT | Homodimer. |
| end case | |
| SIMILARITY | Belongs to the ThiC family. |
Keywords
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| case <FTGroup:2> | |
| 4Fe-4S | |
| Iron | |
| Iron-sulfur | |
| end case | |
| Lyase | |
| Metal-binding | |
| S-adenosyl-L-methionine | |
| Thiamine biosynthesis | |
| case <FTGroup:1> | |
| Zinc | |
| end case | |
Gene Ontology
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| case <FTGroup:1> | |
| GO:0008270; Molecular function:zinc ion binding | |
| end case | |
| GO:0016829; Molecular function:lyase activity | |
| case <FTGroup:2> | |
| GO:0051536; Molecular function:iron-sulfur cluster binding | |
| end case | |
| GO:0009229; Biological process:thiamine diphosphate biosynthetic process | |
Cross-references
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Features
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| From: THIC_CAUVC (Q9A6Q5) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 333 | 335 | /ligand="substrate" | S-[RKY]-G | ||||||||
| BINDING | 374 | 377 | /ligand="substrate" | [DN]-x-x-R | ||||||||
| BINDING | 417 | 417 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | 1 | |||||||
| BINDING | 481 | 481 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | 1 | |||||||
| BINDING | 561 | 561 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | 2 | |||||||
| BINDING | 564 | 564 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | 2 | |||||||
| BINDING | 569 | 569 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | 2 | |||||||
| BINDING | 219 | 219 | /ligand="substrate" | N | ||||||||
| BINDING | 248 | 248 | /ligand="substrate" | M | ||||||||
| BINDING | 277 | 277 | /ligand="substrate" | Y | ||||||||
| BINDING | 313 | 313 | /ligand="substrate" | H | ||||||||
| BINDING | 413 | 413 | /ligand="substrate" | E | ||||||||
| BINDING | 440 | 440 | /ligand="substrate" | [YF] | ||||||||
Additional information
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| Size range | 424-721 amino acids |
| Related rules |
None |
| Fusion | Nter: MF_00097 (thiE) Cter: None |
| Comments | Possible N-terminal domain in some bacterial ThiC. Fused with ThiE (thiamine-phosphate pyrophosphorylase, MF_00097) in BIFLO. |