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HAMAP rule MF_00089

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General rule information [?]

Accession MF_00089
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 40)
Name ThiC
Templates Q9A6Q5 (THIC_CAUVC); Q9L9I7 (THIC_SALTY); P30136 (THIC_ECOLI); P45740 (THIC_BACSU): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Phosphomethylpyrimidine synthase;
AltName: Full=Hydroxymethylpyrimidine phosphate synthase;
Short=HMP-P synthase;
Short=HMP-phosphate synthase;
Short=HMPP synthase;
AltName: Full=Thiamine biosynthesis protein ThiC;
Gene name

Comments [?]

Function Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.
Catalytic activity RHEA:24840: 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + CO + formate + 3 H(+) + L-methionine
case <FTGroup:2>
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
end case
Pathway Cofactor biosynthesis; thiamine diphosphate biosynthesis.
case <OC:Pseudomonadota>
Subunit Homodimer.
end case
Similarity Belongs to the ThiC family.

Keywords [?]

case <FTGroup:2>
end case
case <FTGroup:1>
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0008270; Molecular function: zinc ion binding.
end case
GO:0016829; Molecular function: lyase activity.
case <FTGroup:2>
GO:0051536; Molecular function: iron-sulfur cluster binding.
end case
GO:0009228; Biological process: thiamine biosynthetic process.

Cross-references [?]

Pfam PF01964; ThiC; 1;
NCBIfam TIGR00190; ThiC; 1;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
BINDING     333     335       /ligand="substrate     S-[RKY]-G  
BINDING     374     377       /ligand="substrate     [DN]-x-x-R  
BINDING     417     417       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     H   1
BINDING     481     481       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     H   1
BINDING     561     561       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet     C   2
BINDING     564     564       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet     C   2
BINDING     569     569       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet     C   2
BINDING (Optional)     219     219       /ligand="substrate     N  
BINDING     248     248       /ligand="substrate     M  
BINDING     277     277       /ligand="substrate     Y  
BINDING     313     313       /ligand="substrate     H  
BINDING     413     413       /ligand="substrate     E  
BINDING     440     440       /ligand="substrate     [YF]  

Additional information [?]

Size range 424-721 amino acids
Related rules None
Fusion Nter: MF_00097 (thiE); Cter: None
Comments Possible N-terminal domain in some bacterial ThiC. Fused with ThiE (thiamine-phosphate pyrophosphorylase, MF_00097) in BIFLO.