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HAMAP rule MF_00089

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General rule information [?]

Accession MF_00089
Dates 1-JUN-2001 (Created)
1-MAR-2024 (Last updated, Version 41)
Name ThiC
Scope(s) Bacteria
Archaea
Template(s) Q9A6Q5 (THIC_CAUVC); Q9L9I7 (THIC_SALTY); P30136 (THIC_ECOLI); P45740 (THIC_BACSU); [ Recover all ]
Triggered by HAMAP; MF_00089 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier THIC
Protein name RecName: Full=Phosphomethylpyrimidine synthase;
                 EC=4.1.99.17;
AltName: Full=Hydroxymethylpyrimidine phosphate synthase;
                 Short=HMP-P synthase;
                 Short=HMP-phosphate synthase;
                 Short=HMPP synthase;
AltName: Full=Thiamine biosynthesis protein ThiC;
Gene name Name=thiC;

Comments [?]

FUNCTIONCatalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.
CATALYTIC ACTIVITY Reaction=5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + S-adenosyl-L- methionine = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'- deoxyadenosine + CO + formate + 3 H(+) + L-methionine; Xref=Rhea:RHEA:24840, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740, ChEBI:CHEBI:17245, ChEBI:CHEBI:17319, ChEBI:CHEBI:57844, ChEBI:CHEBI:58354, ChEBI:CHEBI:59789, ChEBI:CHEBI:137981; EC=4.1.99.17;
case <FTGroup:2>
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.;
end case
PATHWAYCofactor biosynthesis; thiamine diphosphate biosynthesis.
case <OC:Pseudomonadota>
SUBUNITHomodimer.
end case
SIMILARITYBelongs to the ThiC family.

Keywords [?]

case <FTGroup:2>
4Fe-4S
Iron
Iron-sulfur
end case
Lyase
Metal-binding
S-adenosyl-L-methionine
Thiamine biosynthesis
case <FTGroup:1>
Zinc
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0008270; Molecular function:zinc ion binding
end case
GO:0016829; Molecular function:lyase activity
case <FTGroup:2>
GO:0051536; Molecular function:iron-sulfur cluster binding
end case
GO:0009229; Biological process:thiamine diphosphate biosynthetic process

Cross-references [?]

Pfam PF01964; ThiC; 1;
NCBIfam TIGR00190; ThiC; 1;

Features [?]

From: THIC_CAUVC (Q9A6Q5)
Key From To Description Tag Condition FTGroup
BINDING 333 335 /ligand="substrate" S-[RKY]-G
BINDING 374 377 /ligand="substrate" [DN]-x-x-R
BINDING 417 417 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 H 1
BINDING 481 481 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 H 1
BINDING 561 561 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_note="4Fe-4S-S-AdoMet"		 C 2
BINDING 564 564 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_note="4Fe-4S-S-AdoMet"		 C 2
BINDING 569 569 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_note="4Fe-4S-S-AdoMet"		 C 2
BINDING 219 219 /ligand="substrate" N
BINDING 248 248 /ligand="substrate" M
BINDING 277 277 /ligand="substrate" Y
BINDING 313 313 /ligand="substrate" H
BINDING 413 413 /ligand="substrate" E
BINDING 440 440 /ligand="substrate" [YF]

Additional information [?]

Size range 424-721 amino acids
Related rules None
Fusion Nter: MF_00097 (thiE) Cter: None
Comments Possible N-terminal domain in some bacterial ThiC. Fused with ThiE (thiamine-phosphate pyrophosphorylase, MF_00097) in BIFLO.



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