HAMAP rule MF_00098
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00098 |
| Accession | MF_00098 |
| Dates | 29-OCT-2002 (Created)
3-SEP-2024 (Last updated, Version 43) |
| Name | Met_tRNA_synth_type1 |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P00959 (SYM_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00098 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SYM |
| Protein name | RecName: Full=Methionine--tRNA ligase; EC=6.1.1.10; AltName: Full=Methionyl-tRNA synthetase; Short=MetRS; |
| Gene name | Name=metG; |
Comments
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| FUNCTION | Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation. |
| CATALYTIC ACTIVITY | Reaction=tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate; Xref=Rhea:RHEA:13481, Rhea:RHEA-COMP:9667, Rhea:RHEA-COMP:9698, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57844, ChEBI:CHEBI:78442, ChEBI:CHEBI:78530, ChEBI:CHEBI:456215; EC=6.1.1.10; |
| case <FTGroup:1> | |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.; |
| end case | |
| case <Feature:PS50886> | |
| SUBUNIT | Homodimer. |
| end case | |
| case not <Feature:PS50886> and <OC:Bacteria> | |
| SUBUNIT | Monomer. |
| end case | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. |
Keywords
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| Cytoplasm | |
| Aminoacyl-tRNA synthetase | |
| Protein biosynthesis | |
| Ligase | |
| ATP-binding | |
| Nucleotide-binding | |
| case <Feature:PS50886> | |
| RNA-binding | |
| tRNA-binding | |
| end case | |
| case <FTGroup:1> | |
| Metal-binding | |
| Zinc | |
| end case | |
Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0004825; Molecular function:methionine-tRNA ligase activity |
| GO:0006431; Biological process:methionyl-tRNA aminoacylation |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF00133; tRNA-synt_1; 1; |
| PRINTS | PR01041; TRNASYNTHMET; 1; |
| NCBIfam | TIGR00398; MetG; 1; |
| NCBIfam | TIGR00399; MetG_C_term; 1; |
| PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
| PROSITE | PS50886; TRBD; 0-1; |
Features
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| From: SYM_ECOLI (P00959) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| MOTIF | 15 | 25 | /note="'HIGH' region" | [PYA]-[YT]-x(5)-H-[LIVFA]-G-[HNST] | ||||||||
| MOTIF | 333 | 337 | /note="'KMSKS' region" | [KTQ]-[LIMF]-[SG]-[KTS]-[ST] | ||||||||
| BINDING | 146 | 146 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 149 | 149 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 159 | 159 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 162 | 162 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 336 | 336 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[KST] | ||||||||
Additional information
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| Size range | 536-734 amino acids |
| Related rules |
MF_01228 |
| Fusion | Nter: None Cter: None |
| Comments | It is possible that several species such as ARCFU, METAC, METMA, METKA, METTH, METJA and TREPA bind a second zinc ion, as their sequences contain a second zinc finger motif corresponding to that of metG subfamily 2 |