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HAMAP rule MF_00098

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General rule information [?]

Accession MF_00098
Dates 29-OCT-2002 (Created)
1-JUN-2023 (Last updated, Version 42)
Name Met_tRNA_synth_type1
Scope(s) Bacteria
Template(s) P00959 (SYM_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00098 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SYM
Protein name RecName: Full=Methionine--tRNA ligase;
AltName: Full=Methionyl-tRNA synthetase;
Gene name Name=metG;

Comments [?]

FUNCTIONIs required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.
CATALYTIC ACTIVITY Reaction=ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L- methionyl-tRNA(Met); Xref=Rhea:RHEA:13481, Rhea:RHEA-COMP:9667, Rhea:RHEA-COMP:9698, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57844, ChEBI:CHEBI:78442, ChEBI:CHEBI:78530, ChEBI:CHEBI:456215; EC=;
case <FTGroup:1>
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.;
end case
case <Feature:PS50886>
end case
case not <Feature:PS50886> and <OC:Bacteria>
end case
SIMILARITYBelongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0004825; Molecular function:methionine-tRNA ligase activity
GO:0006431; Biological process:methionyl-tRNA aminoacylation
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00133; tRNA-synt_1; 1;
NCBIfam TIGR00398; MetG; 1;
NCBIfam TIGR00399; MetG_C_term; 1;
PROSITE PS50886; TRBD; 0-1;

Features [?]

From: SYM_ECOLI (P00959)
Key From To Description Tag Condition FTGroup
MOTIF 15 25 /note="'HIGH' region" [PYA]-[YT]-x(5)-H-[LIVFA]-G-[HNST]
MOTIF 333 337 /note="'KMSKS' region" [KTQ]-[LIMF]-[SG]-[KTS]-[ST]
BINDING 146 146 /ligand="Zn(2+)"
C 1
BINDING 149 149 /ligand="Zn(2+)"
C 1
BINDING 159 159 /ligand="Zn(2+)"
C 1
BINDING 162 162 /ligand="Zn(2+)"
C 1
BINDING 336 336 /ligand="ATP"

Additional information [?]

Size range 536-734 amino acids
Related rules MF_01228
Fusion Nter: None Cter: None
Comments It is possible that several species such as ARCFU, METAC, METMA, METKA, METTH, METJA and TREPA bind a second zinc ion, as their sequences contain a second zinc finger motif corresponding to that of metG subfamily 2

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