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Annotation rule MF_00098
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General rule information [?]

Accession MF_00098
Dates 29-OCT-2002 (Created)
20-NOV-2019 (Last updated, Version 39)
Name Met_tRNA_synth_type1
Scope
Bacteria
Archaea
Template P00959 (SYM_ECOLI)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
SYM
Protein name
RecName: Full=Methionine--tRNA ligase;
EC 6.1.1.10;
AltName: Full=Methionyl-tRNA synthetase;
Short=MetRS;
Gene name
metG

Comments [?]

Function Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.
Catalytic activity RHEA:13481: ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
EC 6.1.1.10
case <FTGroup:1>
Cofactor Zn(2+)
Note: Binds 1 zinc ion per subunit.
end case
case <Feature:PS50886>
Subunit Homodimer.
end case
case not <Feature:PS50886> and <OC:Bacteria>
Subunit Monomer.
end case
Subcellular location Cytoplasm.
Similarity Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Keywords [?]

Cytoplasm
Aminoacyl-tRNA synthetase
Protein biosynthesis
Ligase
ATP-binding
Nucleotide-binding
case <Feature:PS50886>
RNA-binding
tRNA-binding
end case
case <FTGroup:1>
Metal-binding
Zinc
end case

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004825; Molecular function: methionine-tRNA ligase activity.
GO:0006431; Biological process: methionyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00133; tRNA-synt_1; 1;
PRINTS PR01041; TRNASYNTHMET; 1;
TIGRFAMs TIGR00398; MetG; 1;
TIGR00399; MetG_C_term; 1;
PROSITE PS00178; AA_TRNA_LIGASE_I; 1;
PS50886; TRBD; 0-1; trigger=PRU00209;

Features [?]

From: SYM_ECOLI (P00959)
Key     From     To       Description   Tag   Condition   FTGroup
MOTIF     15     25       'HIGH' region     [PYA]-[YT]-x(5)-H-[LIVFA]-G-[HNST]  
MOTIF     333     337       'KMSKS' region     [KTQ]-[LIMF]-[SG]-[KTS]-[ST]  
METAL     146     146       Zinc     C   1
METAL     149     149       Zinc     C   1
METAL     159     159       Zinc     C   1
METAL     162     162       Zinc     C   1
BINDING     336     336       ATP     [KST]  

Additional information [?]

Size range 536-734 amino acids
Related rules MF_01228 (SYM)
Fusion None
Comments It is possible that several species such as ARCFU, METAC, METMA, METKA, METTH, METJA and TREPA bind a second zinc ion, as their sequences contain a second zinc finger motif corresponding to that of metG subfamily 2


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