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Annotation rule MF_00099 |
Accession | MF_00099 |
Dates | 1-JUN-2001 (Created) 20-NOV-2019 (Last updated, Version 61) |
Name | CheB_chemtxs |
Scope | Bacteria
Archaea |
Templates | P07330 (CHEB_ECOLI); P04042 (CHEB_SALTY); O87125 (CHEB1_PSEAE); Q9I6V9 (CHEB2_PSEAE): [Recover all] |
Triggered by |
Identifier |
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Protein name |
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Gene name |
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Function | Involved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid. |
Catalytic activity | RHEA:23236: [protein]-L-glutamate 5-O-methyl ester + H2O = H(+) + L-glutamyl-[protein] + methanol
EC 3.1.1.61 |
RHEA:16441: H2O + L-glutaminyl-[protein] = L-glutamyl-[protein] + NH4(+)
EC 3.5.1.44 |
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Subcellular location | Cytoplasm. |
Domain | Contains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity. |
Ptm | Phosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity. |
Similarity | Belongs to the CheB family. |
Pfam | PF01339; CheB_methylest; 1; |
PF00072; Response_reg; 1; | |
PIRSF | PIRSF000876; RR_chemtxs_CheB; 1; |
PROSITE | PS50122; CHEB; 1; trigger=PRU00050; |
PS50110; RESPONSE_REGULATORY; 1; trigger=PRU00169; |
From: CHEB_ECOLI (P07330) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 164 | 164 | S | |||||||||
ACT_SITE | 190 | 190 | H | |||||||||
ACT_SITE | 286 | 286 | D | |||||||||
MOD_RES | 56 | 56 | 4-aspartylphosphate | phospho | D |
Size range | 334-424 amino acids |
Related rules | None |
Fusion | None |
Comments | Gammaproteobacteria gene names for multiple proteins are based on the characterized PSEAE genes, see: PubMed=12142407; Ferrandez A., Hawkins A.C., Summerfield D.T., Harwood C.S.; "Cluster II che genes from Pseudomonas aeruginosa are required for an optimal chemotactic response."; J. Bacteriol. 184:4374-4383(2002). Alphaproteobacteria gene names for multiple proteins are based on operon organization as discussed in: PubMed=11934495; Hauwaerts D., Alexandre G., Das S.K., Vanderleyden J., Zhulin I.B.; "A major chemotaxis gene cluster in Azospirillum brasilense and relationships between chemotaxis operons in alpha-proteobacteria."; FEMS Microbiol. Lett. 208:61-67(2002). There are cheB-like proteins without the N-terminal regulatory domain in MYXXA (frzB), BORBU (BB0415), LEPIC (cheB2) and LEPIN (cheB2). CHEB3_RHOSH is missing the conserved D for phosphorylation. Possible wrong start in RHOCE |