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| Annotation rule MF_00099 |
General rule information
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| Accession | MF_00099 |
| Dates | 1-JUN-2001 (Created) 20-NOV-2019 (Last updated, Version 61) |
| Name | CheB_chemtxs |
| Scope | Bacteria
Archaea |
| Templates | P07330 (CHEB_ECOLI); P04042 (CHEB_SALTY); O87125 (CHEB1_PSEAE); Q9I6V9 (CHEB2_PSEAE): [Recover all] |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | Involved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid. |
| Catalytic activity | RHEA:23236: [protein]-L-glutamate 5-O-methyl ester + H2O = H(+) + L-glutamyl-[protein] + methanol
EC 3.1.1.61 |
| RHEA:16441: H2O + L-glutaminyl-[protein] = L-glutamyl-[protein] + NH4(+)
EC 3.5.1.44 |
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| Subcellular location | Cytoplasm. |
| Domain | Contains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity. |
case <FTTag:phospho>
| Ptm | Phosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity. |
end case
| Similarity | Belongs to the CheB family. |
Keywords
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case <FTTag:phospho>
end case
Gene Ontology
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GO:0008984; Molecular function: protein-glutamate methylesterase activity.
GO:0050568; Molecular function: protein-glutamine glutaminase activity.
GO:0006935; Biological process: chemotaxis.
GO:0007165; Biological process: signal transduction.
GO:0000160; Biological process: phosphorelay signal transduction system.
GO:0018277; Biological process: protein deamination.
GO:0006482; Biological process: protein demethylation.
GO:0005737; Cellular component: cytoplasm.
GO:0050568; Molecular function: protein-glutamine glutaminase activity.
GO:0006935; Biological process: chemotaxis.
GO:0007165; Biological process: signal transduction.
GO:0000160; Biological process: phosphorelay signal transduction system.
GO:0018277; Biological process: protein deamination.
GO:0006482; Biological process: protein demethylation.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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| Pfam | PF01339; CheB_methylest; 1; |
| PF00072; Response_reg; 1; | |
| PIRSF | PIRSF000876; RR_chemtxs_CheB; 1; |
| PROSITE | PS50122; CHEB; 1; trigger=PRU00050; |
| PS50110; RESPONSE_REGULATORY; 1; trigger=PRU00169; |
Features
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| From: CHEB_ECOLI (P07330) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 164 | 164 | S | |||||||||
| ACT_SITE | 190 | 190 | H | |||||||||
| ACT_SITE | 286 | 286 | D | |||||||||
| MOD_RES | 56 | 56 | 4-aspartylphosphate | phospho | D | |||||||
Additional information
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| Size range | 334-424 amino acids |
| Related rules | None |
| Fusion | None |
| Comments | Gammaproteobacteria gene names for multiple proteins are based on the characterized PSEAE genes, see: PubMed=12142407; Ferrandez A., Hawkins A.C., Summerfield D.T., Harwood C.S.; "Cluster II che genes from Pseudomonas aeruginosa are required for an optimal chemotactic response."; J. Bacteriol. 184:4374-4383(2002). Alphaproteobacteria gene names for multiple proteins are based on operon organization as discussed in: PubMed=11934495; Hauwaerts D., Alexandre G., Das S.K., Vanderleyden J., Zhulin I.B.; "A major chemotaxis gene cluster in Azospirillum brasilense and relationships between chemotaxis operons in alpha-proteobacteria."; FEMS Microbiol. Lett. 208:61-67(2002). There are cheB-like proteins without the N-terminal regulatory domain in MYXXA (frzB), BORBU (BB0415), LEPIC (cheB2) and LEPIN (cheB2). CHEB3_RHOSH is missing the conserved D for phosphorylation. Possible wrong start in RHOCE |