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HAMAP rule MF_00099

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General rule information [?]

Accession MF_00099
Dates 1-JUN-2001 (Created)
28-NOV-2023 (Last updated, Version 65)
Name CheB_chemtxs
Scope(s) Bacteria
Template(s) P07330 (CHEB_ECOLI); P04042 (CHEB_SALTY); O87125 (CHEB1_PSEAE); Q9I6V9 (CHEB2_PSEAE); [ Recover all ]
Triggered by HAMAP; MF_00099 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier CHEB
Protein name RecName: Full=Protein-glutamate methylesterase/protein-glutamine glutaminase;
Gene name Name=cheB;

Comments [?]

FUNCTIONInvolved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid.
CATALYTIC ACTIVITY Reaction=[protein]-L-glutamate 5-O-methyl ester + H2O = H(+) + L- glutamyl-[protein] + methanol; Xref=Rhea:RHEA:23236, Rhea:RHEA- COMP:10208, Rhea:RHEA-COMP:10311, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:29973, ChEBI:CHEBI:82795; EC=;
CATALYTIC ACTIVITY Reaction=H2O + L-glutaminyl-[protein] = L-glutamyl-[protein] + NH4(+); Xref=Rhea:RHEA:16441, Rhea:RHEA-COMP:10207, Rhea:RHEA-COMP:10208, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:29973, ChEBI:CHEBI:30011; EC=;
DOMAINContains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity.
case <FTTag:phospho>
PTMPhosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity.
end case
SIMILARITYBelongs to the CheB family.

Keywords [?]

case <FTTag:phospho>
end case

Gene Ontology [?]

GO:0008984; Molecular function:protein-glutamate methylesterase activity
GO:0050568; Molecular function:protein-glutamine glutaminase activity
GO:0006935; Biological process:chemotaxis
GO:0007165; Biological process:signal transduction
GO:0000160; Biological process:phosphorelay signal transduction system
GO:0018277; Biological process:protein deamination
GO:0006482; Biological process:protein demethylation
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF01339; CheB_methylest; 1;
Pfam PF00072; Response_reg; 1;
PIRSF PIRSF000876; RR_chemtxs_CheB; 1;

Features [?]

From: CHEB_ECOLI (P07330)
Key From To Description Tag Condition FTGroup
ACT_SITE 164 164 S
ACT_SITE 190 190 H
ACT_SITE 286 286 D
MOD_RES 56 56 /note="4-aspartylphosphate" phospho D

Additional information [?]

Size range 334-424 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Gammaproteobacteria gene names for multiple proteins are based on the characterized PSEAE genes, see: PubMed=12142407; Ferrandez A., Hawkins A.C., Summerfield D.T., Harwood C.S.; "Cluster II che genes from Pseudomonas aeruginosa are required for an optimal chemotactic response."; J. Bacteriol. 184:4374-4383(2002). Alphaproteobacteria gene names for multiple proteins are based on operon organization as discussed in: PubMed=11934495; Hauwaerts D., Alexandre G., Das S.K., Vanderleyden J., Zhulin I.B.; "A major chemotaxis gene cluster in Azospirillum brasilense and relationships between chemotaxis operons in alpha-proteobacteria."; FEMS Microbiol. Lett. 208:61-67(2002). There are cheB-like proteins without the N-terminal regulatory domain in MYXXA (frzB), BORBU (BB0415), LEPIC (cheB2) and LEPIN (cheB2). CHEB3_RHOSH is missing the conserved D for phosphorylation. Possible wrong start in RHOCE

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