HAMAP rule MF_00099

General rule information [?]

Accession	MF_00099
Dates	1-JUN-2001 (Created) 23-JAN-2023 (Last updated, Version 63)
Name	CheB_chemtxs
Scope(s)	Bacteria Archaea
Template(s)	P07330 (CHEB_ECOLI); P04042 (CHEB_SALTY); O87125 (CHEB1_PSEAE); Q9I6V9 (CHEB2_PSEAE); [ Recover all ]
Triggered by	HAMAP; MF_00099 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	CHEB
Protein name	RecName: Full=Protein-glutamate methylesterase/protein-glutamine glutaminase; EC=3.1.1.61; EC=3.5.1.44;
Gene name	Name=cheB;

Comments [?]

FUNCTION	Involved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid.
CATALYTIC ACTIVITY	Reaction=[protein]-L-glutamate 5-O-methyl ester + H2O = H(+) + L- glutamyl-[protein] + methanol; Xref=Rhea:RHEA:23236, Rhea:RHEA- COMP:10208, Rhea:RHEA-COMP:10311, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:29973, ChEBI:CHEBI:82795; EC=3.1.1.61;
CATALYTIC ACTIVITY	Reaction=H2O + L-glutaminyl-[protein] = L-glutamyl-[protein] + NH4(+); Xref=Rhea:RHEA:16441, Rhea:RHEA-COMP:10207, Rhea:RHEA-COMP:10208, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:29973, ChEBI:CHEBI:30011; EC=3.5.1.44;
SUBCELLULAR LOCATION	Cytoplasm.
DOMAIN	Contains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity.
case <FTTag:phospho>
PTM	Phosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity.
end case
SIMILARITY	Belongs to the CheB family.

Keywords [?]

Gene Ontology [?]

GO:0008984; Molecular function:protein-glutamate methylesterase activity

GO:0050568; Molecular function:protein-glutamine glutaminase activity

GO:0006935; Biological process:chemotaxis

GO:0007165; Biological process:signal transduction

GO:0000160; Biological process:phosphorelay signal transduction system

GO:0018277; Biological process:protein deamination

GO:0006482; Biological process:protein demethylation

GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Features [?]

From: CHEB_ECOLI (P07330)

Key

From

Description

Tag

Condition

FTGroup

ACT_SITE

164

ACT_SITE

190

ACT_SITE

286

MOD_RES

/note="4-aspartylphosphate"

phospho

Additional information [?]

Size range	334-424 amino acids
Related rules	None
Fusion	Nter: None Cter: None
Comments	Gammaproteobacteria gene names for multiple proteins are based on the characterized PSEAE genes, see: PubMed=12142407; Ferrandez A., Hawkins A.C., Summerfield D.T., Harwood C.S.; "Cluster II che genes from Pseudomonas aeruginosa are required for an optimal chemotactic response."; J. Bacteriol. 184:4374-4383(2002). Alphaproteobacteria gene names for multiple proteins are based on operon organization as discussed in: PubMed=11934495; Hauwaerts D., Alexandre G., Das S.K., Vanderleyden J., Zhulin I.B.; "A major chemotaxis gene cluster in Azospirillum brasilense and relationships between chemotaxis operons in alpha-proteobacteria."; FEMS Microbiol. Lett. 208:61-67(2002). There are cheB-like proteins without the N-terminal regulatory domain in MYXXA (frzB), BORBU (BB0415), LEPIC (cheB2) and LEPIN (cheB2). CHEB3_RHOSH is missing the conserved D for phosphorylation. Possible wrong start in RHOCE