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Annotation rule MF_00099
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General rule information [?]

Accession MF_00099
Dates 1-JUN-2001 (Created)
20-NOV-2019 (Last updated, Version 61)
Name CheB_chemtxs
Templates P07330 (CHEB_ECOLI); P04042 (CHEB_SALTY); O87125 (CHEB1_PSEAE); Q9I6V9 (CHEB2_PSEAE): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Protein-glutamate methylesterase/protein-glutamine glutaminase;
Gene name

Comments [?]

Function Involved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid.
Catalytic activity RHEA:23236: [protein]-L-glutamate 5-O-methyl ester + H2O = H(+) + L-glutamyl-[protein] + methanol
RHEA:16441: H2O + L-glutaminyl-[protein] = L-glutamyl-[protein] + NH4(+)
Subcellular location Cytoplasm.
Domain Contains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity.
case <FTTag:phospho>
Ptm Phosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity.
end case
Similarity Belongs to the CheB family.

Keywords [?]

case <FTTag:phospho>
end case

Gene Ontology [?]

GO:0008984; Molecular function: protein-glutamate methylesterase activity.
GO:0050568; Molecular function: protein-glutamine glutaminase activity.
GO:0006935; Biological process: chemotaxis.
GO:0007165; Biological process: signal transduction.
GO:0000160; Biological process: phosphorelay signal transduction system.
GO:0018277; Biological process: protein deamination.
GO:0006482; Biological process: protein demethylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF01339; CheB_methylest; 1;
PF00072; Response_reg; 1;
PIRSF PIRSF000876; RR_chemtxs_CheB; 1;
PROSITE PS50122; CHEB; 1; trigger=PRU00050;
PS50110; RESPONSE_REGULATORY; 1; trigger=PRU00169;

Features [?]

From: CHEB_ECOLI (P07330)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     164     164             S  
ACT_SITE     190     190             H  
ACT_SITE     286     286             D  
MOD_RES     56     56       4-aspartylphosphate   phospho   D  

Additional information [?]

Size range 334-424 amino acids
Related rules None
Fusion None
Comments Gammaproteobacteria gene names for multiple proteins are based on the characterized PSEAE genes, see: PubMed=12142407; Ferrandez A., Hawkins A.C., Summerfield D.T., Harwood C.S.; "Cluster II che genes from Pseudomonas aeruginosa are required for an optimal chemotactic response."; J. Bacteriol. 184:4374-4383(2002). Alphaproteobacteria gene names for multiple proteins are based on operon organization as discussed in: PubMed=11934495; Hauwaerts D., Alexandre G., Das S.K., Vanderleyden J., Zhulin I.B.; "A major chemotaxis gene cluster in Azospirillum brasilense and relationships between chemotaxis operons in alpha-proteobacteria."; FEMS Microbiol. Lett. 208:61-67(2002). There are cheB-like proteins without the N-terminal regulatory domain in MYXXA (frzB), BORBU (BB0415), LEPIC (cheB2) and LEPIN (cheB2). CHEB3_RHOSH is missing the conserved D for phosphorylation. Possible wrong start in RHOCE