HAMAP rule MF_00102

General rule information [?]

Accession	MF_00102
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 41)

Name

DapB

Scope

Bacteria

Archaea

Templates

P04036 (DAPB_ECOLI); P9WP23 (DAPB_MYCTU); Q9X1K8 (DAPB_THEMA): [Recover all]

Triggered by

HAMAP; MF_00102 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

DAPB

Protein name

RecName:		Full=4-hydroxy-tetrahydrodipicolinate reductase;
		Short=HTPA reductase;
		EC 1.17.1.8;

Gene name

dapB

Comments [?]

Function	Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.
Catalytic activity	RHEA:35323: (S)-2,3,4,5-tetrahydrodipicolinate + H2O + NAD(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADH EC 1.17.1.8
	RHEA:35331: (S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH EC 1.17.1.8
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4.
Subunit	Homotetramer.
Subcellular location	Cytoplasm.
Similarity	Belongs to the DapB family.
Caution	Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.

Keywords [?]

Cytoplasm
Amino-acid biosynthesis
Diaminopimelate biosynthesis
Lysine biosynthesis
Oxidoreductase
NAD
NADP

Gene Ontology [?]

GO:0016726; Molecular function: oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor.
GO:0050661; Molecular function: NADP binding.
GO:0051287; Molecular function: NAD binding.
GO:0019877; Biological process: diaminopimelate biosynthetic process.
GO:0009089; Biological process: lysine biosynthetic process via diaminopimelate.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam	PF05173; DapB_C; 1;
	PF01113; DapB_N; 1;
NCBIfam	TIGR00036; DapB; 1;
PROSITE	PS01298; DAPB; 1;

Features [?]

From: DAPB_ECOLI (P04036)

Key From To Description Tag Condition FTGroup

BINDING 12 17 /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540 G-x-x-G-x-x

BINDING 102 104 /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540 [GAC]-x-[TS]

BINDING (Optional) 126 129 /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540 [ASTGCV]-x-x-[FYMTVL]

BINDING 169 170 /ligand="(S)-2,3,4,5-tetrahydrodipicolinate" /ligand_id="ChEBI:CHEBI:16845 [GA]-[TS]

ACT_SITE 159 159 Proton donor/acceptor H

ACT_SITE 163 163 Proton donor K

BINDING (Optional) 38 38 /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540 [ED]

BINDING (Optional) 39 39 /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349 [RK]

BINDING (Optional) 160 160 /ligand="(S)-2,3,4,5-tetrahydrodipicolinate" /ligand_id="ChEBI:CHEBI:16845 [HR]

Additional information [?]

Size range	216-283 amino acids
Related rules	None
Fusion	None
Comments	MYCBO strain BCG seems to originate from a different bacterial species; its sequence is too divergent to that of MYCBO strain AF2122/97 and MYCTU

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