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HAMAP rule MF_00102

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General rule information [?]

Accession MF_00102
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 41)
Name DapB
Scope(s) Bacteria
Template(s) P04036 (DAPB_ECOLI); P9WP23 (DAPB_MYCTU); Q9X1K8 (DAPB_THEMA); [ Recover all ]
Triggered by HAMAP; MF_00102 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier DAPB
Protein name RecName: Full=4-hydroxy-tetrahydrodipicolinate reductase;
                 Short=HTPA reductase;
Gene name Name=dapB;

Comments [?]

FUNCTIONCatalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.
CATALYTIC ACTIVITY Reaction=(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NAD(+) = (2S,4S)-4- hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADH; Xref=Rhea:RHEA:35323, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16845, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:67139; EC=;
CATALYTIC ACTIVITY Reaction=(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)- 4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH; Xref=Rhea:RHEA:35331, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16845, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:67139; EC=;
PATHWAYAmino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4.
SIMILARITYBelongs to the DapB family.
CAUTIONWas originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.

Keywords [?]

Gene Ontology [?]

GO:0016726; Molecular function:oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor
GO:0050661; Molecular function:NADP binding
GO:0051287; Molecular function:NAD binding
GO:0019877; Biological process:diaminopimelate biosynthetic process
GO:0009089; Biological process:lysine biosynthetic process via diaminopimelate
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF05173; DapB_C; 1;
Pfam PF01113; DapB_N; 1;
NCBIfam TIGR00036; DapB; 1;

Features [?]

From: DAPB_ECOLI (P04036)
Key From To Description Tag Condition FTGroup
BINDING 12 17 /ligand="NAD(+)"
BINDING 102 104 /ligand="NAD(+)"
BINDING 126 129 /ligand="NAD(+)"
BINDING 169 170 /ligand="(S)-2,3,4,5-tetrahydrodipicolinate"
ACT_SITE 159 159 /note="Proton donor/acceptor" H
ACT_SITE 163 163 /note="Proton donor" K
BINDING 38 38 /ligand="NAD(+)"
BINDING 39 39 /ligand="NADP(+)"
BINDING 160 160 /ligand="(S)-2,3,4,5-tetrahydrodipicolinate"

Additional information [?]

Size range 216-283 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments MYCBO strain BCG seems to originate from a different bacterial species; its sequence is too divergent to that of MYCBO strain AF2122/97 and MYCTU

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