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Annotation rule MF_00102 |
Accession | MF_00102 |
Dates | 1-JUN-2001 (Created) 18-NOV-2019 (Last updated, Version 36) |
Name | DapB |
Scope | Bacteria
Archaea |
Templates | P04036 (DAPB_ECOLI); P9WP23 (DAPB_MYCTU); Q9X1K8 (DAPB_THEMA): [Recover all] |
Triggered by |
Identifier |
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Protein name |
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Gene name |
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Function | Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate. |
Catalytic activity | RHEA:35323: (S)-2,3,4,5-tetrahydrodipicolinate + H2O + NAD(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADH
EC 1.17.1.8 |
RHEA:35331: (S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH
EC 1.17.1.8 |
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Pathway | Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4. |
Subunit | Homotetramer. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the DapB family. |
Caution | Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction. |
From: DAPB_ECOLI (P04036) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
NP_BIND | 12 | 17 | NAD(P) | G-x-x-G-x-x | ||||||||
NP_BIND | 102 | 104 | NAD(P) | [GAC]-x-[TS] | ||||||||
NP_BIND (Optional) | 126 | 129 | NAD(P) | [ASTGCV]-x-x-[FYMTVL] | ||||||||
REGION | 169 | 170 | Substrate binding | [GA]-[TS] | ||||||||
ACT_SITE | 159 | 159 | Proton donor/acceptor | H | ||||||||
ACT_SITE | 163 | 163 | Proton donor | K | ||||||||
BINDING (Optional) | 38 | 38 | NAD | [ED] | ||||||||
BINDING (Optional) | 39 | 39 | NADP | [RK] | ||||||||
BINDING (Optional) | 160 | 160 | Substrate | [HR] |
Size range | 216-283 amino acids |
Related rules | None |
Fusion | None |
Comments | MYCBO strain BCG seems to originate from a different bacterial species; its sequence is too divergent to that of MYCBO strain AF2122/97 and MYCTU |