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Annotation rule MF_00102
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General rule information [?]

Accession MF_00102
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 36)
Name DapB
Scope
Bacteria
Archaea
Templates P04036 (DAPB_ECOLI); P9WP23 (DAPB_MYCTU); Q9X1K8 (DAPB_THEMA): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
DAPB
Protein name
RecName: Full=4-hydroxy-tetrahydrodipicolinate reductase;
Short=HTPA reductase;
EC 1.17.1.8;
Gene name
dapB

Comments [?]

Function Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.
Catalytic activity RHEA:35323: (S)-2,3,4,5-tetrahydrodipicolinate + H2O + NAD(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADH
EC 1.17.1.8
RHEA:35331: (S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH
EC 1.17.1.8
Pathway Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4.
Subunit Homotetramer.
Subcellular location Cytoplasm.
Similarity Belongs to the DapB family.
Caution Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.

Keywords [?]

Cytoplasm
Amino-acid biosynthesis
Diaminopimelate biosynthesis
Lysine biosynthesis
Oxidoreductase
NAD
NADP

Gene Ontology [?]

GO:0016726; Molecular function: oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor.
GO:0050661; Molecular function: NADP binding.
GO:0051287; Molecular function: NAD binding.
GO:0019877; Biological process: diaminopimelate biosynthetic process.
GO:0009089; Biological process: lysine biosynthetic process via diaminopimelate.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF05173; DapB_C; 1;
PF01113; DapB_N; 1;
TIGRFAMs TIGR00036; DapB; 1;
PROSITE PS01298; DAPB; 1;

Features [?]

From: DAPB_ECOLI (P04036)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     12     17       NAD(P)     G-x-x-G-x-x  
NP_BIND     102     104       NAD(P)     [GAC]-x-[TS]  
NP_BIND (Optional)     126     129       NAD(P)     [ASTGCV]-x-x-[FYMTVL]  
REGION     169     170       Substrate binding     [GA]-[TS]  
ACT_SITE     159     159       Proton donor/acceptor     H  
ACT_SITE     163     163       Proton donor     K  
BINDING (Optional)     38     38       NAD     [ED]  
BINDING (Optional)     39     39       NADP     [RK]  
BINDING (Optional)     160     160       Substrate     [HR]  

Additional information [?]

Size range 216-283 amino acids
Related rules None
Fusion None
Comments MYCBO strain BCG seems to originate from a different bacterial species; its sequence is too divergent to that of MYCBO strain AF2122/97 and MYCTU


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