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Annotation rule MF_00103
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General rule information [?]

Accession MF_00103
Dates 1-JUN-2001 (Created)
8-JUN-2021 (Last updated, Version 43)
Name Fapy_DNA_glycosyl
Templates P05523 (FPG_ECOLI); P42371 (FPG_LACLC); O50606 (FPG_THET8): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Formamidopyrimidine-DNA glycosylase;
Short=Fapy-DNA glycosylase;
AltName: Full=DNA-(apurinic or apyrimidinic site) lyase MutM;
Short=AP lyase MutM;
Gene name
mutM, fpg

Comments [?]

Function Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.
Catalytic activity Reaction=Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.; EC=;
RHEA:66592: (DNA)-(deoxyribonucleoside 5'-phosphate)-(deoxyribose 5'-phosphate)-(deoxyribonucleoside 5'-phosphate) = (DNA)-3'-(deoxyribonucleoside 5'-phosphate)-(2,3-dehydro-2,3-deoxyribose 5'-phosphate) + H(+) + phospho-5'-(DNA)
Cofactor Zn(2+)
Note: Binds 1 zinc ion per subunit.
Subunit Monomer.
Similarity Belongs to the FPG family.

Keywords [?]

case <Feature:PS51066>
end case

Gene Ontology [?]

GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) endonuclease activity.
GO:0008534; Molecular function: oxidized purine nucleobase lesion DNA N-glycosylase activity.
case <Feature:PS51066>
GO:0008270; Molecular function: zinc ion binding.
end case
GO:0006281; Biological process: DNA repair.

Cross-references [?]

Pfam PF01149; Fapy_DNA_glyco; 1;
PF06827; zf-FPG_IleRS; 1;
TIGRFAMs TIGR00577; Fpg; 1;
PS01242; ZF_FPG_1; 1;
PS51066; ZF_FPG_2; 1; trigger=PRU00391;

Features [?]

From: FPG_ECOLI (P05523)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     2     2       Schiff-base intermediate with DNA     P  
ACT_SITE     3     3       Proton donor     E  
ACT_SITE     57     57       Proton donor; for beta-elimination activity     K  
ACT_SITE     259     259       Proton donor; for delta-elimination activity     R  
BINDING     90     90       DNA     H  
BINDING     109     109       DNA     R  
BINDING     150     150       DNA     [KR]  

Additional information [?]

Size range 262-309 amino acids
Related rules None
Fusion None
Comments Weird insert in DESVH (not taken into account in size range)