HAMAP rule MF_00103
General rule information
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| Accession | MF_00103 |
| Dates | 1-JUN-2001 (Created)
30-JAN-2024 (Last updated, Version 49) |
| Name | Fapy_DNA_glycosyl |
| Scope(s) |
Bacteria |
| Template(s) | P05523 (FPG_ECOLI); P42371 (FPG_LACLC); O50606 (FPG_THET8); [ Recover all ] |
| Triggered by |
HAMAP; MF_00103 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | FPG |
| Protein name | RecName: Full=Formamidopyrimidine-DNA glycosylase; Short=Fapy-DNA glycosylase; EC=3.2.2.23; AltName: Full=DNA-(apurinic or apyrimidinic site) lyase MutM; Short=AP lyase MutM; EC=4.2.99.18; |
| Gene name | Name=mutM; Synonyms=fpg; |
Comments
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| FUNCTION | Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. |
| CATALYTIC ACTIVITY | Reaction=Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N- methyl)formamidopyrimidine.; EC=3.2.2.23; |
| CATALYTIC ACTIVITY | Reaction=2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'- deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'- deoxyribonucleoside-DNA + H(+); Xref=Rhea:RHEA:66592, Rhea:RHEA- COMP:13180, Rhea:RHEA-COMP:16897, Rhea:RHEA-COMP:17067, ChEBI:CHEBI:15378, ChEBI:CHEBI:136412, ChEBI:CHEBI:157695, ChEBI:CHEBI:167181; EC=4.2.99.18; |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.; |
| SUBUNIT | Monomer. |
| SIMILARITY | Belongs to the FPG family. |
Keywords
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| DNA damage | |
| DNA repair | |
| DNA-binding | |
| Hydrolase | |
| Glycosidase | |
| Lyase | |
| Multifunctional enzyme | |
| case <Feature:PS51066> | |
| Metal-binding | |
| Zinc | |
| Zinc-finger | |
| end case | |
Gene Ontology
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| GO:0003906; Molecular function:DNA-(apurinic or apyrimidinic site) endonuclease activity | |
| GO:0008534; Molecular function:oxidized purine nucleobase lesion DNA N-glycosylase activity | |
| case <Feature:PS51066> | |
| GO:0008270; Molecular function:zinc ion binding | |
| end case | |
| GO:0006281; Biological process:DNA repair | |
Cross-references
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| Pfam | PF01149; Fapy_DNA_glyco; 1; |
| Pfam | PF06827; zf-FPG_IleRS; 1; |
| NCBIfam | TIGR00577; Fpg; 1; |
| PROSITE | PS51068; FPG_CAT; 1; |
| PROSITE | PS01242; ZF_FPG_1; 1; |
| PROSITE | PS51066; ZF_FPG_2; 1; |
Features
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| From: FPG_ECOLI (P05523) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 2 | 2 | /note="Schiff-base intermediate with DNA" | P | ||||||||
| ACT_SITE | 3 | 3 | /note="Proton donor" | E | ||||||||
| ACT_SITE | 57 | 57 | /note="Proton donor; for beta-elimination activity" | K | ||||||||
| ACT_SITE | 259 | 259 | /note="Proton donor; for delta-elimination activity" | R | ||||||||
| BINDING | 90 | 90 | /ligand="DNA" /ligand_id="ChEBI:CHEBI:16991" |
H | ||||||||
| BINDING | 109 | 109 | /ligand="DNA" /ligand_id="ChEBI:CHEBI:16991" |
R | ||||||||
| BINDING | 150 | 150 | /ligand="DNA" /ligand_id="ChEBI:CHEBI:16991" |
[KR] | ||||||||
Additional information
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| Size range | 262-309 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Weird insert in DESVH (not taken into account in size range) |