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Annotation rule MF_00109
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General rule information [?]

Accession MF_00109
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 42)
Name Shikimate_kinase
Scope
Bacteria
Templates P0A6D7 (AROK_ECOLI); P9WPY3 (AROK_MYCTU): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
AROK
case not <OC:Enterobacterales>
Protein name
RecName: Full=Shikimate kinase;
Short=SK;
EC 2.7.1.71;
else case <OC:Enterobacterales>
Protein name
RecName: Full=Shikimate kinase 1;
Short=SK 1;
EC 2.7.1.71;
end case
Gene name
aroK

Comments [?]

Function Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.
Catalytic activity RHEA:13121: ATP + shikimate = 3-phosphoshikimate + ADP + H(+)
EC 2.7.1.71
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
Pathway Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.
Subunit Monomer.
Subcellular location Cytoplasm.
Similarity Belongs to the shikimate kinase family.

Keywords [?]


Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0005524; Molecular function: ATP binding.
GO:0004765; Molecular function: shikimate kinase activity.
GO:0009073; Biological process: aromatic amino acid family biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF01202; SKI; 1;
PRINTS PR01100; SHIKIMTKNASE; 1;
PROSITE PS01128; SHIKIMATE_KINASE; 1;

Features [?]

From: AROK_MYCTU (P9WPY3)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     12     17       ATP        
METAL     16     16       Magnesium     [ST]  
BINDING     34     34       Substrate     D  
BINDING     58     58       Substrate     R  
BINDING     80     80       Substrate; via amide nitrogen     G  
BINDING     117     117       ATP     [RK]  
BINDING     136     136       Substrate     R  
BINDING (Optional)     153     153       ATP     [RQ]  

Additional information [?]

Size range 161-214 amino acids
Related rules MF_01269 (AROL supersedes the current rule); MF_03143 (ARO1 supersedes the current rule)
Fusion Nter: None; Cter: MF_00110 (aroB)
Comments Archaeal aroK belong to a separate family (MF_00370). Enterobacterales (except WIGBR, BLOBP, BLOFL, BUCAI, BUCAP and BUCBP) possess a second shikimate kinase (aroL, MF_01269). Mg(2+) is six-coordinated in shikimate kinase 1 (aroK, MF_00109) with direct interaction with two protein side-chains, whereas it is four-coordinated in shikimate kinase 2 (aroL, MF_01269) with direct interaction with only one protein side-chain. Strange longer C-terminus in FRACC; sequence not included in size range.