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HAMAP rule MF_00109

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General rule information [?]

Accession MF_00109
Dates 1-JUN-2001 (Created)
12-MAR-2024 (Last updated, Version 45)
Name Shikimate_kinase
Scope(s) Bacteria
Template(s) P0A6D7 (AROK_ECOLI); P9WPY3 (AROK_MYCTU); [ Recover all ]
Triggered by HAMAP; MF_00109 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier AROK
case not <OC:Enterobacterales>
Protein name RecName: Full=Shikimate kinase;
else case <OC:Enterobacterales>
Protein name RecName: Full=Shikimate kinase 1;
                 Short=SK 1;
end case
Gene name Name=aroK;

Comments [?]

FUNCTIONCatalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.
CATALYTIC ACTIVITY Reaction=ATP + shikimate = 3-phosphoshikimate + ADP + H(+); Xref=Rhea:RHEA:13121, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:36208, ChEBI:CHEBI:145989, ChEBI:CHEBI:456216; EC=;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
PATHWAYMetabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.
SIMILARITYBelongs to the shikimate kinase family.

Keywords [?]

Gene Ontology [?]

GO:0000287; Molecular function:magnesium ion binding
GO:0005524; Molecular function:ATP binding
GO:0004765; Molecular function:shikimate kinase activity
GO:0009423; Biological process:chorismate biosynthetic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF01202; SKI; 1;

Features [?]

Key From To Description Tag Condition FTGroup
BINDING 12 17 /ligand="ATP"
BINDING 16 16 /ligand="Mg(2+)"
BINDING 34 34 /ligand="substrate" D
BINDING 58 58 /ligand="substrate" R
BINDING 80 80 /ligand="substrate" G
BINDING 117 117 /ligand="ATP"
BINDING 136 136 /ligand="substrate" R
BINDING 153 153 /ligand="ATP"

Additional information [?]

Size range 161-214 amino acids
Related rules MF_01269
Fusion Nter: None Cter: MF_00110 (aroB)
Comments Archaeal aroK belong to a separate family (MF_00370). Enterobacterales (except WIGBR, BLOBP, BLOFL, BUCAI, BUCAP and BUCBP) possess a second shikimate kinase (aroL, MF_01269). Mg(2+) is six-coordinated in shikimate kinase 1 (aroK, MF_00109) with direct interaction with two protein side-chains, whereas it is four-coordinated in shikimate kinase 2 (aroL, MF_01269) with direct interaction with only one protein side-chain. Strange longer C-terminus in FRACC; sequence not included in size range.

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