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HAMAP rule MF_00118

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_00118
Accession MF_00118
Dates 1-JUN-2001 (Created)
3-DEC-2024 (Last updated, Version 47)
Name EF_Tu
Scope(s) Bacteria
Archaea
Plastid
Template(s) P35021 (EF1A_SACS2); P0CE47 (EFTU1_ECOLI); P0CE48 (EFTU2_ECOLI); Q01698 (EFTU_THEAQ); P60338 (EFTU1_THETH); Q5SHN6 (EFTU1_THET8); P60339 (EFTU2_THET8); [ Recover all ]
Triggered by
case c? <OC:Bacteria> or <OG:Chloroplast>
HAMAP; MF_00118_B (Get profile general information and statistics)
end case
case c? <OC:Archaea>
HAMAP; MF_00118_A (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Bacteria>
Identifier EFTU
Protein name RecName: Full=Elongation factor Tu;
                 Short=EF-Tu;
                 EC=3.6.5.3;
Gene name Name=tuf;
else case <OC:Archaea>
Identifier EF1A
Protein name RecName: Full=Elongation factor 1-alpha;
                 Short=EF-1-alpha;
                 EC=3.6.5.3;
AltName: Full=Elongation factor Tu;
                 Short=EF-Tu;
Gene name Name=tuf;
else case <OG:Chloroplast>
Identifier EFTU
Protein name RecName: Full=Elongation factor Tu, chloroplastic;
                 Short=EF-Tu;
                 EC=3.6.5.3;
Gene name Name=tufA;
end case

Comments [?]

FUNCTIONGTP hydrolase that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
CATALYTIC ACTIVITY Reaction=GTP + H2O = GDP + phosphate + H(+); Xref=Rhea:RHEA:19669, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.3; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19670;
case <OC:Bacteria>
SUBUNITMonomer.
SUBCELLULAR LOCATIONCytoplasm.
end case
case <OC:Archaea>
SUBCELLULAR LOCATIONCytoplasm.
end case
case <OG:Chloroplast>
SUBCELLULAR LOCATIONPlastid, chloroplast.
end case
SIMILARITYBelongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.

Keywords [?]


Gene Ontology [?]

GO:0005525; Molecular function:GTP binding
GO:0003746; Molecular function:translation elongation factor activity
GO:0003924; Molecular function:GTPase activity
GO:0000287; Molecular function:magnesium ion binding
case <OCellular component:Bacteria> or <OC:Archaea>
GO:0005737; Cellular component:cytoplasm
end case
case <OG:Chloroplast>
GO:0009507; Cellular component:chloroplast
end case

Cross-references [?]

Pfam PF00009; GTP_EFTU; 1;
PRINTS PR00315; ELONGATNFCT; 1;
NCBIfam TIGR00483; EF-1_alpha; 1;
NCBIfam TIGR00485; EF-Tu; 1;
PROSITE PS00301; G_TR_1; 1;
PROSITE PS51722; G_TR_2; 1;

Features [?]

From: EFTU1_ECOLI (P0CE47)
Key From To Description Tag Condition FTGroup
case <OC:Bacteria> or <OG:Chloroplast>
BINDING 26 26 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
[ST]
BINDING 19 26 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"
G-[HQ]-[IV]-[DY]-H-G-K-[ST]
BINDING 81 85 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"
D-x-P-G-H
BINDING 136 139 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"
N-K-x-D
end case
From: EF1A_METJA (Q57770)
Key From To Description Tag Condition FTGroup
case <OC:Archaea>
BINDING 21 21 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
[ST]
BINDING 14 21 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"
G-H-[IV]-D-[NHA]-G-K-S
BINDING 91 95 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"
D-x-P-G-H
BINDING 149 152 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"
N-K-M-D
end case

Additional information [?]

Size range 350-550 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Duplicated in some but not necessarily all Acidobacteriota, Pseudomonadota, Alkaliohilus, Clostrium, Chloroflexota, Deinococcus, Magnetococcus, Sphingobacter, Streptomyces, Syntrophobacter. Short versions in EHRRW, VIBVU, and a divergent copy in CHAGL are atypical.



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