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Annotation rule MF_00121
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General rule information [?]

Accession MF_00121
Dates 1-JUN-2001 (Created)
27-OCT-2018 (Last updated, Version 26)
Name GatB
Scope
Bacteria
Archaea
Templates O30509 (GATB_BACSU); Q9LCX2 (GATB_THET8); O27341 (GATB_METTH): [Recover all]
case <OC:Bacteria> or <OC:Archaea>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
GATB
Protein name
RecName: Full=Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B;
Short=Asp/Glu-ADT subunit B;
EC=6.3.5.-;
Gene name
gatB

Comments [?]

Function Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).
Catalytic activity Reaction=ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate; Xref=Rhea:RHEA:17521, Rhea:RHEA-COMP:9681, Rhea:RHEA-COMP:9684, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:78520, ChEBI:CHEBI:78521, ChEBI:CHEBI:456216;.
Reaction=ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate; Xref=Rhea:RHEA:14513, Rhea:RHEA-COMP:9674, Rhea:RHEA-COMP:9677, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:78515, ChEBI:CHEBI:78516, ChEBI:CHEBI:456216;.
Subunit Heterotrimer of A, B and C subunits.
Similarity Belongs to the GatB/GatE family. GatB subfamily.

Keywords [?]

ATP-binding
Ligase
Nucleotide-binding
Protein biosynthesis

Gene Ontology [?]

GO:0050567; Molecular function: glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity.
GO:0006412; Biological process: translation.

Cross-references [?]

Pfam PF02934; GatB_N; 1;
PF02637; GatB_Yqey; 1;
TIGRFAMs TIGR00133; GatB; 1;
PROSITE PS01234; GATB; 1;

Additional information [?]

Size range 449-517 amino acids
Related rules MF_00588 (GATE)
Fusion Nter: MF_00126 (glnS); Cter: None


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