HAMAP rule MF_00144

General rule information [?]

Accession	MF_00144
Dates	1-JUN-2001 (Created) 19-NOV-2022 (Last updated, Version 35)

Name	tRNA_thiouridyl_MnmA

Scope

Bacteria

Templates

P25745 (MNMA_ECOLI); Q97T38 (MNMA_STRPN): [Recover all]

Triggered by

HAMAP; MF_00144 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

MNMA

Protein name

RecName:		Full=tRNA-specific 2-thiouridylase MnmA;
		EC 2.8.1.13;

Gene name

mnmA

Comments [?]

case <OC:Enterobacterales>

Function	Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s(2)U34, the first step of tRNA-mnm(5)s(2)U34 synthesis. Sulfur is provided by IscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs.
Subunit	Interacts with TusE.

else

Function

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34.

end case

Catalytic activity	RHEA:47032: AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[protein] EC 2.8.1.13
Subcellular location	Cytoplasm.
Similarity	Belongs to the MnmA/TRMU family.

Keywords [?]

ATP-binding
Cytoplasm
Nucleotide-binding
RNA-binding
Transferase
tRNA processing
tRNA-binding

Gene Ontology [?]

GO:0016740; Molecular function: transferase activity.
GO:0006400; Biological process: tRNA modification.
GO:0005737; Cellular component: cytoplasm.

Keywords [?]

case <FTTag:disulf>

Disulfide bond

end case

Cross-references [?]

Pfam	PF03054; tRNA_Me_trans; 1;
TIGRFAMs	TIGR00420; TrmU; 1;

Features [?]

From: MNMA_ECOLI (P25745)

Key From To Description Tag Condition FTGroup

BINDING 11 18 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 [GAL]-x-S-G-x-x-[DE]-S

REGION (Optional) 97 99 Interaction with target base in tRNA N-P-D

REGION (Optional) 149 151 Interaction with tRNA [KR]-[DEN]-Q

REGION (Optional) 311 312 Interaction with tRNA [RK]-Y

ACT_SITE 102 102 Nucleophile C

ACT_SITE 199 199 Cysteine persulfide intermediate C

BINDING (Optional) 37 37 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 [MLFI]

BINDING (Optional) 127 127 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 G

SITE (Optional) 128 128 Interaction with tRNA H

SITE (Optional) 344 344 Interaction with tRNA Q

DISULFID 102 199 Alternate disulf C-x*-C

Additional information [?]

Size range	338-431 amino acids
Related rules	None
Fusion	Nter: <nifU>, <ribF>; Cter: None
Comments	Shorter N-terminus in THEM4 and in the second copy of SYNAS; sequences not included in alignment and not taken into account in size range.

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