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HAMAP rule MF_00144

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General rule information [?]

Accession MF_00144
Dates 1-JUN-2001 (Created)
19-NOV-2022 (Last updated, Version 35)
Name tRNA_thiouridyl_MnmA
Templates P25745 (MNMA_ECOLI); Q97T38 (MNMA_STRPN): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=tRNA-specific 2-thiouridylase MnmA;
Gene name

Comments [?]

case <OC:Enterobacterales>
Function Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s(2)U34, the first step of tRNA-mnm(5)s(2)U34 synthesis. Sulfur is provided by IscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs.
Subunit Interacts with TusE.
Function Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34.
end case
Catalytic activity RHEA:47032: AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[protein]
Subcellular location Cytoplasm.
Similarity Belongs to the MnmA/TRMU family.

Keywords [?]

Gene Ontology [?]

GO:0016740; Molecular function: transferase activity.
GO:0006400; Biological process: tRNA modification.
GO:0005737; Cellular component: cytoplasm.

Keywords [?]

case <FTTag:disulf>
end case

Cross-references [?]

Pfam PF03054; tRNA_Me_trans; 1;
TIGRFAMs TIGR00420; TrmU; 1;

Features [?]

From: MNMA_ECOLI (P25745)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     11     18       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     [GAL]-x-S-G-x-x-[DE]-S  
REGION (Optional)     97     99       Interaction with target base in tRNA     N-P-D  
REGION (Optional)     149     151       Interaction with tRNA     [KR]-[DEN]-Q  
REGION (Optional)     311     312       Interaction with tRNA     [RK]-Y  
ACT_SITE     102     102       Nucleophile     C  
ACT_SITE     199     199       Cysteine persulfide intermediate     C  
BINDING (Optional)     37     37       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     [MLFI]  
BINDING (Optional)     127     127       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     G  
SITE (Optional)     128     128       Interaction with tRNA     H  
SITE (Optional)     344     344       Interaction with tRNA     Q  
DISULFID     102     199       Alternate   disulf   C-x*-C  

Additional information [?]

Size range 338-431 amino acids
Related rules None
Fusion Nter: <nifU>, <ribF>; Cter: None
Comments Shorter N-terminus in THEM4 and in the second copy of SYNAS; sequences not included in alignment and not taken into account in size range.