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Annotation rule MF_00144 |
General rule information
[?]
Accession |
MF_00144 |
Dates |
1-JUN-2001 (Created) 14-MAY-2022 (Last updated, Version 34) |
Name |
tRNA_thiouridyl_MnmA |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Protein name |
RecName:
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Full=tRNA-specific 2-thiouridylase MnmA;
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EC 2.8.1.13;
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case <OC:Enterobacterales>
Function |
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s(2)U34, the first step of tRNA-mnm(5)s(2)U34 synthesis. Sulfur is provided by IscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs. |
Subunit |
Interacts with TusE. |
else
Function |
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. |
end case
Catalytic activity |
RHEA:47032: AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[protein]
EC 2.8.1.13
|
Subcellular location |
Cytoplasm. |
Similarity |
Belongs to the MnmA/TRMU family. |
case <FTTag:disulf>
end case
From: MNMA_ECOLI (P25745) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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BINDING
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11
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18
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/ligand="ATP" /ligand_id="30616
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[GAL]-x-S-G-x-x-[DE]-S
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REGION (Optional)
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97
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99
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Interaction with target base in tRNA
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|
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N-P-D
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REGION (Optional)
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149
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|
151
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Interaction with tRNA
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[KR]-[DEN]-Q
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REGION (Optional)
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311
|
|
312
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Interaction with tRNA
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[RK]-Y
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ACT_SITE
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102
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102
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Nucleophile
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C
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ACT_SITE
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199
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199
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Cysteine persulfide intermediate
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C
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BINDING (Optional)
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37
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37
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/ligand="ATP" /ligand_id="30616
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[MLFI]
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BINDING (Optional)
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127
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127
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/ligand="ATP" /ligand_id="30616
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G
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SITE (Optional)
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128
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128
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Interaction with tRNA
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H
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SITE (Optional)
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344
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344
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Interaction with tRNA
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Q
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DISULFID
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102
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199
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Alternate
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disulf
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C-x*-C
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Additional information
[?]
Size range |
338-431 amino acids |
Related rules |
None |
Fusion |
Nter: <nifU>, <ribF>; Cter: None |
Comments |
Shorter N-terminus in THEM4 and in the second copy of SYNAS; sequences not included in alignment and not taken into account in size range. |