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HAMAP rule MF_00144

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General rule information [?]

Accession MF_00144
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 36)
Name tRNA_thiouridyl_MnmA
Scope(s) Bacteria
Template(s) P25745 (MNMA_ECOLI); Q97T38 (MNMA_STRPN); [ Recover all ]
Triggered by HAMAP; MF_00144 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier MNMA
Protein name RecName: Full=tRNA-specific 2-thiouridylase MnmA;
Gene name Name=mnmA;

Comments [?]

case <OC:Enterobacterales>
FUNCTIONCatalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s(2)U34, the first step of tRNA-mnm(5)s(2)U34 synthesis. Sulfur is provided by IscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs.
SUBUNITInteracts with TusE.
FUNCTIONCatalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34.
end case
CATALYTIC ACTIVITY Reaction=AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L- cysteinyl-[protein]; Xref=Rhea:RHEA:47032, Rhea:RHEA-COMP:10131, Rhea:RHEA-COMP:11726, Rhea:RHEA-COMP:11727, Rhea:RHEA-COMP:11728, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17499, ChEBI:CHEBI:29950, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:61963, ChEBI:CHEBI:65315, ChEBI:CHEBI:87170, ChEBI:CHEBI:456215; EC=;
SIMILARITYBelongs to the MnmA/TRMU family.

Keywords [?]

Gene Ontology [?]

GO:0016740; Molecular function:transferase activity
GO:0006400; Biological process:tRNA modification
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF03054; tRNA_Me_trans; 1;
NCBIfam TIGR00420; TrmU; 1;

Features [?]

From: MNMA_ECOLI (P25745)
Key From To Description Tag Condition FTGroup
BINDING 11 18 /ligand="ATP"
REGION 97 99 /note="Interaction with target base in tRNA" N-P-D
REGION 149 151 /note="Interaction with tRNA" [KR]-[DEN]-Q
REGION 311 312 /note="Interaction with tRNA" [RK]-Y
ACT_SITE 102 102 /note="Nucleophile" C
ACT_SITE 199 199 /note="Cysteine persulfide intermediate" C
BINDING 37 37 /ligand="ATP"
BINDING 127 127 /ligand="ATP"
SITE 128 128 /note="Interaction with tRNA" H
SITE 344 344 /note="Interaction with tRNA" Q
DISULFID 102 199 /note="Alternate" disulf C-x*-C

Additional information [?]

Size range 338-431 amino acids
Related rules None
Fusion Nter: <nifU>; <ribF> Cter: None
Comments Shorter N-terminus in THEM4 and in the second copy of SYNAS; sequences not included in alignment and not taken into account in size range.

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