HAMAP rule MF_00144
General rule information
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Accession | MF_00144 |
Dates | 1-JUN-2001 (Created) 19-NOV-2022 (Last updated, Version 35) |
Name | tRNA_thiouridyl_MnmA |
Scope | Bacteria |
Templates | P25745 (MNMA_ECOLI); Q97T38 (MNMA_STRPN): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
|
Protein name |
|
Gene name |
|
Comments
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case <OC:Enterobacterales>
Function | Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s(2)U34, the first step of tRNA-mnm(5)s(2)U34 synthesis. Sulfur is provided by IscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs. |
Subunit | Interacts with TusE. |
else
Function | Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. |
end case
Catalytic activity | RHEA:47032: AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[protein]
EC 2.8.1.13 |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the MnmA/TRMU family. |
Keywords
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Gene Ontology
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GO:0016740; Molecular function: transferase activity.
GO:0006400; Biological process: tRNA modification.
GO:0005737; Cellular component: cytoplasm.
GO:0006400; Biological process: tRNA modification.
GO:0005737; Cellular component: cytoplasm.
Keywords
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case <FTTag:disulf>
end case
Cross-references
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Features
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From: MNMA_ECOLI (P25745) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 11 | 18 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | [GAL]-x-S-G-x-x-[DE]-S | ||||||||
REGION (Optional) | 97 | 99 | Interaction with target base in tRNA | N-P-D | ||||||||
REGION (Optional) | 149 | 151 | Interaction with tRNA | [KR]-[DEN]-Q | ||||||||
REGION (Optional) | 311 | 312 | Interaction with tRNA | [RK]-Y | ||||||||
ACT_SITE | 102 | 102 | Nucleophile | C | ||||||||
ACT_SITE | 199 | 199 | Cysteine persulfide intermediate | C | ||||||||
BINDING (Optional) | 37 | 37 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | [MLFI] | ||||||||
BINDING (Optional) | 127 | 127 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | G | ||||||||
SITE (Optional) | 128 | 128 | Interaction with tRNA | H | ||||||||
SITE (Optional) | 344 | 344 | Interaction with tRNA | Q | ||||||||
DISULFID | 102 | 199 | Alternate | disulf | C-x*-C |
Additional information
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Size range | 338-431 amino acids |
Related rules | None |
Fusion | Nter: <nifU>, <ribF>; Cter: None |
Comments | Shorter N-terminus in THEM4 and in the second copy of SYNAS; sequences not included in alignment and not taken into account in size range. |