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HAMAP rule MF_00146

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General rule information [?]

Accession MF_00146
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 25)
Name dCTP_deaminase
Scope(s) Bacteria
Template(s) P28248 (DCD_ECOLI); Q57872 (DCDB_METJA); P9WP17 (DCDB_MYCTU); Q9KFV3 (DCDB_HALH5); [ Recover all ]
Triggered by HAMAP; MF_00146 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTTag:bifunc1> and <FTTag:bifunc2>
Identifier DCDB
Protein name RecName: Full=dCTP deaminase, dUMP-forming;
AltName: Full=Bifunctional dCTP deaminase:dUTPase;
AltName: Full=DCD-DUT;
Identifier DCD
Protein name RecName: Full=dCTP deaminase;
AltName: Full=Deoxycytidine triphosphate deaminase;
end case
Gene name Name=dcd;

Comments [?]

case <FTTag:bifunc1> and <FTTag:bifunc2>
FUNCTIONBifunctional enzyme that catalyzes both the deamination of dCTP to dUTP and the hydrolysis of dUTP to dUMP without releasing the toxic dUTP intermediate.
CATALYTIC ACTIVITY Reaction=dCTP + 2 H2O = diphosphate + dUMP + NH4(+); Xref=Rhea:RHEA:19205, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:33019, ChEBI:CHEBI:61481, ChEBI:CHEBI:246422; EC=;
PATHWAYPyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP: step 1/1.
FUNCTIONCatalyzes the deamination of dCTP to dUTP.
CATALYTIC ACTIVITY Reaction=dCTP + H(+) + H2O = dUTP + NH4(+); Xref=Rhea:RHEA:22680, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, ChEBI:CHEBI:61481, ChEBI:CHEBI:61555; EC=;
PATHWAYPyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 1/2.
end case
SIMILARITYBelongs to the dCTP deaminase family.

Keywords [?]

Gene Ontology [?]

case <FTTag:bifunc1> and <FTTag:bifunc2>
GO:0033973; Molecular function:dCTP deaminase (dUMP-forming) activity
GO:0006226; Biological process:dUMP biosynthetic process
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0008829; Molecular function:dCTP deaminase activity
GO:0006229; Biological process:dUTP biosynthetic process
end case

Cross-references [?]

Pfam PF00692; dUTPase; 1;
NCBIfam TIGR02274; dCTP_deam; 1;

Features [?]

Key From To Description Tag Condition FTGroup
BINDING 101 106 /ligand="dCTP"
BINDING 127 129 /ligand="dCTP"
ACT_SITE 129 129 /note="Proton donor/acceptor" E
BINDING 119 119 /ligand="dCTP"
BINDING 148 148 /ligand="dCTP"
bifunc1 Q
BINDING 162 162 /ligand="dCTP"
BINDING 170 170 /ligand="dCTP"
BINDING 174 174 /ligand="dCTP"
SITE 116 117 /note="Important for bifunctional activity" bifunc2 G-[FWY]

Additional information [?]

Size range 154-240 amino acids
Related rules MF_00635
Fusion Nter: None Cter: None
Comments Known bifunctional enzymes contain a Gly in position 116 (M.tuberculosis numbering), a Phe/Trp in position 117 and a Gln in position 148 (see PubMed:15539408 and PubMed:18164314).

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