HAMAP rule MF_00146
General rule information
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| Accession | MF_00146 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 25) |
| Name | dCTP_deaminase |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P28248 (DCD_ECOLI); Q57872 (DCDB_METJA); P9WP17 (DCDB_MYCTU); Q9KFV3 (DCDB_HALH5); [ Recover all ] |
| Triggered by |
HAMAP; MF_00146 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| case <FTTag:bifunc1> and <FTTag:bifunc2> | |
| Identifier | DCDB |
| Protein name | RecName: Full=dCTP deaminase, dUMP-forming; EC=3.5.4.30; AltName: Full=Bifunctional dCTP deaminase:dUTPase; AltName: Full=DCD-DUT; |
| else | |
| Identifier | DCD |
| Protein name | RecName: Full=dCTP deaminase; EC=3.5.4.13; AltName: Full=Deoxycytidine triphosphate deaminase; |
| end case | |
| Gene name | Name=dcd; |
Comments
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| case <FTTag:bifunc1> and <FTTag:bifunc2> | |
| FUNCTION | Bifunctional enzyme that catalyzes both the deamination of dCTP to dUTP and the hydrolysis of dUTP to dUMP without releasing the toxic dUTP intermediate. |
| CATALYTIC ACTIVITY | Reaction=dCTP + 2 H2O = diphosphate + dUMP + NH4(+); Xref=Rhea:RHEA:19205, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:33019, ChEBI:CHEBI:61481, ChEBI:CHEBI:246422; EC=3.5.4.30; |
| PATHWAY | Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP: step 1/1. |
| else | |
| FUNCTION | Catalyzes the deamination of dCTP to dUTP. |
| CATALYTIC ACTIVITY | Reaction=dCTP + H(+) + H2O = dUTP + NH4(+); Xref=Rhea:RHEA:22680, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, ChEBI:CHEBI:61481, ChEBI:CHEBI:61555; EC=3.5.4.13; |
| PATHWAY | Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 1/2. |
| end case | |
| SUBUNIT | Homotrimer. |
| SIMILARITY | Belongs to the dCTP deaminase family. |
Keywords
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Gene Ontology
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| case <FTTag:bifunc1> and <FTTag:bifunc2> | |
| GO:0033973; Molecular function:dCTP deaminase (dUMP-forming) activity | |
| GO:0006226; Biological process:dUMP biosynthetic process | |
| else; https://www.ebi.ac.uk/QuickGO/term/else | |
| GO:0008829; Molecular function:dCTP deaminase activity | |
| GO:0006229; Biological process:dUTP biosynthetic process | |
| end case | |
Cross-references
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Features
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| From: DCDB_MYCTU (P9WP17) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 101 | 106 | /ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481" |
[KR]-S-[ST]-x-[GA]-R | ||||||||
| BINDING | 127 | 129 | /ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481" |
[TV]-L-E | ||||||||
| ACT_SITE | 129 | 129 | /note="Proton donor/acceptor" | E | ||||||||
| BINDING | 119 | 119 | /ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481" |
D | ||||||||
| BINDING | 148 | 148 | /ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481" |
bifunc1 | Q | |||||||
| BINDING | 162 | 162 | /ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481" |
Y | ||||||||
| BINDING | 170 | 170 | /ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481" |
K | ||||||||
| BINDING | 174 | 174 | /ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481" |
Q | ||||||||
| SITE | 116 | 117 | /note="Important for bifunctional activity" | bifunc2 | G-[FWY] | |||||||
Additional information
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| Size range | 154-240 amino acids |
| Related rules |
MF_00635 |
| Fusion | Nter: None Cter: None |
| Comments | Known bifunctional enzymes contain a Gly in position 116 (M.tuberculosis numbering), a Phe/Trp in position 117 and a Gln in position 148 (see PubMed:15539408 and PubMed:18164314). |