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Annotation rule MF_00146 |
General rule information
[?]
Accession |
MF_00146 |
Dates |
1-JUN-2001 (Created) 19-NOV-2022 (Last updated, Version 24) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
case <FTTag:bifunc1> and <FTTag:bifunc2>
Protein name |
RecName:
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Full=dCTP deaminase, dUMP-forming;
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EC 3.5.4.30;
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AltName:
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Full=Bifunctional dCTP deaminase:dUTPase;
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AltName:
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Full=DCD-DUT;
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|
else
Protein name |
RecName:
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Full=dCTP deaminase;
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EC 3.5.4.13;
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AltName:
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Full=Deoxycytidine triphosphate deaminase;
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|
end case
case <FTTag:bifunc1> and <FTTag:bifunc2>
Function |
Bifunctional enzyme that catalyzes both the deamination of dCTP to dUTP and the hydrolysis of dUTP to dUMP without releasing the toxic dUTP intermediate. |
Catalytic activity |
RHEA:19205: dCTP + 2 H2O = diphosphate + dUMP + NH4(+)
EC 3.5.4.30
|
Pathway |
Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP: step 1/1. |
else
Function |
Catalyzes the deamination of dCTP to dUTP. |
Catalytic activity |
RHEA:22680: dCTP + H(+) + H2O = dUTP + NH4(+)
EC 3.5.4.13
|
Pathway |
Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 1/2. |
end case
Subunit |
Homotrimer. |
Similarity |
Belongs to the dCTP deaminase family. |
case <FTTag:bifunc1> and <FTTag:bifunc2>
GO:0033973; Molecular function: dCTP deaminase (dUMP-forming) activity.
GO:0006226; Biological process: dUMP biosynthetic process.
else
GO:0008829; Molecular function: dCTP deaminase activity.
GO:0006229; Biological process: dUTP biosynthetic process.
end case
From: DCDB_MYCTU (P9WP17) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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BINDING (Optional)
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101
|
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106
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/ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481
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[KR]-S-[ST]-x-[GA]-R
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BINDING (Optional)
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127
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129
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/ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481
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[TV]-L-E
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ACT_SITE
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129
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129
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Proton donor/acceptor
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E
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BINDING (Optional)
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119
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119
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/ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481
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D
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BINDING (Optional)
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148
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148
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/ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481
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bifunc1
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Q
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BINDING (Optional)
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162
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162
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/ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481
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Y
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BINDING (Optional)
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170
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170
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/ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481
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K
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BINDING (Optional)
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174
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174
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/ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481
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Q
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SITE (Optional)
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116
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117
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Important for bifunctional activity
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bifunc2
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G-[FWY]
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Additional information
[?]
Size range |
154-240 amino acids |
Related rules |
MF_00635 (DUT) |
Fusion |
None |
Comments |
Known bifunctional enzymes contain a Gly in position 116 (M.tuberculosis numbering), a Phe/Trp in position 117 and a Gln in position 148 (see PubMed:15539408 and PubMed:18164314). |