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HAMAP rule MF_00146

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General rule information [?]

Accession MF_00146
Dates 1-JUN-2001 (Created)
19-NOV-2022 (Last updated, Version 24)
Name dCTP_deaminase
Scope
Bacteria
Archaea
Templates P28248 (DCD_ECOLI); Q57872 (DCDB_METJA); P9WP17 (DCDB_MYCTU); Q9KFV3 (DCDB_HALH5): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

case <FTTag:bifunc1> and <FTTag:bifunc2>
Identifier
DCDB
Protein name
RecName: Full=dCTP deaminase, dUMP-forming;
EC 3.5.4.30;
AltName: Full=Bifunctional dCTP deaminase:dUTPase;
AltName: Full=DCD-DUT;
else
Identifier
DCD
Protein name
RecName: Full=dCTP deaminase;
EC 3.5.4.13;
AltName: Full=Deoxycytidine triphosphate deaminase;
end case
Gene name
dcd

Comments [?]

case <FTTag:bifunc1> and <FTTag:bifunc2>
Function Bifunctional enzyme that catalyzes both the deamination of dCTP to dUTP and the hydrolysis of dUTP to dUMP without releasing the toxic dUTP intermediate.
Catalytic activity RHEA:19205: dCTP + 2 H2O = diphosphate + dUMP + NH4(+)
EC 3.5.4.30
Pathway Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP: step 1/1.
else
Function Catalyzes the deamination of dCTP to dUTP.
Catalytic activity RHEA:22680: dCTP + H(+) + H2O = dUTP + NH4(+)
EC 3.5.4.13
Pathway Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 1/2.
end case
Subunit Homotrimer.
Similarity Belongs to the dCTP deaminase family.

Keywords [?]

Hydrolase
Nucleotide metabolism
Nucleotide-binding

Gene Ontology [?]

case <FTTag:bifunc1> and <FTTag:bifunc2>
GO:0033973; Molecular function: dCTP deaminase (dUMP-forming) activity.
GO:0006226; Biological process: dUMP biosynthetic process.
else
GO:0008829; Molecular function: dCTP deaminase activity.
GO:0006229; Biological process: dUTP biosynthetic process.
end case

Cross-references [?]

Pfam PF00692; dUTPase; 1;
TIGRFAMs TIGR02274; dCTP_deam; 1;

Features [?]

From: DCDB_MYCTU (P9WP17)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING (Optional)     101     106       /ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481     [KR]-S-[ST]-x-[GA]-R  
BINDING (Optional)     127     129       /ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481     [TV]-L-E  
ACT_SITE     129     129       Proton donor/acceptor     E  
BINDING (Optional)     119     119       /ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481     D  
BINDING (Optional)     148     148       /ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481   bifunc1   Q  
BINDING (Optional)     162     162       /ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481     Y  
BINDING (Optional)     170     170       /ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481     K  
BINDING (Optional)     174     174       /ligand="dCTP" /ligand_id="ChEBI:CHEBI:61481     Q  
SITE (Optional)     116     117       Important for bifunctional activity   bifunc2   G-[FWY]  

Additional information [?]

Size range 154-240 amino acids
Related rules MF_00635 (DUT)
Fusion None
Comments Known bifunctional enzymes contain a Gly in position 116 (M.tuberculosis numbering), a Phe/Trp in position 117 and a Gln in position 148 (see PubMed:15539408 and PubMed:18164314).


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