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Annotation rule MF_00156 |
General rule information
[?]
Accession |
MF_00156 |
Dates |
1-JUN-2001 (Created) 18-NOV-2019 (Last updated, Version 38) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Protein name |
RecName:
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Full=3-methyl-2-oxobutanoate hydroxymethyltransferase;
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EC 2.1.2.11;
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AltName:
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Full=Ketopantoate hydroxymethyltransferase;
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Short=KPHMT;
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Function |
Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate. |
Catalytic activity |
RHEA:11824: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = (6S)-5,6,7,8-tetrahydrofolate + 2-dehydropantoate
EC 2.1.2.11
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Cofactor |
Mg(2+) Note: Binds 1 Mg(2+) ion per subunit. |
case <OC:Bacteria>
Pathway |
Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. |
else case <OC:Archaea>
Pathway |
Cofactor biosynthesis; coenzyme A biosynthesis. |
end case
Subunit |
Homodecamer; pentamer of dimers. |
Subcellular location |
Cytoplasm. |
Similarity |
Belongs to the PanB family. |
case <OC:Bacteria>
else case <OC:Archaea>
end case
GO:0003864; Molecular function: 3-methyl-2-oxobutanoate hydroxymethyltransferase activity.
case <OC:Bacteria>
GO:0015940; Biological process: pantothenate biosynthetic process.
else case <OC:Archaea>
GO:0015937; Biological process: coenzyme A biosynthetic process.
end case
From: PANB_ECOLI (P31057) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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REGION
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45
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46
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Alpha-ketoisovalerate binding
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ACT_SITE
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181
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181
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Proton acceptor
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E
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METAL
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45
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45
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Magnesium
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D
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METAL
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84
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84
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Magnesium
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D
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METAL
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114
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114
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Magnesium
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E
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BINDING
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84
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84
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Alpha-ketoisovalerate
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D
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BINDING
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112
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112
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Alpha-ketoisovalerate
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K
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Additional information
[?]
Size range |
257-326 amino acids |
Related rules |
None |
Fusion |
None |
Comments |
Most archaea utilize a modified route for coenzyme A biosynthesis. PoK and PPS replace PS and PanK (used by bacteria and eukaryotes) in the conversion of pantoate to 4'-phosphopantothenate. See PubMed=31337720. |