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Annotation rule MF_00156
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General rule information [?]

Accession MF_00156
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 38)
Name PanB
Scope
Bacteria
Archaea
Templates P9WIL7 (PANB_MYCTU); P31057 (PANB_ECOLI); Q9X712 (PANB_CORGL); Q9JZW6 (PANB_NEIMB): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PANB
Protein name
RecName: Full=3-methyl-2-oxobutanoate hydroxymethyltransferase;
EC 2.1.2.11;
AltName: Full=Ketopantoate hydroxymethyltransferase;
Short=KPHMT;
Gene name
panB

Comments [?]

Function Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.
Catalytic activity RHEA:11824: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = (6S)-5,6,7,8-tetrahydrofolate + 2-dehydropantoate
EC 2.1.2.11
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
case <OC:Bacteria>
Pathway Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2.
else case <OC:Archaea>
Pathway Cofactor biosynthesis; coenzyme A biosynthesis.
end case
Subunit Homodecamer; pentamer of dimers.
Subcellular location Cytoplasm.
Similarity Belongs to the PanB family.

Keywords [?]

Cytoplasm
Magnesium
Metal-binding
case <OC:Bacteria>
Pantothenate biosynthesis
else case <OC:Archaea>
Coenzyme A biosynthesis
end case
Transferase

Gene Ontology [?]

GO:0003864; Molecular function: 3-methyl-2-oxobutanoate hydroxymethyltransferase activity.
case <OC:Bacteria>
GO:0015940; Biological process: pantothenate biosynthetic process.
else case <OC:Archaea>
GO:0015937; Biological process: coenzyme A biosynthetic process.
end case
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF02548; Pantoate_transf; 1;
PRINTS PR01415; ANKYRIN; 1;
TIGRFAMs TIGR00222; PanB; 1;

Features [?]

From: PANB_ECOLI (P31057)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     45     46       Alpha-ketoisovalerate binding        
ACT_SITE     181     181       Proton acceptor     E  
METAL     45     45       Magnesium     D  
METAL     84     84       Magnesium     D  
METAL     114     114       Magnesium     E  
BINDING     84     84       Alpha-ketoisovalerate     D  
BINDING     112     112       Alpha-ketoisovalerate     K  

Additional information [?]

Size range 257-326 amino acids
Related rules None
Fusion None
Comments Most archaea utilize a modified route for coenzyme A biosynthesis. PoK and PPS replace PS and PanK (used by bacteria and eukaryotes) in the conversion of pantoate to 4'-phosphopantothenate. See PubMed=31337720.


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