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HAMAP rule MF_00156

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General rule information [?]

Accession MF_00156
Dates 1-JUN-2001 (Created)
28-NOV-2023 (Last updated, Version 43)
Name PanB
Scope(s) Bacteria
Template(s) P9WIL7 (PANB_MYCTU); P31057 (PANB_ECOLI); Q9X712 (PANB_CORGL); Q9JZW6 (PANB_NEIMB); [ Recover all ]
Triggered by HAMAP; MF_00156 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PANB
Protein name RecName: Full=3-methyl-2-oxobutanoate hydroxymethyltransferase;
AltName: Full=Ketopantoate hydroxymethyltransferase;
Gene name Name=panB;

Comments [?]

FUNCTIONCatalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha- ketoisovalerate to form ketopantoate.
CATALYTIC ACTIVITY Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2- oxobutanoate + H2O = (6S)-5,6,7,8-tetrahydrofolate + 2- dehydropantoate; Xref=Rhea:RHEA:11824, ChEBI:CHEBI:11561, ChEBI:CHEBI:11851, ChEBI:CHEBI:15377, ChEBI:CHEBI:15636, ChEBI:CHEBI:57453; EC=;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
case <OC:Bacteria>
PATHWAYCofactor biosynthesis; (R)-pantothenate biosynthesis; (R)- pantoate from 3-methyl-2-oxobutanoate: step 1/2.
else case <OC:Archaea>
PATHWAYCofactor biosynthesis; coenzyme A biosynthesis.
end case
SUBUNITHomodecamer; pentamer of dimers.
SIMILARITYBelongs to the PanB family.

Keywords [?]

Gene Ontology [?]

GO:0003864; Molecular function:3-methyl-2-oxobutanoate hydroxymethyltransferase activity
case <OCellular component:Bacteria>
GO:0015940; Biological process:pantothenate biosynthetic process
else case <OCellular component:Archaea>
GO:0015937; Biological process:coenzyme A biosynthetic process
end case
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF02548; Pantoate_transf; 1;
NCBIfam TIGR00222; PanB; 1;

Features [?]

From: PANB_ECOLI (P31057)
Key From To Description Tag Condition FTGroup
BINDING 45 46 /ligand="3-methyl-2-oxobutanoate"
ACT_SITE 181 181 /note="Proton acceptor" E
BINDING 45 45 /ligand="Mg(2+)"
BINDING 84 84 /ligand="Mg(2+)"
BINDING 114 114 /ligand="Mg(2+)"
BINDING 84 84 /ligand="3-methyl-2-oxobutanoate"
BINDING 112 112 /ligand="3-methyl-2-oxobutanoate"

Additional information [?]

Size range 257-326 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Most archaea utilize a modified route for coenzyme A biosynthesis. PoK and PPS replace PS and PanK (used by bacteria and eukaryotes) in the conversion of pantoate to 4'-phosphopantothenate. See PubMed=31337720.

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