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Annotation rule MF_00157 |
General rule information
[?]
Accession |
MF_00157 |
Dates |
1-JUN-2001 (Created) 18-NOV-2019 (Last updated, Version 21) |
Propagated annotation
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Identifier, protein and gene names
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Protein name |
RecName:
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Full=Ribonuclease T;
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EC 3.1.13.-;
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AltName:
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Full=Exoribonuclease T;
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Short=RNase T;
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Function |
Trims short 3' overhangs of a variety of RNA species, leaving a one or two nucleotide 3' overhang. Responsible for the end-turnover of tRNA: specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis. |
case <FTGroup:1>
Cofactor |
Mg(2+) Note: Binds two Mg(2+) per subunit. The active form of the enzyme binds two Mg(2+) ions in its active site. The first Mg(2+) forms only one salt bridge with the protein. |
end case
Subunit |
Homodimer. |
Similarity |
Belongs to the RNase T family. |
case <FTGroup:1>
end case
GO:0016896; Molecular function: exoribonuclease activity, producing 5'-phosphomonoesters.
case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
From: RNT_ECOLI (P30014) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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ACT_SITE
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181
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181
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Proton donor/acceptor
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H
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METAL
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23
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23
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Magnesium 1; catalytic
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D
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1
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METAL
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23
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23
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Magnesium 2; catalytic
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D
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1
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METAL
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25
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25
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Magnesium 2; catalytic
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E
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1
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METAL
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181
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181
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Magnesium 2; catalytic
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H
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1
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METAL
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186
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186
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Magnesium 2; catalytic
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D
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1
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SITE (Optional)
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29
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29
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Important for substrate binding and specificity
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F
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SITE (Optional)
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77
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77
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Important for substrate binding and specificity
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F
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SITE (Optional)
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124
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124
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Important for substrate binding and specificity
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F
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SITE (Optional)
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146
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146
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Important for substrate binding and specificity
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F
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Additional information
[?]
Size range |
190-275 amino acids |
Related rules |
None |
Fusion |
None |