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HAMAP rule MF_00157

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General rule information [?]

Accession MF_00157
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 25)
Name RNase_T
Scope
Bacteria
Template P30014 (RNT_ECOLI)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
RNT
Protein name
RecName: Full=Ribonuclease T;
EC 3.1.13.-;
AltName: Full=Exoribonuclease T;
Short=RNase T;
Gene name
rnt

Comments [?]

Function Trims short 3' overhangs of a variety of RNA species, leaving a one or two nucleotide 3' overhang. Responsible for the end-turnover of tRNA: specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis.
case <FTGroup:1>
Cofactor Mg(2+)
Note: Binds two Mg(2+) per subunit. The active form of the enzyme binds two Mg(2+) ions in its active site. The first Mg(2+) forms only one salt bridge with the protein.
end case
Subunit Homodimer.
Similarity Belongs to the RNase T family.

Keywords [?]

Exonuclease
Hydrolase
Nuclease
tRNA processing
case <FTGroup:1>
Magnesium
Metal-binding
end case

Gene Ontology [?]

GO:0016896; Molecular function: RNA exonuclease activity, producing 5'-phosphomonoesters.
case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
GO:0006396; Biological process: RNA processing.

Cross-references [?]

Pfam PF00929; RNase_T; 1; trigger=Yes;
NCBIfam TIGR01298; RNaseT; 1;

Features [?]

From: RNT_ECOLI (P30014)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     181     181       Proton donor/acceptor     H  
BINDING     23     23       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" /ligand_note="catalytic     D   1
BINDING     23     23       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" /ligand_note="catalytic     D   1
BINDING     25     25       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" /ligand_note="catalytic     E   1
BINDING     181     181       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" /ligand_note="catalytic     H   1
BINDING     186     186       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" /ligand_note="catalytic     D   1
SITE (Optional)     29     29       Important for substrate binding and specificity     F  
SITE (Optional)     77     77       Important for substrate binding and specificity     F  
SITE (Optional)     124     124       Important for substrate binding and specificity     F  
SITE (Optional)     146     146       Important for substrate binding and specificity     F  

Additional information [?]

Size range 190-275 amino acids
Related rules None
Fusion None


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