HAMAP rule MF_00163

General rule information [?]

Accession	MF_00163
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 31)

Name	Pep_deformylase

Scope

Bacteria

Templates

P0A6K3 (DEF_ECOLI); O31410 (DEF2_GEOSE); Q93LE9 (DEF_LEPIN); P68826 (DEF_STAAU); Q45495 (DEF2_BACSU); P43522 (DEF_THETH): [Recover all]

Triggered by

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

DEF

Protein name

RecName:		Full=Peptide deformylase;
		Short=PDF;
		EC 3.5.1.88;
AltName:		Full=Polypeptide deformylase;

Gene name

def

Comments [?]

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.
Catalytic activity	RHEA:24420: H2O + N-terminal N-formyl-L-methionyl-[peptide] = formate + N-terminal L-methionyl-[peptide] EC 3.5.1.88

case <FTGroup:1>

Cofactor

Fe(2+)
Note: Binds 1 Fe(2+) ion.

end case

Similarity

Belongs to the polypeptide deformylase family.

Keywords [?]

case <FTGroup:1>

end case

Gene Ontology [?]

GO:0042586; Molecular function: peptide deformylase activity.
GO:0006412; Biological process: translation.

Cross-references [?]

Features [?]

From: DEF_ECOLI (P0A6K3)

Key From To Description Tag Condition FTGroup

ACT_SITE 134 134 E

BINDING 91 91 /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 C 1

BINDING 133 133 /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 H 1

BINDING 137 137 /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 H 1

Additional information [?]

Size range	136-232 amino acids
Related rules	None
Fusion	None
Comments	Possible wrong start in a number of bacteria. Def-like proteins exist a number of bacteria and lack some active site and metal-binding residues.