HAMAP rule MF_00163
General rule information
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Accession | MF_00163 |
Dates | 1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 31) |
Name | Pep_deformylase |
Scope | Bacteria |
Templates | P0A6K3 (DEF_ECOLI); O31410 (DEF2_GEOSE); Q93LE9 (DEF_LEPIN); P68826 (DEF_STAAU); Q45495 (DEF2_BACSU); P43522 (DEF_THETH): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions. |
Catalytic activity | RHEA:24420: H2O + N-terminal N-formyl-L-methionyl-[peptide] = formate + N-terminal L-methionyl-[peptide]
EC 3.5.1.88 |
case <FTGroup:1>
Cofactor | Fe(2+) Note: Binds 1 Fe(2+) ion. |
end case
Similarity | Belongs to the polypeptide deformylase family. |
Keywords
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case <FTGroup:1>
end case
Gene Ontology
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GO:0042586; Molecular function: peptide deformylase activity.
GO:0006412; Biological process: translation.
GO:0006412; Biological process: translation.
Cross-references
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Pfam | PF01327; Pep_deformylase; 1; |
PRINTS | PR01576; PDEFORMYLASE; 1; |
NCBIfam | TIGR00079; Pept_deformyl; 1; |
Features
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From: DEF_ECOLI (P0A6K3) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 134 | 134 | E | |||||||||
BINDING | 91 | 91 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 | C | 1 | |||||||
BINDING | 133 | 133 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 | H | 1 | |||||||
BINDING | 137 | 137 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 | H | 1 |
Additional information
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Size range | 136-232 amino acids |
Related rules | None |
Fusion | None |
Comments | Possible wrong start in a number of bacteria. Def-like proteins exist a number of bacteria and lack some active site and metal-binding residues. |