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HAMAP rule MF_00163

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General rule information [?]

Accession MF_00163
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 31)
Name Pep_deformylase
Scope
Bacteria
Templates P0A6K3 (DEF_ECOLI); O31410 (DEF2_GEOSE); Q93LE9 (DEF_LEPIN); P68826 (DEF_STAAU); Q45495 (DEF2_BACSU); P43522 (DEF_THETH): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
DEF
Protein name
RecName: Full=Peptide deformylase;
Short=PDF;
EC 3.5.1.88;
AltName: Full=Polypeptide deformylase;
Gene name
def

Comments [?]

Function Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.
Catalytic activity RHEA:24420: H2O + N-terminal N-formyl-L-methionyl-[peptide] = formate + N-terminal L-methionyl-[peptide]
EC 3.5.1.88
case <FTGroup:1>
Cofactor Fe(2+)
Note: Binds 1 Fe(2+) ion.
end case
Similarity Belongs to the polypeptide deformylase family.

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

GO:0042586; Molecular function: peptide deformylase activity.
GO:0006412; Biological process: translation.

Cross-references [?]

Pfam PF01327; Pep_deformylase; 1;
PRINTS PR01576; PDEFORMYLASE; 1;
NCBIfam TIGR00079; Pept_deformyl; 1;

Features [?]

From: DEF_ECOLI (P0A6K3)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     134     134             E  
BINDING     91     91       /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875     C   1
BINDING     133     133       /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875     H   1
BINDING     137     137       /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875     H   1

Additional information [?]

Size range 136-232 amino acids
Related rules None
Fusion None
Comments Possible wrong start in a number of bacteria. Def-like proteins exist a number of bacteria and lack some active site and metal-binding residues.