HAMAP rule MF_00176

General rule information [?]

Accession	MF_00176
Dates	1-JUN-2001 (Created) 19-NOV-2022 (Last updated, Version 36)

Name	Ser_tRNA_synth_type1

Scope

Bacteria

Archaea

Templates

P0A8L1 (SYS_ECOLI); P34945 (SYS_THET2); Q46F20 (SYS_METBF); P95689 (SYS_STAA8); P54221 (SYS_HALMA): [Recover all]

Triggered by

HAMAP; MF_00176 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

SYS

Protein name

RecName:		Full=Serine--tRNA ligase;
		EC 6.1.1.11;
AltName:		Full=Seryl-tRNA synthetase;
		Short=SerRS;
AltName:		Full=Seryl-tRNA(Ser/Sec) synthetase;

Gene name

serS

Comments [?]

Function	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).
Catalytic activity	RHEA:12292: ATP + L-serine + tRNA(Ser) = AMP + diphosphate + H(+) + L-seryl-tRNA(Ser) EC 6.1.1.11
	RHEA:42580: ATP + L-serine + tRNA(Sec) = AMP + diphosphate + H(+) + L-seryl-tRNA(Sec) EC 6.1.1.11
Pathway	Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.
Subunit	Homodimer. The tRNA molecule binds across the dimer.
Subcellular location	Cytoplasm.
Domain	Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.
Similarity	Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Keywords [?]

Cytoplasm
Aminoacyl-tRNA synthetase
ATP-binding
Ligase
Nucleotide-binding
Protein biosynthesis

Gene Ontology [?]

GO:0004828; Molecular function: serine-tRNA ligase activity.
GO:0005524; Molecular function: ATP binding.
GO:0016260; Biological process: selenocysteine biosynthetic process.
GO:0006434; Biological process: seryl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE	PS50862; AA_TRNA_LIGASE_II; 1;
Pfam	PF02403; Seryl_tRNA_N; 1;
	PF00587; tRNA-synt_2b; 1;
PRINTS	PR00981; TRNASYNTHSER; 1;
TIGRFAMs	TIGR00414; SerS; 1;

Features [?]

From: SYS_THET2 (P34945)

Key From To Description Tag Condition FTGroup

BINDING 256 258 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 R-x-E

BINDING 345 348 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 E-x-x-S

BINDING 225 227 /ligand="L-serine" /ligand_id="ChEBI:CHEBI:33384 T-x-E

BINDING (Optional) 272 272 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 V

BINDING 279 279 /ligand="L-serine" /ligand_id="ChEBI:CHEBI:33384 E

BINDING 380 380 /ligand="L-serine" /ligand_id="ChEBI:CHEBI:33384 [TS]

Additional information [?]

Size range	411-482 amino acids
Related rules	None
Fusion	None
Comments	There are two distinct types of seryl-tRNA synthetase, as differentiated by primary sequence analysis, three-dimensional structure and substrate recognition mechanism: type 1 (MF_00176) is found in the majority of organisms (prokaryotes, eukaryotes and archaea) whereas type 2 (MF_01278) is confined to some methanogenic archaea. METBF (Methanosarcina barkeri) possesses two seryl-tRNA synthetases, one of each type.

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