HAMAP rule MF_00176
General rule information
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Accession | MF_00176 |
Dates | 1-JUN-2001 (Created) 19-NOV-2022 (Last updated, Version 36) |
Name | Ser_tRNA_synth_type1 |
Scope | Bacteria
Archaea |
Templates | P0A8L1 (SYS_ECOLI); P34945 (SYS_THET2); Q46F20 (SYS_METBF); P95689 (SYS_STAA8); P54221 (SYS_HALMA): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). |
Catalytic activity | RHEA:12292: ATP + L-serine + tRNA(Ser) = AMP + diphosphate + H(+) + L-seryl-tRNA(Ser)
EC 6.1.1.11 |
RHEA:42580: ATP + L-serine + tRNA(Sec) = AMP + diphosphate + H(+) + L-seryl-tRNA(Sec)
EC 6.1.1.11 |
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Pathway | Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. |
Subunit | Homodimer. The tRNA molecule binds across the dimer. |
Subcellular location | Cytoplasm. |
Domain | Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding. |
Similarity | Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily. |
Keywords
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Gene Ontology
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GO:0004828; Molecular function: serine-tRNA ligase activity.
GO:0005524; Molecular function: ATP binding.
GO:0016260; Biological process: selenocysteine biosynthetic process.
GO:0006434; Biological process: seryl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
GO:0005524; Molecular function: ATP binding.
GO:0016260; Biological process: selenocysteine biosynthetic process.
GO:0006434; Biological process: seryl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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PROSITE | PS50862; AA_TRNA_LIGASE_II; 1; |
Pfam | PF02403; Seryl_tRNA_N; 1; |
PF00587; tRNA-synt_2b; 1; | |
PRINTS | PR00981; TRNASYNTHSER; 1; |
TIGRFAMs | TIGR00414; SerS; 1; |
Features
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From: SYS_THET2 (P34945) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 256 | 258 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | R-x-E | ||||||||
BINDING | 345 | 348 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | E-x-x-S | ||||||||
BINDING | 225 | 227 | /ligand="L-serine" /ligand_id="ChEBI:CHEBI:33384 | T-x-E | ||||||||
BINDING (Optional) | 272 | 272 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | V | ||||||||
BINDING | 279 | 279 | /ligand="L-serine" /ligand_id="ChEBI:CHEBI:33384 | E | ||||||||
BINDING | 380 | 380 | /ligand="L-serine" /ligand_id="ChEBI:CHEBI:33384 | [TS] |
Additional information
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Size range | 411-482 amino acids |
Related rules | None |
Fusion | None |
Comments | There are two distinct types of seryl-tRNA synthetase, as differentiated by primary sequence analysis, three-dimensional structure and substrate recognition mechanism: type 1 (MF_00176) is found in the majority of organisms (prokaryotes, eukaryotes and archaea) whereas type 2 (MF_01278) is confined to some methanogenic archaea. METBF (Methanosarcina barkeri) possesses two seryl-tRNA synthetases, one of each type. |