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HAMAP rule MF_00176

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General rule information [?]

Accession MF_00176
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 37)
Name Ser_tRNA_synth_type1
Scope(s) Bacteria
Archaea
Template(s) P0A8L1 (SYS_ECOLI); P34945 (SYS_THET2); Q46F20 (SYS_METBF); P95689 (SYS_STAA8); P54221 (SYS_HALMA); [ Recover all ]
Triggered by HAMAP; MF_00176 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SYS
Protein name RecName: Full=Serine--tRNA ligase;
                 EC=6.1.1.11;
AltName: Full=Seryl-tRNA synthetase;
                 Short=SerRS;
AltName: Full=Seryl-tRNA(Ser/Sec) synthetase;
Gene name Name=serS;

Comments [?]

FUNCTIONCatalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec).
CATALYTIC ACTIVITY Reaction=ATP + L-serine + tRNA(Ser) = AMP + diphosphate + H(+) + L- seryl-tRNA(Ser); Xref=Rhea:RHEA:12292, Rhea:RHEA-COMP:9669, Rhea:RHEA-COMP:9703, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:33384, ChEBI:CHEBI:78442, ChEBI:CHEBI:78533, ChEBI:CHEBI:456215; EC=6.1.1.11;
CATALYTIC ACTIVITY Reaction=ATP + L-serine + tRNA(Sec) = AMP + diphosphate + H(+) + L- seryl-tRNA(Sec); Xref=Rhea:RHEA:42580, Rhea:RHEA-COMP:9742, Rhea:RHEA-COMP:10128, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:33384, ChEBI:CHEBI:78442, ChEBI:CHEBI:78533, ChEBI:CHEBI:456215; EC=6.1.1.11;
PATHWAYAminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.
SUBUNITHomodimer. The tRNA molecule binds across the dimer.
SUBCELLULAR LOCATIONCytoplasm.
DOMAINConsists of two distinct domains, a catalytic core and a N- terminal extension that is involved in tRNA binding.
SIMILARITYBelongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Keywords [?]

Cytoplasm
Aminoacyl-tRNA synthetase
ATP-binding
Ligase
Nucleotide-binding
Protein biosynthesis

Gene Ontology [?]

GO:0004828; Molecular function:serine-tRNA ligase activity
GO:0005524; Molecular function:ATP binding
GO:0016260; Biological process:selenocysteine biosynthetic process
GO:0006434; Biological process:seryl-tRNA aminoacylation
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

PROSITE PS50862; AA_TRNA_LIGASE_II; 1;
Pfam PF02403; Seryl_tRNA_N; 1;
Pfam PF00587; tRNA-synt_2b; 1;
PRINTS PR00981; TRNASYNTHSER; 1;
NCBIfam TIGR00414; SerS; 1;

Features [?]

From: SYS_THET2 (P34945)
Key From To Description Tag Condition FTGroup
BINDING 256 258 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 R-x-E
BINDING 345 348 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 E-x-x-S
BINDING 225 227 /ligand="L-serine"
/ligand_id="ChEBI:CHEBI:33384"		 T-x-E
BINDING 272 272 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 V
BINDING 279 279 /ligand="L-serine"
/ligand_id="ChEBI:CHEBI:33384"		 E
BINDING 380 380 /ligand="L-serine"
/ligand_id="ChEBI:CHEBI:33384"		 [TS]

Additional information [?]

Size range 411-482 amino acids
Related rules None
Fusion Nter: None Cter: None



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