HAMAP rule MF_00176
General rule information
[?]
| PURL | https://purl.expasy.org/hamap/rule/MF_00176 |
| Accession | MF_00176 |
| Dates | 28-FEB-2005 (Created)
02-SEP-2024 (Last updated, Version 26) |
| Name | Ser_tRNA_synth_type1 |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P0A8L1; P34945; Q46F20; P95689; P54221; [ Recover all ] |
| Triggered by |
HAMAP; MF_00176 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | SYS |
| Protein name | RecName: Full=Serine--tRNA ligase; EC=6.1.1.11; AltName: Full=Seryl-tRNA synthetase; Short=SerRS; AltName: Full=Seryl-tRNA(Ser/Sec) synthetase; |
| Gene name | Name=serS; |
Comments
[?]
| FUNCTION | Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec). |
| CATALYTIC ACTIVITY | Reaction=tRNA(Ser) + L-serine + ATP = L-seryl-tRNA(Ser) + AMP + diphosphate + H(+); Xref=Rhea:RHEA:12292, Rhea:RHEA-COMP:9669, Rhea:RHEA-COMP:9703, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:33384, ChEBI:CHEBI:78442, ChEBI:CHEBI:78533, ChEBI:CHEBI:456215; EC=6.1.1.11; |
| CATALYTIC ACTIVITY | Reaction=tRNA(Sec) + L-serine + ATP = L-seryl-tRNA(Sec) + AMP + diphosphate + H(+); Xref=Rhea:RHEA:42580, Rhea:RHEA-COMP:9742, Rhea:RHEA-COMP:10128, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:33384, ChEBI:CHEBI:78442, ChEBI:CHEBI:78533, ChEBI:CHEBI:456215; EC=6.1.1.11; |
| PATHWAY | Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. |
| SUBUNIT | Homodimer. The tRNA molecule binds across the dimer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| DOMAIN | Consists of two distinct domains, a catalytic core and a N- terminal extension that is involved in tRNA binding. |
| SIMILARITY | Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily. |
Keywords
[?]
Gene Ontology
[?]
| GO:0004828; Molecular function:serine-tRNA ligase activity |
| GO:0005524; Molecular function:ATP binding |
| GO:0016260; Biological process:selenocysteine biosynthetic process |
| GO:0006434; Biological process:seryl-tRNA aminoacylation |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
[?]
| PROSITE | PS50862; AA_TRNA_LIGASE_II; 1; |
| Pfam | PF02403; Seryl_tRNA_N; 1; |
| Pfam | PF00587; tRNA-synt_2b; 1; |
| PRINTS | PR00981; TRNASYNTHSER; 1; |
| NCBIfam | TIGR00414; SerS; 1; |
Features
[?]
| From: SYS_THET2 (P34945) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 256 | 258 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
R-x-E | ||||||||
| BINDING | 345 | 348 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
E-x-x-S | ||||||||
| BINDING | 225 | 227 | /ligand="L-serine" /ligand_id="ChEBI:CHEBI:33384" |
T-x-E | ||||||||
| BINDING | 272 | 272 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
V | ||||||||
| BINDING | 279 | 279 | /ligand="L-serine" /ligand_id="ChEBI:CHEBI:33384" |
E | ||||||||
| BINDING | 380 | 380 | /ligand="L-serine" /ligand_id="ChEBI:CHEBI:33384" |
[TS] | ||||||||
Additional information
[?]
| Size range | 411-482 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |