HAMAP logo
Due to maintenance work, this service will be unavailable from Mon Nov 11 17:30 until Tue Nov 12 09:00 CET. Apologies for the inconvenience.

HAMAP rule MF_00178

Send feedback

General rule information [?]

Accession MF_00178
Dates 1-JUN-2001 (Created)
14-MAY-2024 (Last updated, Version 38)
Name Lumazine_synth
Scope(s) Bacteria
Archaea
Template(s) P61714 (RISB_ECOLI); O66529 (RISB_AQUAE); P11998 (RISB_BACSU); P61711 (RISB2_BRUAB); P9WHE9 (RISB_MYCTU); Q81MB5 (RISB_BACAN); Q57DY1 (RISB1_BRUAB); Q986N2 (RISB2_RHILO); Q983B0 (RISB1_RHILO); Q8YGH2 (RISB1_BRUME); Q57751 (RISB_METJA); P66038 (RISB_SALTY); Q2YKV1 (RISB2_BRUA2); Q2YNC6 (RISB1_BRUA2); [ Recover all ]
Triggered by HAMAP; MF_00178 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RISB
Protein name RecName: Full=6,7-dimethyl-8-ribityllumazine synthase;
                 Short=DMRL synthase;
                 Short=Lumazine synthase;
                 Short=LS;
                 EC=2.5.1.78;
Gene name Name=ribH;

Comments [?]

FUNCTIONCatalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2- butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.
CATALYTIC ACTIVITY Reaction=(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D- ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2 H2O + phosphate; Xref=Rhea:RHEA:26152, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15934, ChEBI:CHEBI:43474, ChEBI:CHEBI:58201, ChEBI:CHEBI:58830; EC=2.5.1.78;
PATHWAYCofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D- ribitylamino)uracil: step 1/2.
case <OC:Aquificia> or <OC:Bacillales> or <OC:Gammaproteobacteria> or <OC:Methanococci>
SUBUNITForms an icosahedral capsid composed of 60 subunits, arranged as a dodecamer of pentamers.
else case <OC:Mycobacterium>
SUBUNITHomopentamer.
end case
SIMILARITYBelongs to the DMRL synthase family.

Keywords [?]

Riboflavin biosynthesis
Transferase

Gene Ontology [?]

GO:0000906; Molecular function:6,7-dimethyl-8-ribityllumazine synthase activity
GO:0009231; Biological process:riboflavin biosynthetic process

Cross-references [?]

Pfam PF00885; DMRL_synthase; 1;
NCBIfam TIGR00114; Lumazine-synth; 1;

Features [?]

From: RISB_AQUAE (O66529)
Key From To Description Tag Condition FTGroup
BINDING 56 58 /ligand="5-amino-6-(D-ribitylamino)uracil"
/ligand_id="ChEBI:CHEBI:15934"
BINDING 80 82 /ligand="5-amino-6-(D-ribitylamino)uracil"
/ligand_id="ChEBI:CHEBI:15934"
BINDING 85 86 /ligand="(2S)-2-hydroxy-3-oxobutyl phosphate"
/ligand_id="ChEBI:CHEBI:58830"		 [AQSGEDKTN]-[TS]
ACT_SITE 88 88 /note="Proton donor" [HR]
BINDING 22 22 /ligand="5-amino-6-(D-ribitylamino)uracil"
/ligand_id="ChEBI:CHEBI:15934"		 [FWY]
BINDING 113 113 /ligand="5-amino-6-(D-ribitylamino)uracil"
/ligand_id="ChEBI:CHEBI:15934"		 [FNLSDMYIHV]
BINDING 127 127 /ligand="(2S)-2-hydroxy-3-oxobutyl phosphate"
/ligand_id="ChEBI:CHEBI:58830"		 [RH]

Additional information [?]

Size range 133-190 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Lumazine synthase structures are either observed as pentamers, dimers of pentamers or dodecamers of pentamers.



View rule in raw text format (no links)