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Annotation rule MF_00178
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General rule information [?]

Accession MF_00178
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 33)
Name Lumazine_synth
Scope
Bacteria
Archaea
Templates P61714 (RISB_ECOLI); O66529 (RISB_AQUAE); P11998 (RISB_BACSU); P61711 (RISB2_BRUAB); P9WHE9 (RISB_MYCTU); Q81MB5 (RISB_BACAN); Q57DY1 (RISB1_BRUAB); Q986N2 (RISB2_RHILO); Q983B0 (RISB1_RHILO); Q8YGH2 (RISB1_BRUME); Q57751 (RISB_METJA); P66038 (RISB_SALTY); Q2YKV1 (RISB2_BRUA2); Q2YNC6 (RISB1_BRUA2): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
RISB
Protein name
RecName: Full=6,7-dimethyl-8-ribityllumazine synthase;
Short=DMRL synthase;
Short=Lumazine synthase;
Short=LS;
EC 2.5.1.78;
Gene name
ribH

Comments [?]

Function Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.
Catalytic activity RHEA:26152: (2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2 H2O + phosphate
EC 2.5.1.78
Pathway Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.
case <OC:Aquificae> or <OC:Bacillales> or <OC:Gammaproteobacteria> or <OC:Methanococci>
Subunit Forms an icosahedral capsid composed of 60 subunits, arranged as a dodecamer of pentamers.
else case <OC:Mycobacterium>
Subunit Homopentamer.
end case
Similarity Belongs to the DMRL synthase family.

Keywords [?]

Riboflavin biosynthesis
Transferase

Gene Ontology [?]

GO:0000906; Molecular function: 6,7-dimethyl-8-ribityllumazine synthase activity.
GO:0009231; Biological process: riboflavin biosynthetic process.

Cross-references [?]

Pfam PF00885; DMRL_synthase; 1;
TIGRFAMs TIGR00114; Lumazine-synth; 1;

Features [?]

From: RISB_AQUAE (O66529)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     56     58       5-amino-6-(D-ribitylamino)uracil binding        
REGION     80     82       5-amino-6-(D-ribitylamino)uracil binding        
REGION (Optional)     85     86       1-deoxy-L-glycero-tetrulose 4-phosphate binding     [AQSGEDKTN]-[TS]  
ACT_SITE     88     88       Proton donor     [HR]  
BINDING     22     22       5-amino-6-(D-ribitylamino)uracil     [FWY]  
BINDING     113     113       5-amino-6-(D-ribitylamino)uracil; via amide nitrogen and carbonyl oxygen     [FNLSDMYIHV]  
BINDING     127     127       1-deoxy-L-glycero-tetrulose 4-phosphate     [RH]  

Additional information [?]

Size range 133-190 amino acids
Related rules None
Fusion None
Comments Lumazine synthase structures are either observed as pentamers, dimers of pentamers or dodecamers of pentamers.


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