HAMAP rule MF_00178

General rule information [?]

Accession	MF_00178
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 37)

Name	Lumazine_synth

Scope

Bacteria

Archaea

Templates

P61714 (RISB_ECOLI); O66529 (RISB_AQUAE); P11998 (RISB_BACSU); P61711 (RISB2_BRUAB); P9WHE9 (RISB_MYCTU); Q81MB5 (RISB_BACAN); Q57DY1 (RISB1_BRUAB); Q986N2 (RISB2_RHILO); Q983B0 (RISB1_RHILO); Q8YGH2 (RISB1_BRUME); Q57751 (RISB_METJA); P66038 (RISB_SALTY); Q2YKV1 (RISB2_BRUA2); Q2YNC6 (RISB1_BRUA2): [Recover all]

Triggered by

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

RISB

Protein name

RecName:		Full=6,7-dimethyl-8-ribityllumazine synthase;
		Short=DMRL synthase;
		Short=Lumazine synthase;
		Short=LS;
		EC 2.5.1.78;

Gene name

ribH

Comments [?]

Function	Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.
Catalytic activity	RHEA:26152: (2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2 H2O + phosphate EC 2.5.1.78
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.

case <OC:Aquificae> or <OC:Bacillales> or <OC:Gammaproteobacteria> or <OC:Methanococci>

Subunit

Forms an icosahedral capsid composed of 60 subunits, arranged as a dodecamer of pentamers.

else case <OC:Mycobacterium>

Subunit

Homopentamer.

end case

Similarity

Belongs to the DMRL synthase family.

Keywords [?]

Gene Ontology [?]

GO:0000906; Molecular function: 6,7-dimethyl-8-ribityllumazine synthase activity.
GO:0009231; Biological process: riboflavin biosynthetic process.

Cross-references [?]

Pfam	PF00885; DMRL_synthase; 1;
NCBIfam	TIGR00114; Lumazine-synth; 1;

Features [?]

From: RISB_AQUAE (O66529)

Key From To Description Tag Condition FTGroup

BINDING 56 58 /ligand="5-amino-6-(D-ribitylamino)uracil" /ligand_id="ChEBI:CHEBI:15934

BINDING 80 82 /ligand="5-amino-6-(D-ribitylamino)uracil" /ligand_id="ChEBI:CHEBI:15934

BINDING (Optional) 85 86 /ligand="(2S)-2-hydroxy-3-oxobutyl phosphate" /ligand_id="ChEBI:CHEBI:58830 [AQSGEDKTN]-[TS]

ACT_SITE 88 88 Proton donor [HR]

BINDING 22 22 /ligand="5-amino-6-(D-ribitylamino)uracil" /ligand_id="ChEBI:CHEBI:15934 [FWY]

BINDING 113 113 /ligand="5-amino-6-(D-ribitylamino)uracil" /ligand_id="ChEBI:CHEBI:15934 [FNLSDMYIHV]

BINDING 127 127 /ligand="(2S)-2-hydroxy-3-oxobutyl phosphate" /ligand_id="ChEBI:CHEBI:58830 [RH]

Additional information [?]

Size range	133-190 amino acids
Related rules	None
Fusion	None
Comments	Lumazine synthase structures are either observed as pentamers, dimers of pentamers or dodecamers of pentamers.