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Annotation rule MF_00178 |
General rule information
[?]
Accession |
MF_00178 |
Dates |
1-JUN-2001 (Created) 18-NOV-2019 (Last updated, Version 33) |
Propagated annotation
[?]
Identifier, protein and gene names
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Protein name |
RecName:
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Full=6,7-dimethyl-8-ribityllumazine synthase;
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Short=DMRL synthase;
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Short=Lumazine synthase;
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Short=LS;
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EC 2.5.1.78;
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Function |
Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin. |
Catalytic activity |
RHEA:26152: (2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2 H2O + phosphate
EC 2.5.1.78
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Pathway |
Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2. |
case <OC:Aquificae> or <OC:Bacillales> or <OC:Gammaproteobacteria> or <OC:Methanococci>
Subunit |
Forms an icosahedral capsid composed of 60 subunits, arranged as a dodecamer of pentamers. |
else case <OC:Mycobacterium>
end case
Similarity |
Belongs to the DMRL synthase family. |
GO:0000906; Molecular function: 6,7-dimethyl-8-ribityllumazine synthase activity.
GO:0009231; Biological process: riboflavin biosynthetic process.
From: RISB_AQUAE (O66529) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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REGION
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56
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58
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5-amino-6-(D-ribitylamino)uracil binding
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REGION
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80
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82
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5-amino-6-(D-ribitylamino)uracil binding
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REGION (Optional)
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85
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86
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1-deoxy-L-glycero-tetrulose 4-phosphate binding
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[AQSGEDKTN]-[TS]
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ACT_SITE
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88
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88
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Proton donor
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[HR]
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BINDING
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22
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22
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5-amino-6-(D-ribitylamino)uracil
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[FWY]
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BINDING
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113
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113
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5-amino-6-(D-ribitylamino)uracil; via amide nitrogen and carbonyl oxygen
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[FNLSDMYIHV]
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BINDING
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127
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127
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1-deoxy-L-glycero-tetrulose 4-phosphate
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[RH]
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Additional information
[?]
Size range |
133-190 amino acids |
Related rules |
None |
Fusion |
None |
Comments |
Lumazine synthase structures are either observed as pentamers, dimers of pentamers or dodecamers of pentamers. |