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HAMAP rule MF_00180

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General rule information [?]

Accession MF_00180
Dates 1-JUN-2001 (Created)
28-NOV-2023 (Last updated, Version 28)
Name RibB
Scope(s) Bacteria
Template(s) P0A7J0 (RIBB_ECOLI); Q60364 (RIBB_METJA); [ Recover all ]
Triggered by HAMAP; MF_00180 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RIBB
Protein name RecName: Full=3,4-dihydroxy-2-butanone 4-phosphate synthase;
                 Short=DHBP synthase;
Gene name Name=ribB;

Comments [?]

FUNCTIONCatalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.
CATALYTIC ACTIVITY Reaction=D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+); Xref=Rhea:RHEA:18457, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740, ChEBI:CHEBI:58121, ChEBI:CHEBI:58830; EC=;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 2 divalent metal cations per subunit. Magnesium or manganese.;
PATHWAYCofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3- oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.
SIMILARITYBelongs to the DHBP synthase family.

Keywords [?]

Gene Ontology [?]

GO:0000287; Molecular function:magnesium ion binding
GO:0008686; Molecular function:3,4-dihydroxy-2-butanone-4-phosphate synthase activity
GO:0030145; Molecular function:manganese ion binding
GO:0009231; Biological process:riboflavin biosynthetic process

Cross-references [?]

Pfam PF00926; DHBP_synthase; 1;
NCBIfam TIGR00506; RibB; 1;

Features [?]

From: RIBB_METJA (Q60364)
Key From To Description Tag Condition FTGroup
BINDING 25 26 /ligand="D-ribulose 5-phosphate"
BINDING 161 165 /ligand="D-ribulose 5-phosphate"
BINDING 26 26 /ligand="Mg(2+)"
BINDING 26 26 /ligand="Mg(2+)"
BINDING 164 164 /ligand="Mg(2+)"
BINDING 30 30 /ligand="D-ribulose 5-phosphate"
SITE 147 147 /note="Essential for catalytic activity" H
SITE 185 185 /note="Essential for catalytic activity" E

Additional information [?]

Size range 200-247 amino acids
Related rules MF_01283
Fusion Nter: None Cter: <ribA-like>
Comments RibA and RibB are fused in some organisms (see MF_01283 for bifunctional RibBA). Some ribB are followed by a ribA-like domain, which does not seem to code for GTP cyclohydrolase II activity because it lacks all the residues important for activity.

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