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Annotation rule MF_00186 |
General rule information
[?]
Accession |
MF_00186 |
Dates |
1-JUN-2001 (Created) 18-NOV-2019 (Last updated, Version 34) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Protein name |
RecName:
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Full=Glycerol kinase;
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EC 2.7.1.30;
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AltName:
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Full=ATP:glycerol 3-phosphotransferase;
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AltName:
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Full=Glycerokinase;
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Short=GK;
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Function |
Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate. |
Catalytic activity |
RHEA:21644: ATP + glycerol = ADP + H(+) + sn-glycerol 3-phosphate
EC 2.7.1.30
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case <OC:Enterobacterales>
Activity regulation |
Activity of this regulatory enzyme is affected by several metabolites. Allosterically and non-competitively inhibited by fructose 1,6-bisphosphate (FBP) and unphosphorylated phosphocarrier protein EIIA-Glc (III-Glc), an integral component of the bacterial phosphotransferase (PTS) system. |
else case <OC:Firmicutes>
Activity regulation |
Activated by phosphorylation and inhibited by fructose 1,6-bisphosphate (FBP). |
else case not <OC:Archaea>
Activity regulation |
Inhibited by fructose 1,6-bisphosphate (FBP). |
end case
Pathway |
Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. |
case <OC:Enterobacterales>
Subunit |
Homotetramer and homodimer (in equilibrium). Heterodimer with EIIA-Glc. Binds 1 zinc ion per glycerol kinase EIIA-Glc dimer. The zinc ion is important for dimerization. |
else case <OC:Firmicutes>
Subunit |
Homotetramer and homodimer (in equilibrium). |
end case
case <FTGroup:1>
Ptm |
The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), including enzyme I, and histidine-containing protein (HPr) are required for the phosphorylation, which leads to the activation of the enzyme. |
end case
Similarity |
Belongs to the FGGY kinase family. |
case <OC:Enterobacterales>
end case
case <FTGroup:1>
end case
GO:0005524; Molecular function: ATP binding.
GO:0004370; Molecular function: glycerol kinase activity.
GO:0006072; Biological process: glycerol-3-phosphate metabolic process.
From: GLPK_ECOLI (P0A6F3) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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NP_BIND
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14
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16
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ATP
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NP_BIND
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412
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416
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ATP
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REGION
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84
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85
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Substrate binding
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R-[EK]
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case <OC:Enterobacterales>
REGION
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234
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236
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Allosteric FBP inhibitor binding
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G-G-T
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end case
REGION
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246
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247
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Substrate binding
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D-Q
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case <OC:Enterobacterales>
METAL
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479
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479
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Zinc; shared with EIIA-Glc
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E
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end case
BINDING
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14
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14
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Substrate
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[TS]
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BINDING
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18
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18
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ATP
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[RK]
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BINDING
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136
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136
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Substrate
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Y
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BINDING
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268
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268
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ATP
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T
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BINDING
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311
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311
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ATP; via carbonyl oxygen
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G
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BINDING
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315
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315
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ATP; via amide nitrogen
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Q
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BINDING
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330
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330
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ATP
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[TS]
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case <OC:Bacilli>
From: GLPK_ENTCA (O34153) |
MOD_RES
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232
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232
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Phosphohistidine; by HPr
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H
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1
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end case
Additional information
[?]
Size range |
490-517 amino acids |
Related rules |
None |
Fusion |
None |
Comments |
Possible wrong start in RHOBA; sequence not included in alignment and not taken into account in size range. There is a possible divergent glpK in ARCFU: AF0866. |