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Annotation rule MF_00186
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General rule information [?]

Accession MF_00186
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 34)
Name Glycerol_kin
Scope
Bacteria
Archaea
Templates P0A6F3 (GLPK_ECOLI); P18157 (GLPK_BACSU); O34153 (GLPK_ENTCA); O34154 (GLPK_ENTFA); Q9WX53 (GLPK_THEAQ); O66131 (GLPK_THETH); O93623 (GLPK_THEKO): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
GLPK
Protein name
RecName: Full=Glycerol kinase;
EC 2.7.1.30;
AltName: Full=ATP:glycerol 3-phosphotransferase;
AltName: Full=Glycerokinase;
Short=GK;
Gene name
glpK

Comments [?]

Function Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.
Catalytic activity RHEA:21644: ATP + glycerol = ADP + H(+) + sn-glycerol 3-phosphate
EC 2.7.1.30
case <OC:Enterobacterales>
Activity regulation Activity of this regulatory enzyme is affected by several metabolites. Allosterically and non-competitively inhibited by fructose 1,6-bisphosphate (FBP) and unphosphorylated phosphocarrier protein EIIA-Glc (III-Glc), an integral component of the bacterial phosphotransferase (PTS) system.
else case <OC:Firmicutes>
Activity regulation Activated by phosphorylation and inhibited by fructose 1,6-bisphosphate (FBP).
else case not <OC:Archaea>
Activity regulation Inhibited by fructose 1,6-bisphosphate (FBP).
end case
Pathway Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
case <OC:Enterobacterales>
Subunit Homotetramer and homodimer (in equilibrium). Heterodimer with EIIA-Glc. Binds 1 zinc ion per glycerol kinase EIIA-Glc dimer. The zinc ion is important for dimerization.
else case <OC:Firmicutes>
Subunit Homotetramer and homodimer (in equilibrium).
end case
case <FTGroup:1>
Ptm The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), including enzyme I, and histidine-containing protein (HPr) are required for the phosphorylation, which leads to the activation of the enzyme.
end case
Similarity Belongs to the FGGY kinase family.

Keywords [?]

case <OC:Enterobacterales>
Allosteric enzyme
Metal-binding
Zinc
end case
Glycerol metabolism
Transferase
Kinase
ATP-binding
Nucleotide-binding
case <FTGroup:1>
Phosphoprotein
end case

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004370; Molecular function: glycerol kinase activity.
GO:0006072; Biological process: glycerol-3-phosphate metabolic process.

Cross-references [?]

PROSITE PS00933; FGGY_KINASES_1; 1;
PS00445; FGGY_KINASES_2; 1;
Pfam PF02782; FGGY_C; 1;
PF00370; FGGY_N; 1;
TIGRFAMs TIGR01311; Glycerol_kin; 1;

Features [?]

From: GLPK_ECOLI (P0A6F3)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     14     16       ATP        
NP_BIND     412     416       ATP        
REGION     84     85       Substrate binding     R-[EK]  
case <OC:Enterobacterales>
REGION     234     236       Allosteric FBP inhibitor binding     G-G-T  
end case
REGION     246     247       Substrate binding     D-Q  
case <OC:Enterobacterales>
METAL     479     479       Zinc; shared with EIIA-Glc     E  
end case
BINDING     14     14       Substrate     [TS]  
BINDING     18     18       ATP     [RK]  
BINDING     136     136       Substrate     Y  
BINDING     268     268       ATP     T  
BINDING     311     311       ATP; via carbonyl oxygen     G  
BINDING     315     315       ATP; via amide nitrogen     Q  
BINDING     330     330       ATP     [TS]  
case <OC:Bacilli>
From: GLPK_ENTCA (O34153)
MOD_RES     232     232       Phosphohistidine; by HPr     H   1
end case

Additional information [?]

Size range 490-517 amino acids
Related rules None
Fusion None
Comments Possible wrong start in RHOBA; sequence not included in alignment and not taken into account in size range. There is a possible divergent glpK in ARCFU: AF0866.


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