HAMAP rule MF_00186
General rule information
[?]
| Accession | MF_00186 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 40) |
| Name | Glycerol_kin |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P0A6F3 (GLPK_ECOLI); P18157 (GLPK_BACSU); O34153 (GLPK_ENTCA); O34154 (GLPK_ENTFA); Q9WX53 (GLPK_THEAQ); O66131 (GLPK_THETH); O93623 (GLPK_THEKO); [ Recover all ] |
| Triggered by |
HAMAP; MF_00186 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | GLPK |
| Protein name | RecName: Full=Glycerol kinase; EC=2.7.1.30; AltName: Full=ATP:glycerol 3-phosphotransferase; AltName: Full=Glycerokinase; Short=GK; |
| Gene name | Name=glpK; |
Comments
[?]
| FUNCTION | Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn- glycerol 3-phosphate. |
| CATALYTIC ACTIVITY | Reaction=ATP + glycerol = ADP + H(+) + sn-glycerol 3-phosphate; Xref=Rhea:RHEA:21644, ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:30616, ChEBI:CHEBI:57597, ChEBI:CHEBI:456216; EC=2.7.1.30; |
| case <OC:Enterobacterales> | |
| ACTIVITY REGULATION | Activity of this regulatory enzyme is affected by several metabolites. Allosterically and non-competitively inhibited by fructose 1,6-bisphosphate (FBP) and unphosphorylated phosphocarrier protein EIIA-Glc (III-Glc), an integral component of the bacterial phosphotransferase (PTS) system. |
| else case <OC:Bacillota> | |
| ACTIVITY REGULATION | Activated by phosphorylation and inhibited by fructose 1,6-bisphosphate (FBP). |
| else case not <OC:Archaea> | |
| ACTIVITY REGULATION | Inhibited by fructose 1,6-bisphosphate (FBP). |
| end case | |
| PATHWAY | Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. |
| case <OC:Enterobacterales> | |
| SUBUNIT | Homotetramer and homodimer (in equilibrium). Heterodimer with EIIA-Glc. Binds 1 zinc ion per glycerol kinase EIIA-Glc dimer. The zinc ion is important for dimerization. |
| else case <OC:Bacillota> | |
| SUBUNIT | Homotetramer and homodimer (in equilibrium). |
| end case | |
| case <FTGroup:1> | |
| PTM | The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), including enzyme I, and histidine-containing protein (HPr) are required for the phosphorylation, which leads to the activation of the enzyme. |
| end case | |
| SIMILARITY | Belongs to the FGGY kinase family. |
Keywords
[?]
| case <OC:Enterobacterales> | |
| Allosteric enzyme | |
| Metal-binding | |
| Zinc | |
| end case | |
| Glycerol metabolism | |
| Transferase | |
| Kinase | |
| ATP-binding | |
| Nucleotide-binding | |
| case <FTGroup:1> | |
| Phosphoprotein | |
| end case | |
Gene Ontology
[?]
| GO:0005524; Molecular function:ATP binding |
| GO:0004370; Molecular function:glycerol kinase activity |
| GO:0006072; Biological process:glycerol-3-phosphate metabolic process |
Cross-references
[?]
| PROSITE | PS00933; FGGY_KINASES_1; 1; |
| PROSITE | PS00445; FGGY_KINASES_2; 1; |
| Pfam | PF02782; FGGY_C; 1; |
| Pfam | PF00370; FGGY_N; 1; |
| NCBIfam | TIGR01311; Glycerol_kin; 1; |
Features
[?]
| From: GLPK_ECOLI (P0A6F3) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 14 | 16 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
|||||||||
| BINDING | 412 | 416 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
|||||||||
| BINDING | 84 | 85 | /ligand="substrate" | R-[EK] | ||||||||
| BINDING | 246 | 247 | /ligand="substrate" | D-Q | ||||||||
| case <OC:Enterobacterales> | ||||||||||||
| BINDING | 479 | 479 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="ligand shared with EIIA-Glc" |
E | ||||||||
| BINDING | 235 | 235 | /ligand="beta-D-fructose 1,6-bisphosphate" /ligand_id="ChEBI:CHEBI:32966" /ligand_note="allosteric inhibitor" |
G | ||||||||
| BINDING | 237 | 237 | /ligand="beta-D-fructose 1,6-bisphosphate" /ligand_id="ChEBI:CHEBI:32966" /ligand_note="allosteric inhibitor" |
R | ||||||||
| end case | ||||||||||||
| BINDING | 14 | 14 | /ligand="substrate" | [TS] | ||||||||
| BINDING | 18 | 18 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[RK] | ||||||||
| BINDING | 136 | 136 | /ligand="substrate" | Y | ||||||||
| BINDING | 268 | 268 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
T | ||||||||
| BINDING | 311 | 311 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G | ||||||||
| BINDING | 315 | 315 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
Q | ||||||||
| BINDING | 330 | 330 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[TS] | ||||||||
| From: GLPK_ENTCA (O34153) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Bacilli> | ||||||||||||
| MOD_RES | 232 | 232 | /note="Phosphohistidine; by HPr" | H | 1 | |||||||
| end case | ||||||||||||
Additional information
[?]
| Size range | 490-517 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Possible wrong start in RHOBA; sequence not included in alignment and not taken into account in size range. There is a possible divergent glpK in ARCFU: AF0866. |