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HAMAP rule MF_00191

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General rule information [?]

Accession MF_00191
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 48)
Name IspH
Templates P62623 (ISPH_ECOLI); O67625 (ISPH_AQUAE); Q55643 (ISPH_SYNY3): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=4-hydroxy-3-methylbut-2-enyl diphosphate reductase;
Short=HMBPP reductase;
Gene name

Comments [?]

Function Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.
Catalytic activity RHEA:24488: H2O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]
RHEA:24825: dimethylallyl diphosphate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]
case <FTGroup:1>
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster per subunit.
end case
Pathway Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.
case <OC:Enterobacterales>
Subunit Homodimer.
end case
Similarity Belongs to the IspH family.

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
end case
GO:0051745; Molecular function: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity.
GO:0019288; Biological process: isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway.
GO:0016114; Biological process: terpenoid biosynthetic process.
GO:0050992; Biological process: dimethylallyl diphosphate biosynthetic process.

Cross-references [?]

Pfam PF02401; LYTB; 1;
NCBIfam TIGR00216; IspH_lytB; 1;

Features [?]

From: ISPH_ECOLI (P62623)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     225     227       /ligand="substrate     S-x-N  
ACT_SITE     126     126       Proton donor     E  
BINDING     12     12       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883     C   1
BINDING     96     96       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883     C   1
BINDING     197     197       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883     C   1
BINDING     41     41       /ligand="substrate     H  
BINDING (Optional)     74     74       /ligand="substrate     H  
BINDING     124     124       /ligand="substrate     H  
BINDING     167     167       /ligand="substrate     [TS]  
BINDING     269     269       /ligand="substrate     S  

Additional information [?]

Size range 273-417 amino acids
Related rules None
Fusion Nter: None; Cter: <S1>