HAMAP rule MF_00191
General rule information
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| Accession | MF_00191 |
| Dates | 1-JUN-2001 (Created)
28-JUN-2024 (Last updated, Version 50) |
| Name | IspH |
| Scope(s) |
Bacteria |
| Template(s) | P62623 (ISPH_ECOLI); O67625 (ISPH_AQUAE); Q55643 (ISPH_SYNY3); [ Recover all ] |
| Triggered by |
HAMAP; MF_00191 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | ISPH |
| Protein name | RecName: Full=4-hydroxy-3-methylbut-2-enyl diphosphate reductase; Short=HMBPP reductase; EC=1.17.7.4; |
| Gene name | Name=ispH; |
Comments
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| FUNCTION | Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis. |
| CATALYTIC ACTIVITY | Reaction=H2O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]- [ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:24488, Rhea:RHEA- COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:128753, ChEBI:CHEBI:128769; EC=1.17.7.4; |
| CATALYTIC ACTIVITY | Reaction=dimethylallyl diphosphate + H2O + 2 oxidized [2Fe-2S]- [ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:24825, Rhea:RHEA- COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57623, ChEBI:CHEBI:128753; EC=1.17.7.4; |
| case <FTGroup:1> | |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster per subunit.; |
| end case | |
| PATHWAY | Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3- methylbutenyl diphosphate: step 1/1. |
| PATHWAY | Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 6/6. |
| case <OC:Enterobacterales> | |
| SUBUNIT | Homodimer. |
| end case | |
| SIMILARITY | Belongs to the IspH family. |
Keywords
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| case <FTGroup:1> | |
| 4Fe-4S | |
| Iron | |
| Iron-sulfur | |
| Metal-binding | |
| end case | |
| Isoprene biosynthesis | |
| Oxidoreductase | |
Gene Ontology
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| case <FTGroup:1> | |
| GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding | |
| end case | |
| GO:0051745; Molecular function:4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity | |
| GO:0019288; Biological process:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway | |
| GO:0016114; Biological process:terpenoid biosynthetic process | |
| GO:0050992; Biological process:dimethylallyl diphosphate biosynthetic process | |
Cross-references
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Features
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| From: ISPH_ECOLI (P62623) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 126 | 126 | /note="Proton donor" | E | ||||||||
| BINDING | 12 | 12 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
| BINDING | 96 | 96 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
| BINDING | 197 | 197 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
| BINDING | 41 | 41 | /ligand="(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate" /ligand_id="ChEBI:CHEBI:128753" |
H | ||||||||
| BINDING | 41 | 41 | /ligand="dimethylallyl diphosphate" /ligand_id="ChEBI:CHEBI:57623" |
H | ||||||||
| BINDING | 41 | 41 | /ligand="isopentenyl diphosphate" /ligand_id="ChEBI:CHEBI:128769" |
H | ||||||||
| BINDING | 74 | 74 | /ligand="(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate" /ligand_id="ChEBI:CHEBI:128753" |
H | ||||||||
| BINDING | 74 | 74 | /ligand="dimethylallyl diphosphate" /ligand_id="ChEBI:CHEBI:57623" |
H | ||||||||
| BINDING | 74 | 74 | /ligand="isopentenyl diphosphate" /ligand_id="ChEBI:CHEBI:128769" |
H | ||||||||
| BINDING | 124 | 124 | /ligand="(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate" /ligand_id="ChEBI:CHEBI:128753" |
H | ||||||||
| BINDING | 124 | 124 | /ligand="dimethylallyl diphosphate" /ligand_id="ChEBI:CHEBI:57623" |
H | ||||||||
| BINDING | 124 | 124 | /ligand="isopentenyl diphosphate" /ligand_id="ChEBI:CHEBI:128769" |
H | ||||||||
| BINDING | 167 | 167 | /ligand="(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate" /ligand_id="ChEBI:CHEBI:128753" |
[TS] | ||||||||
| BINDING | 225 | 225 | /ligand="(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate" /ligand_id="ChEBI:CHEBI:128753" |
S | ||||||||
| BINDING | 225 | 225 | /ligand="dimethylallyl diphosphate" /ligand_id="ChEBI:CHEBI:57623" |
S | ||||||||
| BINDING | 225 | 225 | /ligand="isopentenyl diphosphate" /ligand_id="ChEBI:CHEBI:128769" |
S | ||||||||
| BINDING | 226 | 226 | /ligand="(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate" /ligand_id="ChEBI:CHEBI:128753" |
S | ||||||||
| BINDING | 226 | 226 | /ligand="dimethylallyl diphosphate" /ligand_id="ChEBI:CHEBI:57623" |
S | ||||||||
| BINDING | 226 | 226 | /ligand="isopentenyl diphosphate" /ligand_id="ChEBI:CHEBI:128769" |
S | ||||||||
| BINDING | 227 | 227 | /ligand="(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate" /ligand_id="ChEBI:CHEBI:128753" |
N | ||||||||
| BINDING | 227 | 227 | /ligand="dimethylallyl diphosphate" /ligand_id="ChEBI:CHEBI:57623" |
N | ||||||||
| BINDING | 227 | 227 | /ligand="isopentenyl diphosphate" /ligand_id="ChEBI:CHEBI:128769" |
N | ||||||||
| BINDING | 269 | 269 | /ligand="(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate" /ligand_id="ChEBI:CHEBI:128753" |
S | ||||||||
| BINDING | 269 | 269 | /ligand="dimethylallyl diphosphate" /ligand_id="ChEBI:CHEBI:57623" |
S | ||||||||
| BINDING | 269 | 269 | /ligand="isopentenyl diphosphate" /ligand_id="ChEBI:CHEBI:128769" |
S | ||||||||
Additional information
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| Size range | 273-417 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: <S1> |