HAMAP rule MF_00191
General rule information
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Accession | MF_00191 |
Dates | 1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 48) |
Name | IspH |
Scope | Bacteria |
Templates | P62623 (ISPH_ECOLI); O67625 (ISPH_AQUAE); Q55643 (ISPH_SYNY3): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis. |
Catalytic activity | RHEA:24488: H2O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]
EC 1.17.7.4 |
RHEA:24825: dimethylallyl diphosphate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]
EC 1.17.7.4 |
case <FTGroup:1>
Cofactor | [4Fe-4S] cluster Note: Binds 1 [4Fe-4S] cluster per subunit. |
end case
Pathway | Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. |
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. |
case <OC:Enterobacterales>
Subunit | Homodimer. |
end case
Similarity | Belongs to the IspH family. |
Keywords
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case <FTGroup:1>
end case
Gene Ontology
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case <FTGroup:1>
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
end case
GO:0051745; Molecular function: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity.
GO:0019288; Biological process: isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway.
GO:0016114; Biological process: terpenoid biosynthetic process.
GO:0050992; Biological process: dimethylallyl diphosphate biosynthetic process.
GO:0019288; Biological process: isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway.
GO:0016114; Biological process: terpenoid biosynthetic process.
GO:0050992; Biological process: dimethylallyl diphosphate biosynthetic process.
Cross-references
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Features
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From: ISPH_ECOLI (P62623) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 225 | 227 | /ligand="substrate | S-x-N | ||||||||
ACT_SITE | 126 | 126 | Proton donor | E | ||||||||
BINDING | 12 | 12 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 | C | 1 | |||||||
BINDING | 96 | 96 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 | C | 1 | |||||||
BINDING | 197 | 197 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 | C | 1 | |||||||
BINDING | 41 | 41 | /ligand="substrate | H | ||||||||
BINDING (Optional) | 74 | 74 | /ligand="substrate | H | ||||||||
BINDING | 124 | 124 | /ligand="substrate | H | ||||||||
BINDING | 167 | 167 | /ligand="substrate | [TS] | ||||||||
BINDING | 269 | 269 | /ligand="substrate | S |
Additional information
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Size range | 273-417 amino acids |
Related rules | None |
Fusion | Nter: None; Cter: <S1> |