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Annotation rule MF_00197 |
General rule information
[?]
Accession |
MF_00197 |
Dates |
1-JUN-2001 (Created) 21-JAN-2020 (Last updated, Version 33) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Protein name |
RecName:
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Full=Diaminopimelate epimerase;
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Short=DAP epimerase;
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EC 5.1.1.7;
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AltName:
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Full=PLP-independent amino acid racemase;
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case <OC:Bacteria>
Function |
Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan. |
end case
case <OC:Archaea>
Function |
Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine. |
end case
Catalytic activity |
RHEA:15393: (2S,6S)-2,6-diaminoheptanedioate = meso-2,6-diaminoheptanedioate
EC 5.1.1.7
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Pathway |
Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1. |
Subunit |
Homodimer. |
Subcellular location |
Cytoplasm. |
Similarity |
Belongs to the diaminopimelate epimerase family. |
GO:0008837; Molecular function: diaminopimelate epimerase activity.
GO:0009089; Biological process: lysine biosynthetic process via diaminopimelate.
GO:0005737; Cellular component: cytoplasm.
From: DAPF_HAEIN (P44859) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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REGION
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74
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|
75
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Substrate binding
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|
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[GI]-N
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REGION
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208
|
|
209
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Substrate binding
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|
|
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E-R
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|
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REGION
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218
|
|
219
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Substrate binding
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|
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G-[ST]
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ACT_SITE
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73
|
|
73
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Proton donor
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C
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ACT_SITE
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217
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217
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Proton acceptor
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C
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BINDING
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|
11
|
|
11
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|
Substrate
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|
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N
|
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BINDING
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|
44
|
|
44
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Substrate
|
|
|
|
Q
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BINDING
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64
|
|
64
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Substrate
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|
|
|
[NQ]
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BINDING
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157
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|
157
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Substrate
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|
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N
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BINDING
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190
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190
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Substrate
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|
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N
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SITE
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159
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159
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Could be important to modulate the pK values of the two catalytic cysteine residues
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H
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SITE
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208
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208
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Could be important to modulate the pK values of the two catalytic cysteine residues
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E
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case <OC:Gammaproteobacteria>
SITE
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268
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268
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Important for dimerization
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[YF]
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end case
Additional information
[?]
Size range |
249-333 amino acids |
Related rules |
None |
Fusion |
None |
Comments |
DcsC (S.lavendulae) belongs to the same group of racemases/epimerases as diaminopimelate epimerase (DapF) and also utilizes a two-base mechanism involving a pair of cysteine residues, however it function as racemase with O-ureido-L-serine as a substrate. |