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Annotation rule MF_00197
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General rule information [?]

Accession MF_00197
Dates 1-JUN-2001 (Created)
21-JAN-2020 (Last updated, Version 33)
Name DAP_epimerase
Scope
Bacteria
Archaea
Templates P0A6K1 (DAPF_ECOLI); P44859 (DAPF_HAEIN); Q8NP73 (DAPF_CORGL); P9WP19 (DAPF_MYCTU): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
DAPF
Protein name
RecName: Full=Diaminopimelate epimerase;
Short=DAP epimerase;
EC 5.1.1.7;
AltName: Full=PLP-independent amino acid racemase;
Gene name
dapF

Comments [?]

case <OC:Bacteria>
Function Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.
end case
case <OC:Archaea>
Function Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine.
end case
Catalytic activity RHEA:15393: (2S,6S)-2,6-diaminoheptanedioate = meso-2,6-diaminoheptanedioate
EC 5.1.1.7
Pathway Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1.
Subunit Homodimer.
Subcellular location Cytoplasm.
Similarity Belongs to the diaminopimelate epimerase family.

Keywords [?]

Cytoplasm
Amino-acid biosynthesis
Isomerase
Lysine biosynthesis

Gene Ontology [?]

GO:0008837; Molecular function: diaminopimelate epimerase activity.
GO:0009089; Biological process: lysine biosynthetic process via diaminopimelate.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF01678; DAP_epimerase; 1;
TIGRFAMs TIGR00652; DapF; 1;
PROSITE PS01326; DAP_EPIMERASE; 1;

Features [?]

From: DAPF_HAEIN (P44859)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     74     75       Substrate binding     [GI]-N  
REGION     208     209       Substrate binding     E-R  
REGION     218     219       Substrate binding     G-[ST]  
ACT_SITE     73     73       Proton donor     C  
ACT_SITE     217     217       Proton acceptor     C  
BINDING     11     11       Substrate     N  
BINDING     44     44       Substrate     Q  
BINDING     64     64       Substrate     [NQ]  
BINDING     157     157       Substrate     N  
BINDING     190     190       Substrate     N  
SITE     159     159       Could be important to modulate the pK values of the two catalytic cysteine residues     H  
SITE     208     208       Could be important to modulate the pK values of the two catalytic cysteine residues     E  
case <OC:Gammaproteobacteria>
SITE     268     268       Important for dimerization     [YF]  
end case

Additional information [?]

Size range 249-333 amino acids
Related rules None
Fusion None
Comments DcsC (S.lavendulae) belongs to the same group of racemases/epimerases as diaminopimelate epimerase (DapF) and also utilizes a two-base mechanism involving a pair of cysteine residues, however it function as racemase with O-ureido-L-serine as a substrate.


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