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HAMAP rule MF_00197

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General rule information [?]

Accession MF_00197
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 36)
Name DAP_epimerase
Scope(s) Bacteria
Archaea
Template(s) P0A6K1 (DAPF_ECOLI); P44859 (DAPF_HAEIN); Q8NP73 (DAPF_CORGL); P9WP19 (DAPF_MYCTU); [ Recover all ]
Triggered by HAMAP; MF_00197 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier DAPF
Protein name RecName: Full=Diaminopimelate epimerase;
                 Short=DAP epimerase;
                 EC=5.1.1.7;
AltName: Full=PLP-independent amino acid racemase;
Gene name Name=dapF;

Comments [?]

case <OC:Bacteria>
FUNCTIONCatalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L- lysine and an essential component of the bacterial peptidoglycan.
end case
case <OC:Archaea>
FUNCTIONCatalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L- lysine.
end case
CATALYTIC ACTIVITY Reaction=(2S,6S)-2,6-diaminoheptanedioate = meso-2,6- diaminoheptanedioate; Xref=Rhea:RHEA:15393, ChEBI:CHEBI:57609, ChEBI:CHEBI:57791; EC=5.1.1.7;
PATHWAYAmino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1.
SUBUNITHomodimer.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the diaminopimelate epimerase family.

Keywords [?]


Gene Ontology [?]

GO:0008837; Molecular function:diaminopimelate epimerase activity
GO:0009089; Biological process:lysine biosynthetic process via diaminopimelate
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF01678; DAP_epimerase; 1;
NCBIfam TIGR00652; DapF; 1;
PROSITE PS01326; DAP_EPIMERASE; 1;

Features [?]

From: DAPF_HAEIN (P44859)
Key From To Description Tag Condition FTGroup
BINDING 74 75 /ligand="substrate" [GI]-N
BINDING 208 209 /ligand="substrate" E-R
BINDING 218 219 /ligand="substrate" G-[ST]
ACT_SITE 73 73 /note="Proton donor" C
ACT_SITE 217 217 /note="Proton acceptor" C
BINDING 11 11 /ligand="substrate" N
BINDING 44 44 /ligand="substrate" Q
BINDING 64 64 /ligand="substrate" [NQ]
BINDING 157 157 /ligand="substrate" N
BINDING 190 190 /ligand="substrate" N
SITE 159 159 /note="Could be important to modulate the pK values of the two catalytic cysteine residues" H
SITE 208 208 /note="Could be important to modulate the pK values of the two catalytic cysteine residues" E
case <OC:Gammaproteobacteria>
SITE 268 268 /note="Important for dimerization" [YF]
end case

Additional information [?]

Size range 249-333 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments DcsC (S.lavendulae) belongs to the same group of racemases/epimerases as diaminopimelate epimerase (DapF) and also utilizes a two-base mechanism involving a pair of cysteine residues, however it function as racemase with O-ureido-L-serine as a substrate.



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