HAMAP rule MF_00202
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00202 |
| Accession | MF_00202 |
| Dates | 1-JUN-2001 (Created)
14-MAY-2024 (Last updated, Version 42) |
| Name | Idi |
| Scope(s) |
Bacteria Actinomycetota Pseudomonadota Archaea Halobacteria |
| Template(s) | Q46822 (IDI_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00202 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | IDI |
| Protein name | RecName: Full=Isopentenyl-diphosphate Delta-isomerase; Short=IPP isomerase; EC=5.3.3.2; AltName: Full=IPP:DMAPP isomerase; AltName: Full=Isopentenyl pyrophosphate isomerase; |
| Gene name | Name=idi; |
Comments
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| FUNCTION | Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP). |
| CATALYTIC ACTIVITY | Reaction=isopentenyl diphosphate = dimethylallyl diphosphate; Xref=Rhea:RHEA:23284, ChEBI:CHEBI:57623, ChEBI:CHEBI:128769; EC=5.3.3.2; |
| case <FT:6> | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit. The magnesium ion binds only when substrate is bound.; |
| end case | |
| case <FTGroup:1> | |
| COFACTOR | Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 1 Mn(2+) ion per subunit.; |
| end case | |
| PATHWAY | Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from isopentenyl diphosphate: step 1/1. |
| case <Property:PHOTOSYN> | |
| PATHWAY | Porphyrin-containing compound metabolism; chlorophyll biosynthesis. |
| end case | |
| case <OC:Enterobacterales> | |
| SUBUNIT | Homodimer. |
| end case | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the IPP isomerase type 1 family. |
Keywords
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| Cytoplasm | |
| Isomerase | |
| Isoprene biosynthesis | |
| case <Property:PHOTOSYN> | |
| Chlorophyll biosynthesis | |
| Photosynthesis | |
| end case | |
| case <FT:6> | |
| Magnesium | |
| end case | |
| case <FTGroup:1> | |
| Manganese | |
| end case | |
| case <FTGroup:1> or <FT:6> | |
| Metal-binding | |
| end case | |
Gene Ontology
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| GO:0004452; Molecular function:isopentenyl-diphosphate delta-isomerase activity | |
| GO:0050992; Biological process:dimethylallyl diphosphate biosynthetic process | |
| case <Property:PHOTOSYN> | |
| GO:0015995; Biological process:chlorophyll biosynthetic process | |
| GO:0015979; Biological process:photosynthesis | |
| end case | |
| GO:0005737; Cellular component:cytoplasm | |
Cross-references
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Features
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| From: IDI_ECOLI (Q46822) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 67 | 67 | C | |||||||||
| ACT_SITE | 116 | 116 | E | |||||||||
| BINDING | 25 | 25 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" |
H | 1 | |||||||
| BINDING | 32 | 32 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" |
H | 1 | |||||||
| BINDING | 69 | 69 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" |
H | 1 | |||||||
| BINDING | 87 | 87 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | ||||||||
| BINDING | 114 | 114 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" |
E | 1 | |||||||
| BINDING | 116 | 116 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" |
E | 1 | |||||||
Additional information
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| Size range | 175-213 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | HALSA and HALMA putative conserved active site cysteine residue is replaced by an alanine. HALSA also possess a type-2 IDI. |