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Annotation rule MF_00206 |
General rule information
[?]
Accession |
MF_00206 |
Dates |
1-JUN-2001 (Created) 18-NOV-2019 (Last updated, Version 52) |
case <OC:Bacteria> or <OC:Archaea>
end case
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Protein name |
RecName:
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Full=Lipoyl synthase;
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EC 2.8.1.8;
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AltName:
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Full=Lip-syn;
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Short=LS;
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AltName:
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Full=Lipoate synthase;
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AltName:
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Full=Lipoic acid synthase;
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AltName:
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Full=Sulfur insertion protein LipA;
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Function |
Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives. |
Catalytic activity |
RHEA:16585: [[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]
EC 2.8.1.8
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Cofactor |
[4Fe-4S] cluster Note: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. |
case <OC:Bacillales>
Pathway |
Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]. |
else
Pathway |
Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. |
end case
Subcellular location |
Cytoplasm. |
Similarity |
Belongs to the radical SAM superfamily. Lipoyl synthase family. |
GO:0016783; Molecular function: sulfurtransferase activity.
GO:0016992; Molecular function: lipoate synthase activity.
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0009107; Biological process: lipoate biosynthetic process.
GO:0009249; Biological process: protein lipoylation.
GO:0005737; Cellular component: cytoplasm.
From: LIPA_MYCTU (P9WK91) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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METAL
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55
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55
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Iron-sulfur 1 (4Fe-4S)
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C
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METAL
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60
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60
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Iron-sulfur 1 (4Fe-4S)
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C
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METAL
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66
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66
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Iron-sulfur 1 (4Fe-4S)
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C
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METAL
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81
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81
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Iron-sulfur 2 (4Fe-4S-S-AdoMet)
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C
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METAL
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85
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85
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Iron-sulfur 2 (4Fe-4S-S-AdoMet)
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C
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METAL
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88
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88
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Iron-sulfur 2 (4Fe-4S-S-AdoMet)
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C
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METAL
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292
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292
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Iron-sulfur 1 (4Fe-4S)
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S
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Additional information
[?]
Size range |
276-373 amino acids |
Related rules |
MF_03128 (LIAS supersedes the current rule); MF_03129 (LISC supersedes the current rule) |
Fusion |
None |
Comments |
E.coli requires only 2 proteins for the endogenous pathway of protein lipoylation, LipB (MF_00013) and LipA (MF_00206), whereas B.subtilis (and probably many Firmicutes) requires 3 enzymes: LipM (MF_02118), LipL (MF_02119) and LipA (MF_00206). |