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Annotation rule MF_00206
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General rule information [?]

Accession MF_00206
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 52)
Name Lipoyl_synth
Scope
Bacteria
Archaea
Templates P60716 (LIPA_ECOLI); O32129 (LIPA_BACSU); P9WK91 (LIPA_MYCTU); Q8DLC2 (LIPA2_THEEB); Q97U63 (LIPA_SACS2): [Recover all]
case <OC:Bacteria> or <OC:Archaea>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
LIPA
Protein name
RecName: Full=Lipoyl synthase;
EC 2.8.1.8;
AltName: Full=Lip-syn;
Short=LS;
AltName: Full=Lipoate synthase;
AltName: Full=Lipoic acid synthase;
AltName: Full=Sulfur insertion protein LipA;
Gene name
lipA

Comments [?]

Function Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.
Catalytic activity RHEA:16585: [[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]
EC 2.8.1.8
Cofactor [4Fe-4S] cluster
Note: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
case <OC:Bacillales>
Pathway Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].
else
Pathway Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.
end case
Subcellular location Cytoplasm.
Similarity Belongs to the radical SAM superfamily. Lipoyl synthase family.

Keywords [?]

4Fe-4S
Cytoplasm
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
Transferase

Gene Ontology [?]

GO:0016783; Molecular function: sulfurtransferase activity.
GO:0016992; Molecular function: lipoate synthase activity.
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0009107; Biological process: lipoate biosynthetic process.
GO:0009249; Biological process: protein lipoylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF04055; Radical_SAM; 1;
TIGRFAMs TIGR00510; LipA; 1;

Features [?]

From: LIPA_MYCTU (P9WK91)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     55     55       Iron-sulfur 1 (4Fe-4S)     C  
METAL     60     60       Iron-sulfur 1 (4Fe-4S)     C  
METAL     66     66       Iron-sulfur 1 (4Fe-4S)     C  
METAL     81     81       Iron-sulfur 2 (4Fe-4S-S-AdoMet)     C  
METAL     85     85       Iron-sulfur 2 (4Fe-4S-S-AdoMet)     C  
METAL     88     88       Iron-sulfur 2 (4Fe-4S-S-AdoMet)     C  
METAL     292     292       Iron-sulfur 1 (4Fe-4S)     S  

Additional information [?]

Size range 276-373 amino acids
Related rules MF_03128 (LIAS supersedes the current rule); MF_03129 (LISC supersedes the current rule)
Fusion None
Comments E.coli requires only 2 proteins for the endogenous pathway of protein lipoylation, LipB (MF_00013) and LipA (MF_00206), whereas B.subtilis (and probably many Firmicutes) requires 3 enzymes: LipM (MF_02118), LipL (MF_02119) and LipA (MF_00206).


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