HAMAP rule MF_00208
General rule information
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Accession | MF_00208 |
Dates | 1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 49) |
Name | MurE |
Scope | Bacteria |
Templates | P22188 (MURE_ECOLI); Q2FZP6 (MURE_STAA8); Q97PS1 (MURE_STRPN); Q9WY79 (MURE_THEMA); Q9A196 (MURE_STRP1): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
|
case <FT:5>
Protein name |
|
else case <FT:1>
Protein name |
|
else
Protein name |
|
end case
Gene name |
|
Comments
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case <FT:5>
Function | Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan. |
Catalytic activity | RHEA:23676: ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate
EC 6.3.2.13 |
else case <FT:1>
Function | Catalyzes the addition of L-lysine to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan. |
Catalytic activity | RHEA:17969: ATP + L-lysine + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-L-lysine
EC 6.3.2.7 |
else
Function | Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan. |
end case
Cofactor | Mg(2+) |
Pathway | Cell wall biogenesis; peptidoglycan biosynthesis. |
Subcellular location | Cytoplasm. |
Ptm | Carboxylation is probably crucial for Mg(2+) binding and, consequently, for the gamma-phosphate positioning of ATP. |
Similarity | Belongs to the MurCDEF family. MurE subfamily. |
Keywords
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ATP-binding
Cell cycle
Cell division
Cell shape
Cell wall biogenesis/degradation
Cytoplasm
Ligase
Magnesium
Nucleotide-binding
Peptidoglycan synthesis
Cell cycle
Cell division
Cell shape
Cell wall biogenesis/degradation
Cytoplasm
Ligase
Magnesium
Nucleotide-binding
Peptidoglycan synthesis
Gene Ontology
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case <FT:5>
GO:0008765; Molecular function: UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity.
else case <FT:1>
GO:0047482; Molecular function: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-L-lysine ligase activity.
else
GO:0016881; Molecular function: acid-amino acid ligase activity.
end case
GO:0009252; Biological process: peptidoglycan biosynthetic process.
GO:0005737; Cellular component: cytoplasm.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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Pfam | PF01225; Mur_ligase; 1; |
PF02875; Mur_ligase_C; 1; | |
PF08245; Mur_ligase_M; 1; | |
NCBIfam | TIGR01085; MurE; 1; |
Features
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From: MURE_STAA8 (Q2FZP6) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
MOTIF (Optional) | 406 | 409 | L-lysine recognition motif | D-[DN]-P-[NA] | ||||||||
From: MURE_ECOLI (P22188) | ||||||||||||
BINDING | 116 | 122 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | G-T-x-G-K-[ST]-[ST] | ||||||||
BINDING (Optional) | 44 | 46 | /ligand="UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate" /ligand_id="ChEBI:CHEBI:83900 | H-[QRK]-[AVCT] | ||||||||
BINDING (Optional) | 158 | 159 | /ligand="UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate" /ligand_id="ChEBI:CHEBI:83900 | [TS]-T | ||||||||
MOTIF (Optional) | 414 | 417 | Meso-diaminopimelate recognition motif | D-N-P-R | ||||||||
BINDING (Optional) | 27 | 27 | /ligand="UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate" /ligand_id="ChEBI:CHEBI:83900 | L | ||||||||
BINDING (Optional) | 29 | 29 | /ligand="UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate" /ligand_id="ChEBI:CHEBI:83900 | [ST] | ||||||||
BINDING (Optional) | 157 | 157 | /ligand="UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate" /ligand_id="ChEBI:CHEBI:83900 | [NQ] | ||||||||
BINDING | 185 | 185 | /ligand="UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate" /ligand_id="ChEBI:CHEBI:83900 | [ST] | ||||||||
BINDING (Optional) | 191 | 191 | /ligand="UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate" /ligand_id="ChEBI:CHEBI:83900 | Q | ||||||||
BINDING | 193 | 193 | /ligand="UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate" /ligand_id="ChEBI:CHEBI:83900 | R | ||||||||
MOD_RES | 225 | 225 | N6-carboxylysine | K |
case <FT:5>
end case
Additional information
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Size range | 427-540 amino acids |
Related rules | MF_00046 (MURC) |
Fusion | None |
Comments | MurE catalyzes the addition of the third amino acid residue of the peptide chain of peptidoglycan. This residue, generally a diamino acid, varies among the bacterial species: meso-diaminopimelic acid (meso-A2pm) for most Gram-negative bacteria and bacilli, L-lysine for most Gram-positive bacteria, L-ornithine, meso-lanthionine, LL-A2pm, L-diaminobutyric acid, L-homoserine, etc. in particular species (PubMed=4568761). MurE is highly specific in its choice of amino acid, to ensure the presence of the specific amino acid at the third position of the pentapeptide, which is required for the lateral cross-linking that is vital for peptidoglycan integrity. MurE from Thermotoga maritima was shown to be able to add both L-lysine and D-lysine that are found in its peptidoglycan. |