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HAMAP rule MF_00222

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General rule information [?]

Accession MF_00222
Dates 1-JUN-2001 (Created)
12-MAR-2024 (Last updated, Version 42)
Name Shikimate_DH_AroE
Scope(s) Bacteria
Template(s) P15770 (AROE_ECOLI); O67049 (AROE_AQUAE); P43876 (AROE_HAEIN); P56119 (AROE_HELPY); Q8Y9N5 (AROE_LISMO); Q5HNV1 (AROE_STAEQ); Q5SJF8 (AROE_THET8); Q9KVT3 (AROE_VIBCH); Q58484 (AROE_METJA); P44774 (SHDHL_HAEIN); Q9X5C9 (AROE_CORGL); [ Recover all ]
Triggered by HAMAP; MF_00222 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier AROE
Protein name RecName: Full=Shikimate dehydrogenase (NADP(+));
Gene name Name=aroE;

Comments [?]

FUNCTIONInvolved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).
CATALYTIC ACTIVITY Reaction=NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH; Xref=Rhea:RHEA:17737, ChEBI:CHEBI:15378, ChEBI:CHEBI:16630, ChEBI:CHEBI:36208, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=;
PATHWAYMetabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.
SIMILARITYBelongs to the shikimate dehydrogenase family.

Keywords [?]

Gene Ontology [?]

GO:0004764; Molecular function:shikimate 3-dehydrogenase (NADP+) activity
GO:0009423; Biological process:chorismate biosynthetic process

Cross-references [?]

Pfam PF01488; Shikimate_DH; 1;
Pfam PF08501; Shikimate_dh_N; 1;
NCBIfam TIGR00507; AroE; 1;

Features [?]

From: AROE_AQUAE (O67049)
Key From To Description Tag Condition FTGroup
BINDING 130 134 /ligand="NADP(+)"
BINDING 153 158 /ligand="NADP(+)"
BINDING 19 21 /ligand="shikimate"
ACT_SITE 70 70 /note="Proton acceptor" K
BINDING 82 82 /ligand="NADP(+)"
BINDING 66 66 /ligand="shikimate"
BINDING 91 91 /ligand="shikimate"
BINDING 106 106 /ligand="shikimate"
BINDING 214 214 /ligand="NADP(+)"
BINDING 216 216 /ligand="shikimate"
BINDING 235 235 /ligand="NADP(+)"
BINDING 242 242 /ligand="shikimate"

Additional information [?]

Size range 253-295 amino acids
Related rules MF_01578
Fusion Nter: MF_00214 (aroD) Cter: None
Comments Aroe in CORGL and CORGB catalyzes the NAD(+)-dependent oxidation of both quinate and shikimate with a higher catalytic efficiency for quinate rather than shikimate, and seems to play a key role in the quinate-degradation pathway (PubMed=18566515).

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