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HAMAP rule MF_00222

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General rule information [?]

Accession MF_00222
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 41)
Name Shikimate_DH_AroE
Templates P15770 (AROE_ECOLI); O67049 (AROE_AQUAE); P43876 (AROE_HAEIN); P56119 (AROE_HELPY); Q8Y9N5 (AROE_LISMO); Q5HNV1 (AROE_STAEQ); Q5SJF8 (AROE_THET8); Q9KVT3 (AROE_VIBCH); Q58484 (AROE_METJA); P44774 (SHDHL_HAEIN); Q9X5C9 (AROE_CORGL): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Shikimate dehydrogenase (NADP(+));
Gene name

Comments [?]

Function Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).
Catalytic activity RHEA:17737: NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH
Pathway Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.
Subunit Homodimer.
Similarity Belongs to the shikimate dehydrogenase family.

Keywords [?]

Gene Ontology [?]

GO:0004764; Molecular function: shikimate 3-dehydrogenase (NADP+) activity.
GO:0009073; Biological process: aromatic amino acid family biosynthetic process.

Cross-references [?]

Pfam PF01488; Shikimate_DH; 1;
PF08501; Shikimate_dh_N; 1;
NCBIfam TIGR00507; AroE; 1;

Features [?]

From: AROE_AQUAE (O67049)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     130     134       /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349     G-[AGNSVY]-G-G-[AISV]  
BINDING     153     158       /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349     N-R-T-x(2)-[KR]  
BINDING     19     21       /ligand="shikimate" /ligand_id="ChEBI:CHEBI:36208     [ST]-x-[ST]  
ACT_SITE     70     70       Proton acceptor     K  
BINDING     82     82       /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349     [DE]  
BINDING     66     66       /ligand="shikimate" /ligand_id="ChEBI:CHEBI:36208     [TS]  
BINDING     91     91       /ligand="shikimate" /ligand_id="ChEBI:CHEBI:36208     N  
BINDING     106     106       /ligand="shikimate" /ligand_id="ChEBI:CHEBI:36208     D  
BINDING     214     214       /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349     [IVMLCATFG]  
BINDING     216     216       /ligand="shikimate" /ligand_id="ChEBI:CHEBI:36208     Y  
BINDING     235     235       /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349     G  
BINDING     242     242       /ligand="shikimate" /ligand_id="ChEBI:CHEBI:36208     Q  

Additional information [?]

Size range 253-295 amino acids
Related rules MF_01578 (YDIB supersedes the current rule); MF_03143 (ARO1 supersedes the current rule)
Fusion Nter: MF_00214 (aroD); Cter: None
Comments Aroe in CORGL and CORGB catalyzes the NAD(+)-dependent oxidation of both quinate and shikimate with a higher catalytic efficiency for quinate rather than shikimate, and seems to play a key role in the quinate-degradation pathway (PubMed=18566515).