HAMAP rule MF_00222

General rule information [?]

Accession	MF_00222
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 41)

Name	Shikimate_DH_AroE

Scope

Bacteria

Archaea

Templates

P15770 (AROE_ECOLI); O67049 (AROE_AQUAE); P43876 (AROE_HAEIN); P56119 (AROE_HELPY); Q8Y9N5 (AROE_LISMO); Q5HNV1 (AROE_STAEQ); Q5SJF8 (AROE_THET8); Q9KVT3 (AROE_VIBCH); Q58484 (AROE_METJA); P44774 (SHDHL_HAEIN); Q9X5C9 (AROE_CORGL): [Recover all]

Triggered by

HAMAP; MF_00222 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

AROE

Protein name

RecName:		Full=Shikimate dehydrogenase (NADP(+));
		Short=SDH;
		EC 1.1.1.25;

Gene name

aroE

Comments [?]

Function	Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).
Catalytic activity	RHEA:17737: NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH EC 1.1.1.25
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.
Subunit	Homodimer.
Similarity	Belongs to the shikimate dehydrogenase family.

Keywords [?]

Amino-acid biosynthesis
Aromatic amino acid biosynthesis
NADP
Oxidoreductase

Gene Ontology [?]

GO:0004764; Molecular function: shikimate 3-dehydrogenase (NADP+) activity.
GO:0009073; Biological process: aromatic amino acid family biosynthetic process.

Cross-references [?]

Pfam	PF01488; Shikimate_DH; 1;
	PF08501; Shikimate_dh_N; 1;
NCBIfam	TIGR00507; AroE; 1;

Features [?]

From: AROE_AQUAE (O67049)

Key From To Description Tag Condition FTGroup

BINDING 130 134 /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349 G-[AGNSVY]-G-G-[AISV]

BINDING 153 158 /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349 N-R-T-x(2)-[KR]

BINDING 19 21 /ligand="shikimate" /ligand_id="ChEBI:CHEBI:36208 [ST]-x-[ST]

ACT_SITE 70 70 Proton acceptor K

BINDING 82 82 /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349 [DE]

BINDING 66 66 /ligand="shikimate" /ligand_id="ChEBI:CHEBI:36208 [TS]

BINDING 91 91 /ligand="shikimate" /ligand_id="ChEBI:CHEBI:36208 N

BINDING 106 106 /ligand="shikimate" /ligand_id="ChEBI:CHEBI:36208 D

BINDING 214 214 /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349 [IVMLCATFG]

BINDING 216 216 /ligand="shikimate" /ligand_id="ChEBI:CHEBI:36208 Y

BINDING 235 235 /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349 G

BINDING 242 242 /ligand="shikimate" /ligand_id="ChEBI:CHEBI:36208 Q

Additional information [?]

Size range	253-295 amino acids
Related rules	MF_01578 (YDIB supersedes the current rule); MF_03143 (ARO1 supersedes the current rule)
Fusion	Nter: MF_00214 (aroD); Cter: None
Comments	Aroe in CORGL and CORGB catalyzes the NAD(+)-dependent oxidation of both quinate and shikimate with a higher catalytic efficiency for quinate rather than shikimate, and seems to play a key role in the quinate-degradation pathway (PubMed=18566515).

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