HAMAP rule MF_00269

General rule information [?]

Accession	MF_00269
Dates	1-JUN-2001 (Created) 3-APR-2020 (Last updated, Version 22)
Name	Tpx
Scope(s)	Bacteria
Template(s)	P0A862 (TPX_ECOLI); [ Recover all ]
Triggered by	HAMAP; MF_00269 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	TPX
Protein name	RecName: Full=Thiol peroxidase; Short=Tpx; EC=1.11.1.24; AltName: Full=Peroxiredoxin tpx; Short=Prx; AltName: Full=Thioredoxin peroxidase; AltName: Full=Thioredoxin-dependent peroxiredoxin;
Gene name	Name=tpx;

Comments [?]

FUNCTION	Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.
CATALYTIC ACTIVITY	Reaction=[thioredoxin]-dithiol + a hydroperoxide = [thioredoxin]- disulfide + an alcohol + H2O; Xref=Rhea:RHEA:62620, Rhea:RHEA- COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:15377, ChEBI:CHEBI:29950, ChEBI:CHEBI:30879, ChEBI:CHEBI:35924, ChEBI:CHEBI:50058; EC=1.11.1.24;
SUBUNIT	Homodimer.
case <FTTag:disulf>
MISCELLANEOUS	The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this atypical 2-Cys peroxiredoxin, C(R) is present in the same subunit to form an intramolecular disulfide. The disulfide is subsequently reduced by thioredoxin.
end case
SIMILARITY	Belongs to the peroxiredoxin family. Tpx subfamily.

Keywords [?]

Gene Ontology [?]

GO:0016209; Molecular function:antioxidant activity

GO:0008379; Molecular function:thioredoxin peroxidase activity

Cross-references [?]

Features [?]

From: TPX_ECOLI (P0A862)

Key

From

Description

Tag

Condition

FTGroup

DOMAIN

168

/note="Thioredoxin"

ACT_SITE

/note="Cysteine sulfenic acid (-SOH) intermediate"

DISULFID

/note="Redox-active"

disulf

C-x*-C

Additional information [?]