HAMAP rule MF_00269
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00269 |
| Accession | MF_00269 |
| Dates | 28-FEB-2005 (Created)
02-SEP-2024 (Last updated, Version 17) |
| Name | Tpx |
| Scope(s) |
Bacteria |
| Template(s) | P0A862; [ Recover all ] |
| Triggered by |
HAMAP; MF_00269 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | TPX |
| Protein name | RecName: Full=Thiol peroxidase; Short=Tpx; EC=1.11.1.24; AltName: Full=Peroxiredoxin tpx; Short=Prx; AltName: Full=Thioredoxin peroxidase; AltName: Full=Thioredoxin-dependent peroxiredoxin; |
| Gene name | Name=tpx; |
Comments
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| FUNCTION | Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. |
| CATALYTIC ACTIVITY | Reaction=a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O; Xref=Rhea:RHEA:62620, Rhea:RHEA- COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:15377, ChEBI:CHEBI:29950, ChEBI:CHEBI:30879, ChEBI:CHEBI:35924, ChEBI:CHEBI:50058; EC=1.11.1.24; |
| SUBUNIT | Homodimer. |
| case <FTTag:disulf> | |
| MISCELLANEOUS | The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this atypical 2-Cys peroxiredoxin, C(R) is present in the same subunit to form an intramolecular disulfide. The disulfide is subsequently reduced by thioredoxin. |
| end case | |
| SIMILARITY | Belongs to the peroxiredoxin family. Tpx subfamily. |
Keywords
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| Antioxidant | |
| Oxidoreductase | |
| Peroxidase | |
| Redox-active center | |
| case <FTTag:disulf> | |
| Disulfide bond | |
| end case | |
Gene Ontology
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| GO:0016209; Molecular function:antioxidant activity |
| GO:0008379; Molecular function:thioredoxin peroxidase activity |
Cross-references
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Features
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| From: TPX_ECOLI (P0A862) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DOMAIN | 19 | 168 | /note="Thioredoxin" | |||||||||
| ACT_SITE | 61 | 61 | /note="Cysteine sulfenic acid (-SOH) intermediate" | C | ||||||||
| DISULFID | 61 | 95 | /note="Redox-active" | disulf | C-x*-C | |||||||
Additional information
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| Size range | 161-175 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |