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Annotation rule MF_00269
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General rule information [?]

Accession MF_00269
Dates 1-JUN-2001 (Created)
3-APR-2020 (Last updated, Version 22)
Name Tpx
Scope
Bacteria
Template P0A862 (TPX_ECOLI)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
TPX
Protein name
RecName: Full=Thiol peroxidase;
Short=Tpx;
EC 1.11.1.24;
AltName: Full=Peroxiredoxin tpx;
Short=Prx;
AltName: Full=Thioredoxin peroxidase;
AltName: Full=Thioredoxin-dependent peroxiredoxin;
Gene name
tpx

Comments [?]

Function Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.
Catalytic activity RHEA:62620: [thioredoxin]-dithiol + a hydroperoxide = [thioredoxin]-disulfide + an alcohol + H2O
EC 1.11.1.24
Subunit Homodimer.
case <FTTag:disulf>
Miscellaneous The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this atypical 2-Cys peroxiredoxin, C(R) is present in the same subunit to form an intramolecular disulfide. The disulfide is subsequently reduced by thioredoxin.
end case
Similarity Belongs to the peroxiredoxin family. Tpx subfamily.

Keywords [?]

Antioxidant
Oxidoreductase
Peroxidase
Redox-active center
case <FTTag:disulf>
Disulfide bond
end case

Gene Ontology [?]

GO:0016209; Molecular function: antioxidant activity.
GO:0008379; Molecular function: thioredoxin peroxidase activity.

Cross-references [?]

Pfam PF08534; Redoxin; 1;
PROSITE PS01265; TPX; 1;
PS51352; THIOREDOXIN_2; 1;

Features [?]

From: TPX_ECOLI (P0A862)
Key     From     To       Description   Tag   Condition   FTGroup
DOMAIN     19     168       Thioredoxin        
ACT_SITE     61     61       Cysteine sulfenic acid (-SOH) intermediate     C  
DISULFID     61     95       Redox-active   disulf   C-x*-C  

Additional information [?]

Size range 161-175 amino acids
Related rules None
Fusion None


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