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HAMAP rule MF_00269

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General rule information [?]

Accession MF_00269
Dates 1-JUN-2001 (Created)
3-APR-2020 (Last updated, Version 22)
Name Tpx
Scope(s) Bacteria
Template(s) P0A862 (TPX_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00269 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier TPX
Protein name RecName: Full=Thiol peroxidase;
AltName: Full=Peroxiredoxin tpx;
AltName: Full=Thioredoxin peroxidase;
AltName: Full=Thioredoxin-dependent peroxiredoxin;
Gene name Name=tpx;

Comments [?]

FUNCTIONThiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.
CATALYTIC ACTIVITY Reaction=[thioredoxin]-dithiol + a hydroperoxide = [thioredoxin]- disulfide + an alcohol + H2O; Xref=Rhea:RHEA:62620, Rhea:RHEA- COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:15377, ChEBI:CHEBI:29950, ChEBI:CHEBI:30879, ChEBI:CHEBI:35924, ChEBI:CHEBI:50058; EC=;
case <FTTag:disulf>
MISCELLANEOUSThe active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this atypical 2-Cys peroxiredoxin, C(R) is present in the same subunit to form an intramolecular disulfide. The disulfide is subsequently reduced by thioredoxin.
end case
SIMILARITYBelongs to the peroxiredoxin family. Tpx subfamily.

Keywords [?]

Gene Ontology [?]

GO:0016209; Molecular function:antioxidant activity
GO:0008379; Molecular function:thioredoxin peroxidase activity

Cross-references [?]

Pfam PF08534; Redoxin; 1;
PROSITE PS01265; TPX; 1;

Features [?]

From: TPX_ECOLI (P0A862)
Key From To Description Tag Condition FTGroup
DOMAIN 19 168 /note="Thioredoxin"
ACT_SITE 61 61 /note="Cysteine sulfenic acid (-SOH) intermediate" C
DISULFID 61 95 /note="Redox-active" disulf C-x*-C

Additional information [?]

Size range 161-175 amino acids
Related rules None
Fusion Nter: None Cter: None

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