HAMAP rule MF_00277
General rule information
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Accession | MF_00277 |
Dates | 1-JUN-2001 (Created) 18-NOV-2019 (Last updated, Version 30) |
Name | PII_uridylyl_transf |
Scope | Bacteria |
Templates | P27249 (GLND_ECOLI); Q8RQD1 (GLND_AZOBR); P36223 (GLND_AZOVI); P9WN29 (GLND_MYCTU); P41393 (GLND_KLEOX); Q9RAE4 (GLND_RHILV): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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case defined <Property:NITROGEN_FIXATION> and <Property:NITROGEN_FIXATION>
Function | Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism. |
else case defined <Property:NITROGEN_FIXATION> and not <Property:NITROGEN_FIXATION>
Function | Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism. |
else
Function | Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen metabolism. |
end case
Catalytic activity | RHEA:13673: [protein-PII]-L-tyrosine + UTP = [protein-PII]-uridylyl-L-tyrosine + diphosphate
EC 2.7.7.59 |
RHEA:48600: [protein-PII]-uridylyl-L-tyrosine + H2O = [protein-PII]-L-tyrosine + H(+) + UMP | |
Cofactor | Mg(2+) |
Activity regulation | Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity. |
Domain | Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing. |
Similarity | Belongs to the GlnD family. |
Keywords
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case <Property:NITROGEN_FIXATION>
end case
Gene Ontology
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GO:0008773; Molecular function: [protein-PII] uridylyltransferase activity.
GO:0008081; Molecular function: phosphoric diester hydrolase activity.
GO:0006808; Biological process: regulation of nitrogen utilization.
GO:0008081; Molecular function: phosphoric diester hydrolase activity.
GO:0006808; Biological process: regulation of nitrogen utilization.
case <Property:NITROGEN_FIXATION>
GO:0009399; Biological process: nitrogen fixation.
end case
Cross-references
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PROSITE | PS51671; ACT; 1; trigger=PRU01007; |
Pfam | PF01842; ACT; 1; |
PF01966; HD; 1; | |
PF01909; NTP_transf_2; 1; | |
PIRSF | PIRSF006288; PII_uridyltransf; 1; |
TIGRFAMs | TIGR01693; UTase_glnD; 1; |
PROSITE | PS51831; HD; 1; trigger=PRU01175; |
Features
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From: GLND_ECOLI (P27249) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | Nter | 349 | Uridylyltransferase | |||||||||
REGION | 350 | 708 | Uridylyl-removing |
Additional information
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Size range | 808-949 amino acids |
Related rules | None |
Fusion | None |
Comments | Divergent CORGL not shown in alignment and not used in size range |