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HAMAP rule MF_00277

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General rule information [?]

Accession MF_00277
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 31)
Name PII_uridylyl_transf
Scope(s) Bacteria
Template(s) P27249 (GLND_ECOLI); Q8RQD1 (GLND_AZOBR); P36223 (GLND_AZOVI); P9WN29 (GLND_MYCTU); P41393 (GLND_KLEOX); Q9RAE4 (GLND_RHILV); [ Recover all ]
Triggered by HAMAP; MF_00277 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier GLND
Protein name RecName: Full=Bifunctional uridylyltransferase/uridylyl-removing enzyme;
                 Short=UTase/UR;
AltName: Full=Bifunctional nitrogen sensor protein;
AltName: Full=Bifunctional [protein-PII] modification enzyme;
                 Includes:
RecName: Full=[Protein-PII] uridylyltransferase;
                 Short=PII uridylyltransferase;
                 Short=UTase;
                 EC=2.7.7.59;
                 Includes:
RecName: Full=[Protein-PII]-UMP uridylyl-removing enzyme;
                 Short=UR;
                 EC=3.1.4.-;
Gene name Name=glnD;

Comments [?]

case defined <Property:NITROGEN_FIXATION> and <Property:NITROGEN_FIXATION>
FUNCTIONModifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism.
else case defined <Property:NITROGEN_FIXATION> and not <Property:NITROGEN_FIXATION>
FUNCTIONModifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.
else
FUNCTIONModifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen metabolism.
end case
CATALYTIC ACTIVITY Reaction=[protein-PII]-L-tyrosine + UTP = [protein-PII]-uridylyl-L- tyrosine + diphosphate; Xref=Rhea:RHEA:13673, Rhea:RHEA-COMP:12147, Rhea:RHEA-COMP:12148, ChEBI:CHEBI:33019, ChEBI:CHEBI:46398, ChEBI:CHEBI:46858, ChEBI:CHEBI:90602; EC=2.7.7.59;
CATALYTIC ACTIVITY Reaction=[protein-PII]-uridylyl-L-tyrosine + H2O = [protein-PII]-L- tyrosine + H(+) + UMP; Xref=Rhea:RHEA:48600, Rhea:RHEA-COMP:12147, Rhea:RHEA-COMP:12148, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:46858, ChEBI:CHEBI:57865, ChEBI:CHEBI:90602;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
ACTIVITY REGULATIONUridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.
DOMAINHas four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.
SIMILARITYBelongs to the GlnD family.

Keywords [?]


Gene Ontology [?]

GO:0008773; Molecular function:[protein-PII] uridylyltransferase activity
GO:0008081; Molecular function:phosphoric diester hydrolase activity
GO:0006808; Biological process:regulation of nitrogen utilization
case <Property:NITROGEN_FIXATION>
GO:0009399; Biological process:nitrogen fixation
end case

Cross-references [?]

PROSITE PS51671; ACT; 1;
Pfam PF01842; ACT; 1;
Pfam PF01966; HD; 1;
Pfam PF01909; NTP_transf_2; 1;
PIRSF PIRSF006288; PII_uridyltransf; 1;
NCBIfam TIGR01693; UTase_glnD; 1;
PROSITE PS51831; HD; 1;

Features [?]

From: GLND_ECOLI (P27249)
Key From To Description Tag Condition FTGroup
REGION Nter 349 /note="Uridylyltransferase"
REGION 350 708 /note="Uridylyl-removing"

Additional information [?]

Size range 808-949 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Divergent CORGL not shown in alignment and not used in size range



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