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Annotation rule MF_00279
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General rule information [?]

Accession MF_00279
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 27)
Name PdxJ
Scope
Bacteria
Template P0A794 (PDXJ_ECOLI)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PDXJ
Protein name
RecName: Full=Pyridoxine 5'-phosphate synthase;
Short=PNP synthase;
EC 2.6.99.2;
Gene name
pdxJ

Comments [?]

Function Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate.
Catalytic activity RHEA:15265: 1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = H(+) + 2 H2O + phosphate + pyridoxine 5'-phosphate
EC 2.6.99.2
Pathway Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 5/5.
Subunit Homooctamer; tetramer of dimers.
Subcellular location Cytoplasm.
Similarity Belongs to the PNP synthase family.

Keywords [?]


Gene Ontology [?]

GO:0016769; Molecular function: transferase activity, transferring nitrogenous groups.
GO:0008615; Biological process: pyridoxine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF03740; PdxJ; 1;
TIGRFAMs TIGR00559; PdxJ; 1;

Features [?]

From: PDXJ_ECOLI (P0A794)
Key     From     To       Description   Tag   Condition   FTGroup
REGION (Optional)     11     12       1-deoxy-D-xylulose 5-phosphate binding     D-H  
REGION     215     216       3-amino-2-oxopropyl phosphate binding     G-[HQFY]  
ACT_SITE     45     45       Proton acceptor     H  
ACT_SITE     72     72       Proton acceptor     E  
ACT_SITE     193     193       Proton donor     H  
BINDING     9     9       3-amino-2-oxopropyl phosphate     N  
BINDING     20     20       3-amino-2-oxopropyl phosphate     R  
BINDING     47     47       1-deoxy-D-xylulose 5-phosphate     R  
BINDING     52     52       1-deoxy-D-xylulose 5-phosphate     H  
BINDING     102     102       1-deoxy-D-xylulose 5-phosphate     T  
BINDING (Optional)     194     194       3-amino-2-oxopropyl phosphate; via amide nitrogen     [GASD]  
SITE     153     153       Transition state stabilizer     E  

Additional information [?]

Size range 232-267 amino acids
Related rules None
Fusion None