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HAMAP rule MF_00279

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General rule information [?]

Accession MF_00279
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 31)
Name PdxJ
Scope(s) Bacteria
Template(s) P0A794 (PDXJ_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00279 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PDXJ
Protein name RecName: Full=Pyridoxine 5'-phosphate synthase;
                 Short=PNP synthase;
Gene name Name=pdxJ;

Comments [?]

FUNCTIONCatalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino- 2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate.
CATALYTIC ACTIVITY Reaction=1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = H(+) + 2 H2O + phosphate + pyridoxine 5'-phosphate; Xref=Rhea:RHEA:15265, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:43474, ChEBI:CHEBI:57279, ChEBI:CHEBI:57792, ChEBI:CHEBI:58589; EC=;
PATHWAYCofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 5/5.
SUBUNITHomooctamer; tetramer of dimers.
SIMILARITYBelongs to the PNP synthase family.

Keywords [?]

Gene Ontology [?]

GO:0016769; Molecular function:transferase activity, transferring nitrogenous groups
GO:0008615; Biological process:pyridoxine biosynthetic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF03740; PdxJ; 1;
NCBIfam TIGR00559; PdxJ; 1;

Features [?]

From: PDXJ_ECOLI (P0A794)
Key From To Description Tag Condition FTGroup
BINDING 11 12 /ligand="1-deoxy-D-xylulose 5-phosphate"
BINDING 215 216 /ligand="3-amino-2-oxopropyl phosphate"
ACT_SITE 45 45 /note="Proton acceptor" H
ACT_SITE 72 72 /note="Proton acceptor" E
ACT_SITE 193 193 /note="Proton donor" H
BINDING 9 9 /ligand="3-amino-2-oxopropyl phosphate"
BINDING 20 20 /ligand="3-amino-2-oxopropyl phosphate"
BINDING 47 47 /ligand="1-deoxy-D-xylulose 5-phosphate"
BINDING 52 52 /ligand="1-deoxy-D-xylulose 5-phosphate"
BINDING 102 102 /ligand="1-deoxy-D-xylulose 5-phosphate"
BINDING 194 194 /ligand="3-amino-2-oxopropyl phosphate"
SITE 153 153 /note="Transition state stabilizer" E

Additional information [?]

Size range 232-267 amino acids
Related rules None
Fusion Nter: None Cter: None

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