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Annotation rule MF_00297
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General rule information [?]

Accession MF_00297
Dates 1-JUN-2001 (Created)
9-SEP-2020 (Last updated, Version 26)
Name Nudix_NudC
Template P32664 (NUDC_ECOLI)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=NAD-capped RNA hydrolase NudC;
Short=DeNADding enzyme NudC;
EC 3.6.1.-;
AltName: Full=NADH pyrophosphatase;
Gene name

Comments [?]

Function mRNA decapping enzyme that specifically removes the nicotinamide adenine dinucleotide (NAD) cap from a subset of mRNAs by hydrolyzing the diphosphate linkage to produce nicotinamide mononucleotide (NMN) and 5' monophosphate mRNA. The NAD-cap is present at the 5'-end of some mRNAs and stabilizes RNA against 5'-processing. Has preference for mRNAs with a 5'-end purine. Catalyzes the hydrolysis of a broad range of dinucleotide pyrophosphates.
Catalytic activity RHEA:60876: a 5'-end NAD(+)-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-adenosine-phospho-ribonucleoside in mRNA + beta-nicotinamide D-ribonucleotide + 2 H(+)
PhysiologicalDirection=left-to-right (RHEA:60877)
RHEA:11800: H2O + NAD(+) = AMP + beta-nicotinamide D-ribonucleotide + 2 H(+)
RHEA:48868: H2O + NADH = AMP + 2 H(+) + reduced beta-nicotinamide D-ribonucleotide
Cofactor Mg(2+)
Note: Divalent metal cations. Mg(2+) or Mn(2+).
case <FTTag:zinc>
Cofactor Zn(2+)
Note: Binds 1 zinc ion per subunit.
end case
Subunit Homodimer.
Similarity Belongs to the Nudix hydrolase family. NudC subfamily.

Keywords [?]

case <FTTag:zinc>
end case

Gene Ontology [?]

GO:0000210; Molecular function: NAD+ diphosphatase activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0030145; Molecular function: manganese ion binding.
case <FTTag:zinc>
GO:0008270; Molecular function: zinc ion binding.
end case

Cross-references [?]

Pfam PF00293; NUDIX; 1;
PF09296; NUDIX-like; 1;
PF09297; zf-NADH-PPase; 1;
PROSITE PS51462; NUDIX; 1; trigger=PRU00794;
PS00893; NUDIX_BOX; 1;

Features [?]

From: NUDC_ECOLI (P32664)
Key     From     To       Description   Tag   Condition   FTGroup
REGION (Optional)     192     199       Substrate binding     Q-x-W-[PA]-F-P-x-[SN]  
MOTIF     159     180       Nudix box        
METAL (Optional)     98     98       Zinc   zinc   C  
METAL (Optional)     101     101       Zinc   zinc   C  
METAL (Optional)     116     116       Zinc   zinc   C  
METAL (Optional)     119     119       Zinc   zinc   C  
METAL     158     158       Divalent metal cation 1; via carbonyl oxygen     A  
METAL     174     174       Divalent metal cation 2     E  
METAL     174     174       Divalent metal cation 3     E  
METAL     178     178       Divalent metal cation 1     E  
METAL     178     178       Divalent metal cation 3     E  
METAL     219     219       Divalent metal cation 1     E  
METAL     219     219       Divalent metal cation 3     E  
BINDING (Optional)     25     25       Substrate     K  
BINDING (Optional)     69     69       Substrate     R  
BINDING (Optional)     111     111       Substrate     E  
BINDING (Optional)     124     124       Substrate     Y  
BINDING (Optional)     241     241       Substrate; via amide nitrogen     A  

Additional information [?]

Size range 257-313 amino acids
Related rules None
Fusion None
Comments Possible wrong start in MYCTU