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HAMAP rule MF_00304

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General rule information [?]

Accession MF_00304
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 31)
Name Thi4
Scope
Bacteria
Archaea
Templates Q58018 (THI4_METJA); P32318 (THI4_YEAST); D4GSS5 (THI4_HALVD): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
THI4
case <FTTag:dehydroala>
Protein name
RecName: Full=Thiamine thiazole synthase;
EC 2.4.2.60;
else
Protein name
RecName: Full=Thiamine thiazole synthase;
EC 2.4.2.59;
end case
Gene name
thi4

Comments [?]

case <FTTag:dehydroala>
Function Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme.
Catalytic activity RHEA:55708: [ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 2 H(+) + 3 H2O + nicotinamide
EC 2.4.2.60
Ptm During the catalytic reaction, a sulfide is transferred from #{Cys-165} to a reaction intermediate, generating a dehydroalanine residue.
else
Function Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.
Catalytic activity RHEA:55704: glycine + hydrogen sulfide + NAD(+) = ADP-5-ethyl-4-methylthiazole-2-carboxylate + H(+) + 3 H2O + nicotinamide
EC 2.4.2.59
end case
Cofactor Fe(2+)
Pathway Cofactor biosynthesis; thiamine diphosphate biosynthesis.
Subunit Homooctamer; tetramer of dimers.
Similarity Belongs to the THI4 family.

Keywords [?]

Iron
Metal-binding
NAD
Thiamine biosynthesis
Transferase

Gene Ontology [?]

GO:0005506; Molecular function: iron ion binding.
GO:0016763; Molecular function: pentosyltransferase activity.
GO:0009228; Biological process: thiamine biosynthetic process.
GO:0052837; Biological process: thiazole biosynthetic process.

Cross-references [?]

NCBIfam TIGR00292; TIGR00292; 1;

Features [?]

From: THI4_METJA (Q58018)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     66     67       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between two adjacent protomers" /note="in other chain     E-[RKQE]  
BINDING (Optional)     164     166       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between two adjacent protomers     H-x-D  
case not <FT:2>
BINDING     166     166       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between two adjacent protomers     D  
end case
BINDING     166     166       /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_note="ligand shared between two adjacent protomers     D  
BINDING     181     181       /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_note="ligand shared between two adjacent protomers" /note="in other chain     H  
BINDING (Optional)     47     47       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between two adjacent protomers" /note="in other chain     S  
case not <FT:6>
BINDING     47     47       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between two adjacent protomers" /note="in other chain     A  
end case
BINDING     74     74       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between two adjacent protomers" /note="in other chain     G  
BINDING (Optional)     138     138       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between two adjacent protomers" /note="in other chain     [VIL]  
BINDING (Optional)     184     184       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between two adjacent protomers" /note="in other chain     S  
BINDING     230     230       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between two adjacent protomers" /note="in other chain     [MLI]  
BINDING     240     240       /ligand="glycine" /ligand_id="ChEBI:CHEBI:57305     R  
From: THI4_HALVD (D4GSS5)
MOD_RES (Optional)     165     165       2,3-didehydroalanine (Cys)   dehydroala   C  

Additional information [?]

Size range 250-310 amino acids
Related rules None
Fusion None
Comments None.


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