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HAMAP rule MF_00304

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General rule information [?]

Accession MF_00304
Dates 1-JUN-2001 (Created)
1-MAR-2024 (Last updated, Version 32)
Name Thi4
Scope(s) Bacteria
Archaea
Template(s) Q58018 (THI4_METJA); P32318 (THI4_YEAST); D4GSS5 (THI4_HALVD); [ Recover all ]
Triggered by HAMAP; MF_00304 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier THI4
case <FTTag:dehydroala>
Protein name RecName: Full=Thiamine thiazole synthase;
                 EC=2.4.2.60;
else
Protein name RecName: Full=Thiamine thiazole synthase;
                 EC=2.4.2.59;
end case
Gene name Name=thi4;

Comments [?]

case <FTTag:dehydroala>
FUNCTIONInvolved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5- (2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron- dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme.
CATALYTIC ACTIVITY Reaction=[ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP- thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2- carboxylate + 2 H(+) + 3 H2O + nicotinamide; Xref=Rhea:RHEA:55708, Rhea:RHEA-COMP:14264, Rhea:RHEA-COMP:14265, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17154, ChEBI:CHEBI:29950, ChEBI:CHEBI:57305, ChEBI:CHEBI:57540, ChEBI:CHEBI:90873, ChEBI:CHEBI:139151; EC=2.4.2.60;
PTMDuring the catalytic reaction, a sulfide is transferred from #{Cys-165} to a reaction intermediate, generating a dehydroalanine residue.
else
FUNCTIONInvolved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.
CATALYTIC ACTIVITY Reaction=glycine + hydrogen sulfide + NAD(+) = ADP-5-ethyl-4- methylthiazole-2-carboxylate + H(+) + 3 H2O + nicotinamide; Xref=Rhea:RHEA:55704, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17154, ChEBI:CHEBI:29919, ChEBI:CHEBI:57305, ChEBI:CHEBI:57540, ChEBI:CHEBI:139151; EC=2.4.2.59;
end case
COFACTOR Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
PATHWAYCofactor biosynthesis; thiamine diphosphate biosynthesis.
SUBUNITHomooctamer; tetramer of dimers.
SIMILARITYBelongs to the THI4 family.

Keywords [?]

Iron
Metal-binding
NAD
Thiamine biosynthesis
Transferase

Gene Ontology [?]

GO:0005506; Molecular function:iron ion binding
GO:0016763; Molecular function:pentosyltransferase activity
GO:0009229; Biological process:thiamine diphosphate biosynthetic process
GO:0052837; Biological process:thiazole biosynthetic process

Cross-references [?]

NCBIfam TIGR00292; TIGR00292; 1;

Features [?]

From: THI4_METJA (Q58018)
Key From To Description Tag Condition FTGroup
BINDING 66 67 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
/ligand_note="ligand shared between two adjacent protomers"
/note="in other chain"		 E-[RKQE]
BINDING 164 166 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
/ligand_note="ligand shared between two adjacent protomers"		 H-x-D
case not <FT:2>
BINDING 166 166 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
/ligand_note="ligand shared between two adjacent protomers"		 D
end case
BINDING 166 166 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"
/ligand_note="ligand shared between two adjacent protomers"		 D
BINDING 181 181 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"
/ligand_note="ligand shared between two adjacent protomers"
/note="in other chain"		 H
BINDING 47 47 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
/ligand_note="ligand shared between two adjacent protomers"
/note="in other chain"		 S
case not <FT:6>
BINDING 47 47 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
/ligand_note="ligand shared between two adjacent protomers"
/note="in other chain"		 A
end case
BINDING 74 74 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
/ligand_note="ligand shared between two adjacent protomers"
/note="in other chain"		 G
BINDING 138 138 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
/ligand_note="ligand shared between two adjacent protomers"
/note="in other chain"		 [VIL]
BINDING 184 184 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
/ligand_note="ligand shared between two adjacent protomers"
/note="in other chain"		 S
BINDING 230 230 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
/ligand_note="ligand shared between two adjacent protomers"
/note="in other chain"		 [MLI]
BINDING 240 240 /ligand="glycine"
/ligand_id="ChEBI:CHEBI:57305"		 R
From: THI4_HALVD (D4GSS5)
Key From To Description Tag Condition FTGroup
MOD_RES 165 165 /note="2,3-didehydroalanine (Cys)" dehydroala C

Additional information [?]

Size range 250-310 amino acids
Related rules None
Fusion Nter: None Cter: None



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