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Annotation rule MF_00304
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General rule information [?]

Accession MF_00304
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 23)
Name Thi4
Scope
Bacteria
Archaea
Templates Q58018 (THI4_METJA); P32318 (THI4_YEAST): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
THI4
Protein name
RecName: Full=Thiamine thiazole synthase;
EC 2.4.2.59;
Gene name
thi4

Comments [?]

Function Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.
Catalytic activity RHEA:55704: glycine + hydrogen sulfide + NAD(+) = ADP-5-ethyl-4-methylthiazole-2-carboxylate + H(+) + 3 H2O + nicotinamide
EC 2.4.2.59
Cofactor Fe(2+)
Pathway Cofactor biosynthesis; thiamine diphosphate biosynthesis.
Subunit Homooctamer; tetramer of dimers.
Similarity Belongs to the THI4 family.

Keywords [?]

Iron
Metal-binding
NAD
Thiamine biosynthesis
Transferase

Gene Ontology [?]

GO:0005506; Molecular function: iron ion binding.
GO:0016763; Molecular function: transferase activity, transferring pentosyl groups.
GO:0009228; Biological process: thiamine biosynthetic process.
GO:0052837; Biological process: thiazole biosynthetic process.

Cross-references [?]

TIGRFAMs TIGR00292; TIGR00292; 1;

Features [?]

From: THI4_METJA (Q58018)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     66     67       NAD     E-[RKQE]  
NP_BIND (Optional)     164     166       NAD; shared with adjacent protomer     H-x-D  
case not <FT:2>
BINDING     166     166       NAD; shared with adjacent protomer     D  
end case
METAL     166     166       Iron; shared with adjacent protomer     D  
METAL     181     181       Iron     H  
BINDING (Optional)     47     47       NAD     S  
case not <FT:6>
BINDING     47     47       NAD; via amide nitrogen     A  
end case
BINDING     74     74       NAD; via amide nitrogen and carbonyl oxygen     G  
BINDING (Optional)     138     138       NAD; via amide nitrogen and carbonyl oxygen     [VIL]  
BINDING (Optional)     184     184       NAD; via carbonyl oxygen     S  
BINDING     230     230       NAD; via amide nitrogen     [MLI]  
BINDING     240     240       Glycine     R  

Additional information [?]

Size range 250-310 amino acids
Related rules None
Fusion None
Comments None.


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