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Annotation rule MF_00304 |
Accession | MF_00304 |
Dates | 1-JUN-2001 (Created) 18-NOV-2019 (Last updated, Version 23) |
Name | Thi4 |
Scope | Bacteria
Archaea |
Templates | Q58018 (THI4_METJA); P32318 (THI4_YEAST): [Recover all] |
Triggered by |
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Protein name |
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Gene name |
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Function | Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur. |
Catalytic activity | RHEA:55704: glycine + hydrogen sulfide + NAD(+) = ADP-5-ethyl-4-methylthiazole-2-carboxylate + H(+) + 3 H2O + nicotinamide
EC 2.4.2.59 |
Cofactor | Fe(2+) |
Pathway | Cofactor biosynthesis; thiamine diphosphate biosynthesis. |
Subunit | Homooctamer; tetramer of dimers. |
Similarity | Belongs to the THI4 family. |
TIGRFAMs | TIGR00292; TIGR00292; 1; |
From: THI4_METJA (Q58018) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
NP_BIND | 66 | 67 | NAD | E-[RKQE] | ||||||||
NP_BIND (Optional) | 164 | 166 | NAD; shared with adjacent protomer | H-x-D |
METAL | 166 | 166 | Iron; shared with adjacent protomer | D | ||||||||
METAL | 181 | 181 | Iron | H | ||||||||
BINDING (Optional) | 47 | 47 | NAD | S |
Size range | 250-310 amino acids |
Related rules | None |
Fusion | None |
Comments | None. |