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HAMAP rule MF_00318

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General rule information [?]

Accession MF_00318
Dates 1-JUN-2001 (Created)
30-JAN-2024 (Last updated, Version 64)
Name Enolase
Scope(s) Bacteria
Template(s) P0A6P9 (ENO_ECOLI); Q8GR70 (ENO_ENTHR); P37869 (ENO_BACSU); Q97QS2 (ENO_STRPN); P9WNL1 (ENO_MYCTU); [ Recover all ]
Triggered by HAMAP; MF_00318 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier ENO
Protein name RecName: Full=Enolase;
AltName: Full=2-phospho-D-glycerate hydro-lyase;
AltName: Full=2-phosphoglycerate dehydratase;
Gene name Name=eno;

Comments [?]

FUNCTIONCatalyzes the reversible conversion of 2-phosphoglycerate (2- PG) into phosphoenolpyruvate (PEP). It is essential for the degradation of carbohydrates via glycolysis.
CATALYTIC ACTIVITY Reaction=(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate; Xref=Rhea:RHEA:10164, ChEBI:CHEBI:15377, ChEBI:CHEBI:58289, ChEBI:CHEBI:58702; EC=;
case <FTGroup:1>
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds a second Mg(2+) ion via substrate during catalysis.;
end case
PATHWAYCarbohydrate degradation; glycolysis; pyruvate from D- glyceraldehyde 3-phosphate: step 4/5.
case <OC:Gammaproteobacteria>
SUBUNITComponent of the RNA degradosome, a multiprotein complex involved in RNA processing and mRNA degradation.
end case
SUBCELLULAR LOCATIONCytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. Once secreted, it may remain attached to the cell surface.
SIMILARITYBelongs to the enolase family.

Keywords [?]

Gene Ontology [?]

case <FTGroup:1>
GO:0000287; Molecular function:magnesium ion binding
end case
GO:0004634; Molecular function:phosphopyruvate hydratase activity
GO:0006096; Biological process:glycolytic process
GO:0005737; Cellular component:cytoplasm
GO:0009986; Cellular component:cell surface

Cross-references [?]

Pfam PF00113; Enolase_C; 1;
Pfam PF03952; Enolase_N; 1;
PIRSF PIRSF001400; Enolase; 1;
NCBIfam TIGR01060; eno; 1;

Features [?]

From: ENO_ECOLI (P0A6P9)
Key From To Description Tag Condition FTGroup
ACT_SITE 209 209 /note="Proton donor" [ED]
ACT_SITE 342 342 /note="Proton acceptor" K
BINDING 246 246 /ligand="Mg(2+)"
D 1
BINDING 290 290 /ligand="Mg(2+)"
E 1
BINDING 317 317 /ligand="Mg(2+)"
D 1
BINDING 167 167 /ligand="(2R)-2-phosphoglycerate"
BINDING 342 342 /ligand="(2R)-2-phosphoglycerate"
BINDING 371 371 /ligand="(2R)-2-phosphoglycerate"
BINDING 372 372 /ligand_id="ChEBI:CHEBI:58289"
BINDING 393 393 /ligand="(2R)-2-phosphoglycerate"

Additional information [?]

Size range 369-550 amino acids
Related rules None
Fusion Nter: None Cter: None

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