HAMAP rule MF_00333
General rule information
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| Accession | MF_00333 |
| Dates | 1-JUN-2001 (Created)
15-FEB-2024 (Last updated, Version 46) |
| Name | Coprogen_oxidas |
| Scope(s) |
Bacteria |
| Template(s) | P36553 (HEM6_ECOLI); P72848 (HEM6_SYNY3); [ Recover all ] |
| Triggered by |
HAMAP; MF_00333 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | HEM6 |
| Protein name | RecName: Full=Oxygen-dependent coproporphyrinogen-III oxidase; Short=CPO; Short=Coprogen oxidase; Short=Coproporphyrinogenase; EC=1.3.3.3; |
| Gene name | Name=hemF; |
Comments
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| case <OC:Cyanobacteriota> | |
| FUNCTION | Involved in the heme and chlorophyll biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX. |
| else | |
| FUNCTION | Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen- IX. |
| end case | |
| CATALYTIC ACTIVITY | Reaction=coproporphyrinogen III + 2 H(+) + O2 = 2 CO2 + 2 H2O + protoporphyrinogen IX; Xref=Rhea:RHEA:18257, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526, ChEBI:CHEBI:57307, ChEBI:CHEBI:57309; EC=1.3.3.3; |
| case <OC:Escherichia> | |
| COFACTOR | Name=Mn(2+); Xref=ChEBI:CHEBI:29035; |
| else | |
| COFACTOR | Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240; |
| end case | |
| PATHWAY | Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. |
| SUBUNIT | Homodimer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the aerobic coproporphyrinogen-III oxidase family. |
Keywords
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| case <OC:Cyanobacteriota> | |
| Chlorophyll biosynthesis | |
| end case | |
| Cytoplasm | |
| Heme biosynthesis | |
| case <OC:Escherichia> | |
| Manganese | |
| end case | |
| Metal-binding | |
| Oxidoreductase | |
| Porphyrin biosynthesis | |
Gene Ontology
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| GO:0004109; Molecular function:coproporphyrinogen oxidase activity | |
| case <OCellular component:Escherichia> | |
| GO:0030145; Molecular function:manganese ion binding | |
| end case | |
| GO:0042803; Molecular function:protein homodimerization activity | |
| GO:0006782; Biological process:protoporphyrinogen IX biosynthetic process | |
| GO:0006783; Biological process:heme biosynthetic process | |
| case <OCellular component:Cyanobacteriota> | |
| GO:0015995; Biological process:chlorophyll biosynthetic process | |
| end case | |
| GO:0005737; Cellular component:cytoplasm | |
Cross-references
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| Pfam | PF01218; Coprogen_oxidas; 1; |
| PRINTS | PR00073; COPRGNOXDASE; 1; |
| PROSITE | PS01021; COPROGEN_OXIDASE; 1; |
Features
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| From: HEM6_ECOLI (P36553) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 108 | 110 | /ligand="substrate" | N-x-R | ||||||||
| REGION | 240 | 275 | /note="Important for dimerization" | |||||||||
| BINDING | 258 | 260 | /ligand="substrate" | G-x-[NR] | ||||||||
| ACT_SITE | 106 | 106 | /note="Proton donor" | H | ||||||||
| case <OC:Escherichia> | ||||||||||||
| BINDING | 96 | 96 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" |
H | ||||||||
| BINDING | 106 | 106 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" |
H | ||||||||
| BINDING | 145 | 145 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" |
H | ||||||||
| BINDING | 175 | 175 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" |
H | ||||||||
| else | ||||||||||||
| BINDING | 96 | 96 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" |
H | ||||||||
| BINDING | 106 | 106 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" |
H | ||||||||
| BINDING | 145 | 145 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" |
H | ||||||||
| BINDING | 175 | 175 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" |
H | ||||||||
| end case | ||||||||||||
| BINDING | 92 | 92 | /ligand="substrate" | S | ||||||||
| SITE | 175 | 175 | /note="Important for dimerization" | H | ||||||||
Additional information
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| Size range | 279-363 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |