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HAMAP rule MF_00336

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General rule information [?]

Accession MF_00336
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 43)
Name BioD
Templates P13000 (BIOD1_ECOLI); P22818 (BIOD_LYSSH); P9WPQ5 (BIOD_MYCTU); O24872 (BIOD_HELPY): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=ATP-dependent dethiobiotin synthetase BioD;
AltName: Full=DTB synthetase;
AltName: Full=Dethiobiotin synthase;
Gene name

Comments [?]

Function Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.
Catalytic activity RHEA:15805: (7R,8S)-7,8-diammoniononanoate + ATP + CO2 = (4R,5S)-dethiobiotin + ADP + 3 H(+) + phosphate
Cofactor Mg(2+)
Pathway Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.
Subunit Homodimer.
Subcellular location Cytoplasm.
Similarity Belongs to the dethiobiotin synthetase family.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004141; Molecular function: dethiobiotin synthase activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0009102; Biological process: biotin biosynthetic process.

Cross-references [?]

NCBIfam TIGR00347; BioD; 1;
PIRSF PIRSF006755; DTB_synth; 1;

Features [?]

From: BIOD1_ECOLI (P13000)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     13     18       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     [EDNSG]-[VAILSC]-G-K-[TS]-[VLTIYFH]  
BINDING     116     119       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     E-x-x-G  
BINDING     176     177       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     [NSMVILG]-[DEHRQTNCSMK]  
BINDING     205     207       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     [PA]-x-[LIVSTG]  
ACT_SITE     38     38             K  
BINDING     17     17       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     [TS]  
BINDING     55     55       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     [DNCRHK]  
BINDING     116     116       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     E  
BINDING     42     42       /ligand="substrate     [ST]  
BINDING     55     55       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     [DN]  
BINDING     212     212       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     [ENQ]  

Additional information [?]

Size range 191-278 amino acids
Related rules None
Fusion Nter: MF_00835 (BioC); Cter: None