HAMAP logo
Due to maintenance work, this service will be unavailable from Mon Nov 11 17:30 until Tue Nov 12 09:00 CET. Apologies for the inconvenience.

HAMAP rule MF_00336

Send feedback

General rule information [?]

Accession MF_00336
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 43)
Name BioD
Scope(s) Bacteria
Archaea
Template(s) P13000 (BIOD1_ECOLI); P22818 (BIOD_LYSSH); P9WPQ5 (BIOD_MYCTU); O24872 (BIOD_HELPY); [ Recover all ]
Triggered by HAMAP; MF_00336 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier BIOD
Protein name RecName: Full=ATP-dependent dethiobiotin synthetase BioD;
                 EC=6.3.3.3;
AltName: Full=DTB synthetase;
                 Short=DTBS;
AltName: Full=Dethiobiotin synthase;
Gene name Name=bioD;

Comments [?]

FUNCTIONCatalyzes a mechanistically unusual reaction, the ATP- dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8- diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.
CATALYTIC ACTIVITY Reaction=(7R,8S)-7,8-diammoniononanoate + ATP + CO2 = (4R,5S)- dethiobiotin + ADP + 3 H(+) + phosphate; Xref=Rhea:RHEA:15805, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:149469, ChEBI:CHEBI:149473, ChEBI:CHEBI:456216; EC=6.3.3.3;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
PATHWAYCofactor biosynthesis; biotin biosynthesis; biotin from 7,8- diaminononanoate: step 1/2.
SUBUNITHomodimer.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the dethiobiotin synthetase family.

Keywords [?]

ATP-binding
Biotin biosynthesis
Cytoplasm
Ligase
Magnesium
Metal-binding
Nucleotide-binding

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0004141; Molecular function:dethiobiotin synthase activity
GO:0000287; Molecular function:magnesium ion binding
GO:0009102; Biological process:biotin biosynthetic process

Cross-references [?]

NCBIfam TIGR00347; BioD; 1;
PIRSF PIRSF006755; DTB_synth; 1;

Features [?]

From: BIOD1_ECOLI (P13000)
Key From To Description Tag Condition FTGroup
BINDING 13 18 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 [EDNSG]-[VAILSC]-G-K-[TS]-[VLTIYFH]
BINDING 116 119 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 E-x-x-G
BINDING 176 177 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 [NSMVILG]-[DEHRQTNCSMK]
BINDING 205 207 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 [PA]-x-[LIVSTG]
ACT_SITE 38 38 K
BINDING 17 17 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 [TS]
BINDING 55 55 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 [DNCRHK]
BINDING 116 116 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 E
BINDING 42 42 /ligand="substrate" [ST]
BINDING 55 55 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 [DN]
BINDING 212 212 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 [ENQ]

Additional information [?]

Size range 191-278 amino acids
Related rules None
Fusion Nter: MF_00835 (BioC) Cter: None



View rule in raw text format (no links)