HAMAP rule MF_00336

General rule information [?]

Accession	MF_00336
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 43)

Name

BioD

Scope

Bacteria

Archaea

Templates

P13000 (BIOD1_ECOLI); P22818 (BIOD_LYSSH); P9WPQ5 (BIOD_MYCTU); O24872 (BIOD_HELPY): [Recover all]

Triggered by

HAMAP; MF_00336 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

BIOD

Protein name

RecName:		Full=ATP-dependent dethiobiotin synthetase BioD;
		EC 6.3.3.3;
AltName:		Full=DTB synthetase;
		Short=DTBS;
AltName:		Full=Dethiobiotin synthase;

Gene name

bioD

Comments [?]

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.
Catalytic activity	RHEA:15805: (7R,8S)-7,8-diammoniononanoate + ATP + CO2 = (4R,5S)-dethiobiotin + ADP + 3 H(+) + phosphate EC 6.3.3.3
Cofactor	Mg(2+)
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.
Subunit	Homodimer.
Subcellular location	Cytoplasm.
Similarity	Belongs to the dethiobiotin synthetase family.

Keywords [?]

ATP-binding
Biotin biosynthesis
Cytoplasm
Ligase
Magnesium
Metal-binding
Nucleotide-binding

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004141; Molecular function: dethiobiotin synthase activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0009102; Biological process: biotin biosynthetic process.

Cross-references [?]

NCBIfam	TIGR00347; BioD; 1;
PIRSF	PIRSF006755; DTB_synth; 1;

Features [?]

From: BIOD1_ECOLI (P13000)

Key From To Description Tag Condition FTGroup

BINDING 13 18 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 [EDNSG]-[VAILSC]-G-K-[TS]-[VLTIYFH]

BINDING 116 119 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 E-x-x-G

BINDING 176 177 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 [NSMVILG]-[DEHRQTNCSMK]

BINDING 205 207 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 [PA]-x-[LIVSTG]

ACT_SITE 38 38 K

BINDING 17 17 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 [TS]

BINDING 55 55 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 [DNCRHK]

BINDING 116 116 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 E

BINDING 42 42 /ligand="substrate [ST]

BINDING 55 55 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 [DN]

BINDING 212 212 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 [ENQ]

Additional information [?]

Size range	191-278 amino acids
Related rules	None
Fusion	Nter: MF_00835 (BioC); Cter: None

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