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HAMAP rule MF_00336

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General rule information [?]

Accession MF_00336
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 43)
Name BioD
Scope(s) Bacteria
Template(s) P13000 (BIOD1_ECOLI); P22818 (BIOD_LYSSH); P9WPQ5 (BIOD_MYCTU); O24872 (BIOD_HELPY); [ Recover all ]
Triggered by HAMAP; MF_00336 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier BIOD
Protein name RecName: Full=ATP-dependent dethiobiotin synthetase BioD;
AltName: Full=DTB synthetase;
AltName: Full=Dethiobiotin synthase;
Gene name Name=bioD;

Comments [?]

FUNCTIONCatalyzes a mechanistically unusual reaction, the ATP- dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8- diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.
CATALYTIC ACTIVITY Reaction=(7R,8S)-7,8-diammoniononanoate + ATP + CO2 = (4R,5S)- dethiobiotin + ADP + 3 H(+) + phosphate; Xref=Rhea:RHEA:15805, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:149469, ChEBI:CHEBI:149473, ChEBI:CHEBI:456216; EC=;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
PATHWAYCofactor biosynthesis; biotin biosynthesis; biotin from 7,8- diaminononanoate: step 1/2.
SIMILARITYBelongs to the dethiobiotin synthetase family.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0004141; Molecular function:dethiobiotin synthase activity
GO:0000287; Molecular function:magnesium ion binding
GO:0009102; Biological process:biotin biosynthetic process

Cross-references [?]

NCBIfam TIGR00347; BioD; 1;
PIRSF PIRSF006755; DTB_synth; 1;

Features [?]

From: BIOD1_ECOLI (P13000)
Key From To Description Tag Condition FTGroup
BINDING 13 18 /ligand="ATP"
BINDING 116 119 /ligand="ATP"
BINDING 176 177 /ligand="ATP"
BINDING 205 207 /ligand="ATP"
ACT_SITE 38 38 K
BINDING 17 17 /ligand="Mg(2+)"
BINDING 55 55 /ligand="Mg(2+)"
BINDING 116 116 /ligand="Mg(2+)"
BINDING 42 42 /ligand="substrate" [ST]
BINDING 55 55 /ligand="ATP"
BINDING 212 212 /ligand="ATP"

Additional information [?]

Size range 191-278 amino acids
Related rules None
Fusion Nter: MF_00835 (BioC) Cter: None

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