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Annotation rule MF_00420
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General rule information [?]

Accession MF_00420
Dates 26-JUN-2001 (Created)
23-MAR-2020 (Last updated, Version 43)
Name PurL_2
Templates P12042 (PURL_BACSU); Q9X0X3 (PURL_THEMA); Q5SMH8 (PURL_THET8): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Phosphoribosylformylglycinamidine synthase subunit PurL;
Short=FGAM synthase;
AltName: Full=Formylglycinamide ribonucleotide amidotransferase subunit II;
Short=FGAR amidotransferase II;
AltName: Full=Phosphoribosylformylglycinamidine synthase subunit II;
AltName: Full=Glutamine amidotransferase PurL;
Gene name

Comments [?]

Function Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.
Catalytic activity RHEA:17129: ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate
Pathway Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.
Subunit Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.
Subcellular location Cytoplasm.
Similarity Belongs to the FGAMS family.

Keywords [?]

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0005524; Molecular function: ATP binding.
GO:0004642; Molecular function: phosphoribosylformylglycinamidine synthase activity.
GO:0006189; Biological process: 'de novo' IMP biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00586; AIRS; 1;
PF02769; AIRS_C; 1;
TIGRFAMs TIGR01736; FGAM_synth_II; 1;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
REGION     71     74       Substrate binding     S-H-N-H  
REGION     280     282       Substrate binding     E-S-Q  
ACT_SITE     32     32             H  
ACT_SITE     72     72       Proton acceptor     H  
METAL     70     70       Magnesium 1     E  
METAL     94     94       Magnesium 2     D  
METAL     236     236       Magnesium 2     D  
METAL     478     478       Magnesium 1     N  
BINDING     35     35       ATP     Y  
BINDING     68     68       ATP     [KR]  
BINDING     93     93       Substrate     R  
BINDING     189     189       Substrate; via carbonyl oxygen     R  
BINDING     208     208       Substrate     Q  
BINDING     442     442       ATP     [ND]  
BINDING     477     477       ATP; via amide nitrogen and carbonyl oxygen     G  
BINDING     480     480       Substrate     S  

Additional information [?]

Size range 603-824 amino acids
Related rules MF_00419 (PUR4)
Fusion None
Comments FGAM synthase can be composed of a single polypeptide (large PurL, MF_00419), or it can be composed of three separate proteins: PurL (small PurL, MF_00420), PurQ (MF_00421) and PurS (MF_01926).