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Annotation rule MF_00420 |
Accession | MF_00420 |
Dates | 26-JUN-2001 (Created) 23-MAR-2020 (Last updated, Version 43) |
Name | PurL_2 |
Scope | Bacteria
Archaea |
Templates | P12042 (PURL_BACSU); Q9X0X3 (PURL_THEMA); Q5SMH8 (PURL_THET8): [Recover all] |
Triggered by |
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Protein name |
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Gene name |
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Function | Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL. |
Catalytic activity | RHEA:17129: ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate
EC 6.3.5.3 |
Pathway | Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. |
Subunit | Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the FGAMS family. |
From: PURL_THEMA (Q9X0X3) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | 71 | 74 | Substrate binding | S-H-N-H | ||||||||
REGION | 280 | 282 | Substrate binding | E-S-Q | ||||||||
ACT_SITE | 32 | 32 | H | |||||||||
ACT_SITE | 72 | 72 | Proton acceptor | H | ||||||||
METAL | 70 | 70 | Magnesium 1 | E | ||||||||
METAL | 94 | 94 | Magnesium 2 | D | ||||||||
METAL | 236 | 236 | Magnesium 2 | D | ||||||||
METAL | 478 | 478 | Magnesium 1 | N | ||||||||
BINDING | 35 | 35 | ATP | Y | ||||||||
BINDING | 68 | 68 | ATP | [KR] | ||||||||
BINDING | 93 | 93 | Substrate | R | ||||||||
BINDING | 189 | 189 | Substrate; via carbonyl oxygen | R | ||||||||
BINDING | 208 | 208 | Substrate | Q | ||||||||
BINDING | 442 | 442 | ATP | [ND] | ||||||||
BINDING | 477 | 477 | ATP; via amide nitrogen and carbonyl oxygen | G | ||||||||
BINDING | 480 | 480 | Substrate | S |
Size range | 603-824 amino acids |
Related rules | MF_00419 (PUR4) |
Fusion | None |
Comments | FGAM synthase can be composed of a single polypeptide (large PurL, MF_00419), or it can be composed of three separate proteins: PurL (small PurL, MF_00420), PurQ (MF_00421) and PurS (MF_01926). |