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HAMAP rule MF_00420
General rule information
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Accession | MF_00420 |
Dates | 26-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 47) |
Name | PurL_2 |
Scope(s) |
Bacteria Archaea |
Template(s) | P12042 (PURL_BACSU); Q9X0X3 (PURL_THEMA); Q5SMH8 (PURL_THET8); [ Recover all ] |
Triggered by |
HAMAP; MF_00420 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | PURL |
Protein name | RecName: Full=Phosphoribosylformylglycinamidine synthase subunit PurL; Short=FGAM synthase; EC=6.3.5.3; AltName: Full=Formylglycinamide ribonucleotide amidotransferase subunit II; Short=FGAR amidotransferase II; Short=FGAR-AT II; AltName: Full=Phosphoribosylformylglycinamidine synthase subunit II; AltName: Full=Glutamine amidotransferase PurL; |
Gene name | Name=purL; |
Comments
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FUNCTION | Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP- dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL. |
CATALYTIC ACTIVITY | Reaction=ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D- ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D- ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate; Xref=Rhea:RHEA:17129, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:147286, ChEBI:CHEBI:147287, ChEBI:CHEBI:456216; EC=6.3.5.3; |
PATHWAY | Purine metabolism; IMP biosynthesis via de novo pathway; 5- amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5- phospho-D-ribosyl)glycinamide: step 1/2. |
SUBUNIT | Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits. |
SUBCELLULAR LOCATION | Cytoplasm. |
SIMILARITY | Belongs to the FGAMS family. |
Keywords
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Gene Ontology
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GO:0000287; Molecular function:magnesium ion binding |
GO:0005524; Molecular function:ATP binding |
GO:0004642; Molecular function:phosphoribosylformylglycinamidine synthase activity |
GO:0006189; Biological process:'de novo' IMP biosynthetic process |
GO:0005737; Cellular component:cytoplasm |
Cross-references
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Features
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From: PURL_THEMA (Q9X0X3) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 71 | 74 | /ligand="substrate" | S-H-N-H | ||||||||
BINDING | 280 | 282 | /ligand="substrate" | E-S-Q | ||||||||
ACT_SITE | 32 | 32 | H | |||||||||
ACT_SITE | 72 | 72 | /note="Proton acceptor" | H | ||||||||
BINDING | 70 | 70 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
E | ||||||||
BINDING | 94 | 94 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
D | ||||||||
BINDING | 236 | 236 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
D | ||||||||
BINDING | 478 | 478 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
N | ||||||||
BINDING | 35 | 35 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
Y | ||||||||
BINDING | 68 | 68 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[KR] | ||||||||
BINDING | 93 | 93 | /ligand="substrate" | R | ||||||||
BINDING | 189 | 189 | /ligand="substrate" | R | ||||||||
BINDING | 208 | 208 | /ligand="substrate" | Q | ||||||||
BINDING | 442 | 442 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[ND] | ||||||||
BINDING | 477 | 477 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G | ||||||||
BINDING | 480 | 480 | /ligand="substrate" | S |
Additional information
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Size range | 603-824 amino acids |
Related rules |
MF_00419 |
Fusion | Nter: None Cter: None |
Comments | FGAM synthase can be composed of a single polypeptide (large PurL, MF_00419), or it can be composed of three separate proteins: PurL (small PurL, MF_00420), PurQ (MF_00421) and PurS (MF_01926). |