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HAMAP rule MF_00421

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General rule information [?]

Accession MF_00421
Dates 26-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 44)
Name PurQ
Templates P12041 (PURQ_BACSU); Q9X0X2 (PURQ_THEMA): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Phosphoribosylformylglycinamidine synthase subunit PurQ;
Short=FGAM synthase;
AltName: Full=Formylglycinamide ribonucleotide amidotransferase subunit I;
Short=FGAR amidotransferase I;
Short=FGAR-AT I;
AltName: Full=Phosphoribosylformylglycinamidine synthase subunit I;
AltName: Full=Glutaminase PurQ;
Gene name

Comments [?]

Function Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.
Catalytic activity RHEA:17129: ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate
RHEA:15889: H2O + L-glutamine = L-glutamate + NH4(+)
Pathway Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.
Subunit Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.
Subcellular location Cytoplasm.

Keywords [?]

Gene Ontology [?]

GO:0004642; Molecular function: phosphoribosylformylglycinamidine synthase activity.
GO:0006189; Biological process: 'de novo' IMP biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS51273; GATASE_TYPE_1; 1; trigger=PRU00605;
NCBIfam TIGR01737; FGAM_synth_I; 1;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     86     86       Nucleophile     C  
ACT_SITE     186     186             H  
ACT_SITE     188     188             E  

Additional information [?]

Size range 212-272 amino acids
Related rules MF_00419 (PUR4)
Fusion None
Comments FGAM synthase can be composed of a single polypeptide (large PurL, MF_00419), or it can be composed of three separate proteins: PurL (small PurL, MF_00420), PurQ (MF_00421) and PurS (MF_01926).