HAMAP rule MF_00421

General rule information [?]

Accession	MF_00421
Dates	26-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 44)

Name

PurQ

Scope

Bacteria

Archaea

Templates

P12041 (PURQ_BACSU); Q9X0X2 (PURQ_THEMA): [Recover all]

Triggered by

HAMAP; MF_00421 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

PURQ

Protein name

RecName:		Full=Phosphoribosylformylglycinamidine synthase subunit PurQ;
		Short=FGAM synthase;
		EC 6.3.5.3;
AltName:		Full=Formylglycinamide ribonucleotide amidotransferase subunit I;
		Short=FGAR amidotransferase I;
		Short=FGAR-AT I;
AltName:		Full=Phosphoribosylformylglycinamidine synthase subunit I;
AltName:		Full=Glutaminase PurQ;
		EC 3.5.1.2;

Gene name

purQ

Comments [?]

Function	Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.
Catalytic activity	RHEA:17129: ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate EC 6.3.5.3
	RHEA:15889: H2O + L-glutamine = L-glutamate + NH4(+) EC 3.5.1.2
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.
Subunit	Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.
Subcellular location	Cytoplasm.

Keywords [?]

ATP-binding
Cytoplasm
Glutamine amidotransferase
Hydrolase
Ligase
Nucleotide-binding
Purine biosynthesis

Gene Ontology [?]

GO:0004642; Molecular function: phosphoribosylformylglycinamidine synthase activity.
GO:0006189; Biological process: 'de novo' IMP biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE	PS51273; GATASE_TYPE_1; 1; trigger=PRU00605;
NCBIfam	TIGR01737; FGAM_synth_I; 1;

Features [?]

From: PURQ_THEMA (Q9X0X2)

Key From To Description Tag Condition FTGroup

ACT_SITE 86 86 Nucleophile C

ACT_SITE 186 186 H

ACT_SITE 188 188 E

Additional information [?]

Size range	212-272 amino acids
Related rules	MF_00419 (PUR4)
Fusion	None
Comments	FGAM synthase can be composed of a single polypeptide (large PurL, MF_00419), or it can be composed of three separate proteins: PurL (small PurL, MF_00420), PurQ (MF_00421) and PurS (MF_01926).

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