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HAMAP rule MF_00421

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General rule information [?]

Accession MF_00421
Dates 26-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 44)
Name PurQ
Scope(s) Bacteria
Template(s) P12041 (PURQ_BACSU); Q9X0X2 (PURQ_THEMA); [ Recover all ]
Triggered by HAMAP; MF_00421 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PURQ
Protein name RecName: Full=Phosphoribosylformylglycinamidine synthase subunit PurQ;
                 Short=FGAM synthase;
AltName: Full=Formylglycinamide ribonucleotide amidotransferase subunit I;
                 Short=FGAR amidotransferase I;
                 Short=FGAR-AT I;
AltName: Full=Phosphoribosylformylglycinamidine synthase subunit I;
AltName: Full=Glutaminase PurQ;
Gene name Name=purQ;

Comments [?]

FUNCTIONPart of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP- dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.
CATALYTIC ACTIVITY Reaction=ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D- ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D- ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate; Xref=Rhea:RHEA:17129, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:147286, ChEBI:CHEBI:147287, ChEBI:CHEBI:456216; EC=;
CATALYTIC ACTIVITY Reaction=H2O + L-glutamine = L-glutamate + NH4(+); Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=;
PATHWAYPurine metabolism; IMP biosynthesis via de novo pathway; 5- amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5- phospho-D-ribosyl)glycinamide: step 1/2.
SUBUNITPart of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.

Keywords [?]

Gene Ontology [?]

GO:0004642; Molecular function:phosphoribosylformylglycinamidine synthase activity
GO:0006189; Biological process:'de novo' IMP biosynthetic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

NCBIfam TIGR01737; FGAM_synth_I; 1;

Features [?]

Key From To Description Tag Condition FTGroup
ACT_SITE 86 86 /note="Nucleophile" C
ACT_SITE 186 186 H
ACT_SITE 188 188 E

Additional information [?]

Size range 212-272 amino acids
Related rules MF_00419
Fusion Nter: None Cter: None
Comments FGAM synthase can be composed of a single polypeptide (large PurL, MF_00419), or it can be composed of three separate proteins: PurL (small PurL, MF_00420), PurQ (MF_00421) and PurS (MF_01926).

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