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HAMAP rule MF_00443

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General rule information [?]

Accession MF_00443
Dates 28-AUG-2001 (Created)
19-NOV-2022 (Last updated, Version 39)
Name ThiG
Scope(s) Bacteria
Template(s) O31618 (THIG_BACSU); P30139 (THIG_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00443 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier THIG
Protein name RecName: Full=Thiazole synthase;
Gene name Name=thiG;

Comments [?]

FUNCTIONCatalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S.
CATALYTIC ACTIVITY Reaction=1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + [sulfur- carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH = 2-[(2R,5Z)-2- carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur- carrier protein ThiS]-C-terminal Gly-Gly + 2 H(+) + 2 H2O; Xref=Rhea:RHEA:26297, Rhea:RHEA-COMP:12908, Rhea:RHEA-COMP:12909, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57792, ChEBI:CHEBI:62899, ChEBI:CHEBI:77846, ChEBI:CHEBI:90619, ChEBI:CHEBI:90778; EC=;
PATHWAYCofactor biosynthesis; thiamine diphosphate biosynthesis.
SUBUNITHomotetramer. Forms heterodimers with either ThiH or ThiS.
case <OG:Chloroplast>
SUBCELLULAR LOCATIONPlastid, chloroplast.
else case not <OG:Chloroplast>
end case
SIMILARITYBelongs to the ThiG family.

Keywords [?]

case not <OG:Chloroplast>
end case
Schiff base
Thiamine biosynthesis

Gene Ontology [?]

GO:0016783; Molecular function:sulfurtransferase activity
GO:0009228; Biological process:thiamine biosynthetic process
case <OG:Chloroplast>
GO:0009507; Cellular component:chloroplast
else case not <OG:Chloroplast>
GO:0005737; Cellular component:cytoplasm
end case

Cross-references [?]

Pfam PF05690; ThiG; 1;

Features [?]

From: THIG_BACSU (O31618)
Key From To Description Tag Condition FTGroup
BINDING 185 186 /ligand="1-deoxy-D-xylulose 5-phosphate"
BINDING 207 208 /ligand="1-deoxy-D-xylulose 5-phosphate"
ACT_SITE 98 98 /note="Schiff-base intermediate with DXP" K
BINDING 159 159 /ligand="1-deoxy-D-xylulose 5-phosphate"

Additional information [?]

Size range 252-337 amino acids
Related rules None
Fusion Nter: <thiO>; <thiS> Cter: None
Comments ThiO N-terminal domain (glycine oxidase) in NOSS1, TRIV2, and SYNY3. Possible wrong start in CYACA.

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