 |
|
Annotation rule MF_00443 |
General rule information
[?]
Accession |
MF_00443 |
Dates |
28-AUG-2001 (Created) 18-NOV-2019 (Last updated, Version 35) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Function |
Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S. |
Catalytic activity |
RHEA:26297: 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH = 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS]-C-terminal Gly-Gly + 2 H(+) + 2 H2O
EC 2.8.1.10
|
Pathway |
Cofactor biosynthesis; thiamine diphosphate biosynthesis. |
Subunit |
Homotetramer. Forms heterodimers with either ThiH or ThiS. |
case <OG:Chloroplast>
Subcellular location |
Plastid, chloroplast. |
else case not <OG:Chloroplast>
Subcellular location |
Cytoplasm. |
end case
Similarity |
Belongs to the ThiG family. |
case not <OG:Chloroplast>
end case
GO:0016783; Molecular function: sulfurtransferase activity.
GO:0009228; Biological process: thiamine biosynthetic process.
case <OG:Chloroplast>
else case not <OG:Chloroplast>
end case
From: THIG_BACSU (O31618) |
Key
|
|
From
|
|
To
|
|
Description
|
|
Tag
|
|
Condition
|
|
FTGroup
|
REGION
|
|
185
|
|
186
|
|
DXP binding
|
|
|
|
A-G
|
|
|
REGION
|
|
207
|
|
208
|
|
DXP binding
|
|
|
|
x-[TS]
|
|
|
ACT_SITE
|
|
98
|
|
98
|
|
Schiff-base intermediate with DXP
|
|
|
|
K
|
|
|
BINDING
|
|
159
|
|
159
|
|
DXP; via amide nitrogen
|
|
|
|
G
|
|
|
Additional information
[?]
Size range |
252-337 amino acids |
Related rules |
None |
Fusion |
Nter: <thiO>, <thiS>; Cter: None |
Comments |
ThiO N-terminal domain (glycine oxidase) in NOSS1, TRIV2, and SYNY3. Possible wrong start in CYACA. |