HAMAP rule MF_00443
General rule information
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| Accession | MF_00443 |
| Dates | 28-AUG-2001 (Created)
3-SEP-2024 (Last updated, Version 41) |
| Name | ThiG |
| Scope(s) |
Bacteria Plastid |
| Template(s) | O31618 (THIG_BACSU); P30139 (THIG_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00443 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | THIG |
| Protein name | RecName: Full=Thiazole synthase; EC=2.8.1.10; |
| Gene name | Name=thiG; |
Comments
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| FUNCTION | Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S. |
| CATALYTIC ACTIVITY | Reaction=[sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + 2-iminoacetate + 1-deoxy-D-xylulose 5-phosphate = [sulfur-carrier protein ThiS]-C-terminal Gly-Gly + 2-[(2R,5Z)-2-carboxy-4- methylthiazol-5(2H)-ylidene]ethyl phosphate + 2 H2O + 2 H(+); Xref=Rhea:RHEA:26297, Rhea:RHEA-COMP:12908, Rhea:RHEA-COMP:12909, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57792, ChEBI:CHEBI:62899, ChEBI:CHEBI:77846, ChEBI:CHEBI:90619, ChEBI:CHEBI:90778; EC=2.8.1.10; |
| PATHWAY | Cofactor biosynthesis; thiamine diphosphate biosynthesis. |
| SUBUNIT | Homotetramer. Forms heterodimers with either ThiH or ThiS. |
| case <OG:Chloroplast> | |
| SUBCELLULAR LOCATION | Plastid, chloroplast. |
| else case not <OG:Chloroplast> | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| end case | |
| SIMILARITY | Belongs to the ThiG family. |
Keywords
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| case not <OG:Chloroplast> | |
| Cytoplasm | |
| end case | |
| Schiff base | |
| Thiamine biosynthesis | |
| Transferase | |
Gene Ontology
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| GO:0016783; Molecular function:sulfurtransferase activity | |
| GO:0009229; Biological process:thiamine diphosphate biosynthetic process | |
| case <OG:Chloroplast> | |
| GO:0009507; Cellular component:chloroplast | |
| else case not <OG:Chloroplast> | |
| GO:0005737; Cellular component:cytoplasm | |
| end case | |
Cross-references
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| Pfam | PF05690; ThiG; 1; |
Features
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| From: THIG_BACSU (O31618) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 185 | 186 | /ligand="1-deoxy-D-xylulose 5-phosphate" /ligand_id="ChEBI:CHEBI:57792" |
A-G | ||||||||
| BINDING | 207 | 208 | /ligand="1-deoxy-D-xylulose 5-phosphate" /ligand_id="ChEBI:CHEBI:57792" |
x-[TS] | ||||||||
| ACT_SITE | 98 | 98 | /note="Schiff-base intermediate with DXP" | K | ||||||||
| BINDING | 159 | 159 | /ligand="1-deoxy-D-xylulose 5-phosphate" /ligand_id="ChEBI:CHEBI:57792" |
G | ||||||||
Additional information
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| Size range | 252-337 amino acids |
| Related rules |
None |
| Fusion | Nter: <thiO>; <thiS> Cter: None |
| Comments | ThiO N-terminal domain (glycine oxidase) in NOSS1, TRIV2, and SYNY3. Possible wrong start in CYACA. |