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HAMAP rule MF_00445

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General rule information [?]

Accession MF_00445
Dates 31-AUG-2001 (Created)
15-JUN-2023 (Last updated, Version 60)
Name NDH1_NuoN_1
Scope(s) Bacteria
Plastid
Template(s) P0AFF0 (NUON_ECOLI); A1B479 (NUON_PARDP); Q56229 (NQO14_THET8); [ Recover all ]
Triggered by HAMAP; MF_00445 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OG:Chloroplast>
Identifier NU2C
Protein name RecName: Full=NAD(P)H-quinone oxidoreductase subunit 2, chloroplastic;
                 EC=7.1.1.-;
AltName: Full=NAD(P)H dehydrogenase, subunit 2;
AltName: Full=NADH-plastoquinone oxidoreductase subunit 2;
Gene name Name=ndhB;
else case <OC:Cyanobacteriota>
Identifier NU2C
Protein name RecName: Full=NAD(P)H-quinone oxidoreductase subunit 2;
                 EC=7.1.1.-;
AltName: Full=NAD(P)H dehydrogenase subunit 2;
AltName: Full=NADH-plastoquinone oxidoreductase subunit 2;
AltName: Full=NDH-1, subunit 2;
Gene name Name=ndhB;
else
Identifier NUON
Protein name RecName: Full=NADH-quinone oxidoreductase subunit N;
                 EC=7.1.1.-;
AltName: Full=NADH dehydrogenase I subunit N;
AltName: Full=NDH-1 subunit N;
Gene name Name=nuoN;
end case

Comments [?]

case <OG:Chloroplast>
FUNCTIONNDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient.
else case <OC:Cyanobacteriota>
FUNCTIONNDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient. Cyanobacterial NDH-1 also plays a role in inorganic carbon-concentration.
else case <OC:Actinomycetota> or <OC:Bacteroidota> or <OC:Chlorobiota> or <OC:Deinococcota> or <OC:Bacillota>
FUNCTIONNDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
else
FUNCTIONNDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
end case
case <OG:Chloroplast> or <OC:Cyanobacteriota>
CATALYTIC ACTIVITY Reaction=a plastoquinone + (n+1) H(+)(in) + NADH = a plastoquinol + n H(+)(out) + NAD(+); Xref=Rhea:RHEA:42608, Rhea:RHEA-COMP:9561, Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:62192;
CATALYTIC ACTIVITY Reaction=a plastoquinone + (n+1) H(+)(in) + NADPH = a plastoquinol + n H(+)(out) + NADP(+); Xref=Rhea:RHEA:42612, Rhea:RHEA-COMP:9561, Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:62192;
else
CATALYTIC ACTIVITY Reaction=a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124;
end case
case <OG:Chloroplast>
SUBUNITNDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus.
else case <OC:Cyanobacteriota>
SUBUNITNDH-1 can be composed of about 15 different subunits; different subcomplexes with different compositions have been identified which probably have different functions.
else case <OC:Enterobacterales> or <OC:Shewanellaceae> or <OC:Pseudomonadaceae>
SUBUNITNDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.
else case <OC:Deinococci>
SUBUNITNDH-1 is composed of 15 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.
else
SUBUNITNDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.
end case
case <OG:Chloroplast>
SUBCELLULAR LOCATIONPlastid, chloroplast thylakoid membrane; Multi- pass membrane protein.
else case <OC:Cyanobacteriota> and not <OC:Gloeobacter>
SUBCELLULAR LOCATIONCellular thylakoid membrane; Multi-pass membrane protein.
else case <OC:Gloeobacter>
SUBCELLULAR LOCATIONCell inner membrane; Multi-pass membrane protein.
else case not defined <Property:Membrane> or <Property:Membrane=1>
SUBCELLULAR LOCATIONCell membrane; Multi-pass membrane protein.
else case <Property:Membrane=2>
SUBCELLULAR LOCATIONCell inner membrane; Multi-pass membrane protein.
end case
SIMILARITYBelongs to the complex I subunit 2 family.

Keywords [?]

NAD
case <OG:Chloroplast> or <OC:Cyanobacteriota>
NADP
Plastoquinone
else case not <OC:Actinomycetota> and not <OC:Bacteroidota> and not <OC:Chlorobiota> and not <OC:Deinococcota> and not <OC:Bacillota>
Ubiquinone
end case
Quinone
Membrane
Transmembrane
Transport
case <OG:Chloroplast> or <OC:Cyanobacteriota> and not <OC:Gloeobacter>
Thylakoid
else case <OC:Gloeobacter>
Cell membrane
Cell inner membrane
else case not defined <Property:Membrane> or <Property:Membrane=1>
Cell membrane
else case <Property:Membrane=2>
Cell membrane
Cell inner membrane
end case
Transmembrane helix
<a href="https://www.uniprot.org/keywords/KW-1278">Translocase</a>

Gene Ontology [?]

case <OG:Chloroplast> or <OCellular component:Cyanobacteriota>
GO:0016655; Molecular function:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
GO:0019684; Biological process:photosynthesis, light reaction
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0050136; Molecular function:NADH dehydrogenase (quinone) activity
end case
case <OCellular component:Cyanobacteriota> and not <OC:Gloeobacter>
GO:0042651; Cellular component:thylakoid membrane
else case <OG:Chloroplast>
GO:0009535; Cellular component:chloroplast thylakoid membrane
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0005886; Cellular component:plasma membrane
end case

Cross-references [?]

Pfam PF00361; Oxidored_q1; 1;
PRINTS PR01434; NADHDHGNASE5; 1;
NCBIfam TIGR01770; NDH_I_N; 1;
General Transmembrane; -; 11-15;

Features [?]


Additional information [?]

Size range 427-554 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments 14 proteins form the NDH-1 complex in most bacteria, there are at least 15 subunits in cyanobacteria and chloroplasts. PARDE and THET8 are annotated with another nomenclature. Many higher plant ndhB transcripts undergo RNA editing. The gene is often present in the inverted repeat of higher plant plastid genomes. Was originally constructed as 2 families, MF_00445 and MF_01439 which were merged.



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