HAMAP rule MF_00445
General rule information
[?]
| Accession | MF_00445 |
| Dates | 31-AUG-2001 (Created)
3-SEP-2024 (Last updated, Version 62) |
| Name | NDH1_NuoN_1 |
| Scope(s) |
Bacteria Plastid |
| Template(s) | P0AFF0 (NUON_ECOLI); A1B479 (NUON_PARDP); Q56229 (NQO14_THET8); [ Recover all ] |
| Triggered by |
HAMAP; MF_00445 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| case <OG:Chloroplast> | |
| Identifier | NU2C |
| Protein name | RecName: Full=NAD(P)H-quinone oxidoreductase subunit 2, chloroplastic; EC=7.1.1.-; AltName: Full=NAD(P)H dehydrogenase, subunit 2; AltName: Full=NADH-plastoquinone oxidoreductase subunit 2; |
| Gene name | Name=ndhB; |
| else case <OC:Cyanobacteriota> | |
| Identifier | NU2C |
| Protein name | RecName: Full=NAD(P)H-quinone oxidoreductase subunit 2; EC=7.1.1.-; AltName: Full=NAD(P)H dehydrogenase subunit 2; AltName: Full=NADH-plastoquinone oxidoreductase subunit 2; AltName: Full=NDH-1, subunit 2; |
| Gene name | Name=ndhB; |
| else | |
| Identifier | NUON |
| Protein name | RecName: Full=NADH-quinone oxidoreductase subunit N; EC=7.1.1.-; AltName: Full=NADH dehydrogenase I subunit N; AltName: Full=NDH-1 subunit N; |
| Gene name | Name=nuoN; |
| end case | |
Comments
[?]
| case <OG:Chloroplast> | |
| FUNCTION | NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient. |
| else case <OC:Cyanobacteriota> | |
| FUNCTION | NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient. Cyanobacterial NDH-1 also plays a role in inorganic carbon-concentration. |
| else case <OC:Actinomycetota> or <OC:Bacteroidota> or <OC:Chlorobiota> or <OC:Deinococcota> or <OC:Bacillota> | |
| FUNCTION | NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. |
| else | |
| FUNCTION | NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. |
| end case | |
| case <OG:Chloroplast> or <OC:Cyanobacteriota> | |
| CATALYTIC ACTIVITY | Reaction=a plastoquinone + NADH + (n+1) H(+)(in) = a plastoquinol + NAD(+) + n H(+)(out); Xref=Rhea:RHEA:42608, Rhea:RHEA-COMP:9561, Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:62192; |
| CATALYTIC ACTIVITY | Reaction=a plastoquinone + NADPH + (n+1) H(+)(in) = a plastoquinol + NADP(+) + n H(+)(out); Xref=Rhea:RHEA:42612, Rhea:RHEA-COMP:9561, Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:62192; |
| else | |
| CATALYTIC ACTIVITY | Reaction=a quinone + NADH + 5 H(+)(in) = a quinol + NAD(+) + 4 H(+)(out); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124; |
| end case | |
| case <OG:Chloroplast> | |
| SUBUNIT | NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus. |
| else case <OC:Cyanobacteriota> | |
| SUBUNIT | NDH-1 can be composed of about 15 different subunits; different subcomplexes with different compositions have been identified which probably have different functions. |
| else case <OC:Enterobacterales> or <OC:Shewanellaceae> or <OC:Pseudomonadaceae> | |
| SUBUNIT | NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex. |
| else case <OC:Deinococci> | |
| SUBUNIT | NDH-1 is composed of 15 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex. |
| else | |
| SUBUNIT | NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex. |
| end case | |
| case <OG:Chloroplast> | |
| SUBCELLULAR LOCATION | Plastid, chloroplast thylakoid membrane; Multi- pass membrane protein. |
| else case <OC:Cyanobacteriota> and not <OC:Gloeobacter> | |
| SUBCELLULAR LOCATION | Cellular thylakoid membrane; Multi-pass membrane protein. |
| else case <OC:Gloeobacter> | |
| SUBCELLULAR LOCATION | Cell inner membrane; Multi-pass membrane protein. |
| else case not defined <Property:Membrane> or <Property:Membrane=1> | |
| SUBCELLULAR LOCATION | Cell membrane; Multi-pass membrane protein. |
| else case <Property:Membrane=2> | |
| SUBCELLULAR LOCATION | Cell inner membrane; Multi-pass membrane protein. |
| end case | |
| SIMILARITY | Belongs to the complex I subunit 2 family. |
Keywords
[?]
| NAD | |
| case <OG:Chloroplast> or <OC:Cyanobacteriota> | |
| NADP | |
| Plastoquinone | |
| else case not <OC:Actinomycetota> and not <OC:Bacteroidota> and not <OC:Chlorobiota> and not <OC:Deinococcota> and not <OC:Bacillota> | |
| Ubiquinone | |
| end case | |
| Quinone | |
| Membrane | |
| Transmembrane | |
| Transport | |
| case <OG:Chloroplast> or <OC:Cyanobacteriota> and not <OC:Gloeobacter> | |
| Thylakoid | |
| else case <OC:Gloeobacter> | |
| Cell membrane | |
| Cell inner membrane | |
| else case not defined <Property:Membrane> or <Property:Membrane=1> | |
| Cell membrane | |
| else case <Property:Membrane=2> | |
| Cell membrane | |
| Cell inner membrane | |
| end case | |
| Transmembrane helix | |
| <a href="https://www.uniprot.org/keywords/KW-1278">Translocase</a> | |
Gene Ontology
[?]
| case <OG:Chloroplast> or <OCellular component:Cyanobacteriota> | |
| GO:0016655; Molecular function:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor | |
| GO:0019684; Biological process:photosynthesis, light reaction | |
| else; https://www.ebi.ac.uk/QuickGO/term/else | |
| GO:0050136; Molecular function:NADH:ubiquinone reductase (non-electrogenic) activity | |
| end case | |
| case <OCellular component:Cyanobacteriota> and not <OC:Gloeobacter> | |
| GO:0042651; Cellular component:thylakoid membrane | |
| else case <OG:Chloroplast> | |
| GO:0009535; Cellular component:chloroplast thylakoid membrane | |
| else; https://www.ebi.ac.uk/QuickGO/term/else | |
| GO:0005886; Cellular component:plasma membrane | |
| end case | |
Cross-references
[?]
| Pfam | PF00361; Oxidored_q1; 1; |
| PRINTS | PR01434; NADHDHGNASE5; 1; |
| NCBIfam | TIGR01770; NDH_I_N; 1; |
| General | Transmembrane; -; 11-15; |
Features
[?]
Additional information
[?]
| Size range | 427-554 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | 14 proteins form the NDH-1 complex in most bacteria, there are at least 15 subunits in cyanobacteria and chloroplasts. PARDE and THET8 are annotated with another nomenclature. Many higher plant ndhB transcripts undergo RNA editing. The gene is often present in the inverted repeat of higher plant plastid genomes. Was originally constructed as 2 families, MF_00445 and MF_01439 which were merged. |