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Annotation rule MF_00536 |
General rule information
[?]
Accession |
MF_00536 |
Dates |
30-JAN-2002 (Created) 18-NOV-2019 (Last updated, Version 32) |
Propagated annotation
[?]
Identifier, protein and gene names
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Protein name |
RecName:
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Full=4-hydroxythreonine-4-phosphate dehydrogenase;
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EC 1.1.1.262;
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AltName:
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Full=4-(phosphohydroxy)-L-threonine dehydrogenase;
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Function |
Catalyzes the NAD(P)-dependent oxidation of 4-(phosphooxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphooxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP). |
Catalytic activity |
RHEA:32275: 4-(phosphooxy)-L-threonine + NAD(+) = 3-amino-2-oxopropyl phosphate + CO2 + NADH
EC 1.1.1.262
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case <OC:Proteobacteria>
Cofactor |
Zn(2+) Mg(2+) Co(2+) Note: Binds 1 divalent metal cation per subunit. Can use ions such as Zn(2+), Mg(2+) or Co(2+). |
else
end case
Pathway |
Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 4/5. |
Subunit |
Homodimer. |
Subcellular location |
Cytoplasm. |
Miscellaneous |
The active site is located at the dimer interface. |
Similarity |
Belongs to the PdxA family. |
case <OC:Proteobacteria>
end case
GO:0050570; Molecular function: 4-hydroxythreonine-4-phosphate dehydrogenase activity.
GO:0046872; Molecular function: metal ion binding.
GO:0042823; Biological process: pyridoxal phosphate biosynthetic process.
GO:0008615; Biological process: pyridoxine biosynthetic process.
case <OC:Proteobacteria>
GO:0050897; Molecular function: cobalt ion binding.
GO:0008270; Molecular function: zinc ion binding.
GO:0000287; Molecular function: magnesium ion binding.
end case
From: PDXA_ECOLI (P19624) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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METAL
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166
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166
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Divalent metal cation; shared with dimeric partner
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H
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METAL
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211
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211
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Divalent metal cation; shared with dimeric partner
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H
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METAL
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266
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266
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Divalent metal cation; shared with dimeric partner
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H
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BINDING (Optional)
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136
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136
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Substrate
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H
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BINDING (Optional)
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137
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137
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Substrate
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[TS]
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BINDING
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274
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274
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Substrate
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K
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BINDING (Optional)
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283
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283
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Substrate
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N
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BINDING (Optional)
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292
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292
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Substrate
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R
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Additional information
[?]
Size range |
307-364 amino acids |
Related rules |
None |
Fusion |
None |
Comments |
MF_00536 signature has been revised (March 2017) to avoid wrong annotation of DUF1537-linked PdxA2, which are paralogs of PdxA and are involved in carbon source metabolism rather than PLP biosynthesis. |