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HAMAP rule MF_00558

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General rule information [?]

Accession MF_00558
Dates 23-MAY-2002 (Created)
1-JUN-2023 (Last updated, Version 37)
Name Succ_CoA_beta
Templates P0A836 (SUCC_ECOLI); P25126 (SUCC_THETH); P80886 (SUCC_BACSU); P53590 (SUCB2_PIG): [Recover all]
case <OC:Bacteria> or <OC:Archaea>
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Succinate--CoA ligase [ADP-forming] subunit beta;
AltName: Full=Succinyl-CoA synthetase subunit beta;
Gene name

Comments [?]

Function Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
Catalytic activity RHEA:17661: ATP + CoA + succinate = ADP + phosphate + succinyl-CoA
PhysiologicalDirection=right-to-left (RHEA:17663)
RHEA:22120: CoA + GTP + succinate = GDP + phosphate + succinyl-CoA
PhysiologicalDirection=right-to-left (RHEA:22122)
case <FTGroup:1>
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
end case
Pathway Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
Subunit Heterotetramer of two alpha and two beta subunits.
Similarity Belongs to the succinate/malate CoA ligase beta subunit family.

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
GO:0004775; Molecular function: succinate-CoA ligase (ADP-forming) activity.
GO:0006099; Biological process: tricarboxylic acid cycle.

Cross-references [?]

PS50975; ATP_GRASP; 1;
Pfam PF08442; ATP-grasp_2; 1;
PF00549; Ligase_CoA; 1;
NCBIfam TIGR01016; SucCoAbeta; 1;
PIRSF PIRSF001554; SucCS_beta; 1;

Features [?]

case <Feature:PS50975>
From: SUCC_ECOLI (P0A836)
Key     From     To       Description   Tag   Condition   FTGroup
DOMAIN     9     244       ATP-grasp        
end case
BINDING     53     55       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     G-R-G  
BINDING     321     323       /ligand="substrate" /ligand_note="ligand shared with subunit alpha     G-I-[VAMTL]  
BINDING     199     199       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     N   1
BINDING     213     213       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     D   1
BINDING     46     46       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     K  
BINDING (Optional)     99     99       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     E  
BINDING     102     102       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616        
BINDING     107     107       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     E  
BINDING     264     264       /ligand="substrate" /ligand_note="ligand shared with subunit alpha     N  

Additional information [?]

Size range 364-399 amino acids
Related rules MF_03220 (SUCB1 supersedes the current rule); MF_03221 (SUCB2 supersedes the current rule)
Fusion None
Comments It is not possible to automatically make a distinction between a bona-fide sucC and mtkA (not shown in alignment).