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HAMAP rule MF_00558

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General rule information [?]

Accession MF_00558
Dates 23-MAY-2002 (Created)
1-JUN-2023 (Last updated, Version 37)
Name Succ_CoA_beta
Scope(s) Bacteria
Archaea
Template(s) P0A836 (SUCC_ECOLI); P25126 (SUCC_THETH); P80886 (SUCC_BACSU); P53590 (SUCB2_PIG); [ Recover all ]
Triggered by
case c? <OC:Bacteria> or <OC:Archaea>
HAMAP; MF_00558 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SUCC
Protein name RecName: Full=Succinate--CoA ligase [ADP-forming] subunit beta;
                 EC=6.2.1.5;
AltName: Full=Succinyl-CoA synthetase subunit beta;
                 Short=SCS-beta;
Gene name Name=sucC;

Comments [?]

FUNCTIONSuccinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
CATALYTIC ACTIVITY Reaction=ATP + CoA + succinate = ADP + phosphate + succinyl-CoA; Xref=Rhea:RHEA:17661, ChEBI:CHEBI:30031, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292, ChEBI:CHEBI:456216; EC=6.2.1.5; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:17663;
CATALYTIC ACTIVITY Reaction=CoA + GTP + succinate = GDP + phosphate + succinyl-CoA; Xref=Rhea:RHEA:22120, ChEBI:CHEBI:30031, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292, ChEBI:CHEBI:58189; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:22122;
case <FTGroup:1>
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
end case
PATHWAYCarbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
SUBUNITHeterotetramer of two alpha and two beta subunits.
SIMILARITYBelongs to the succinate/malate CoA ligase beta subunit family.

Keywords [?]

ATP-binding
Ligase
case <FTGroup:1>
Magnesium
Metal-binding
end case
Nucleotide-binding
Tricarboxylic acid cycle

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
case <FTGroup:1>
GO:0000287; Molecular function:magnesium ion binding
end case
GO:0004775; Molecular function:succinate-CoA ligase (ADP-forming) activity
GO:0006099; Biological process:tricarboxylic acid cycle

Cross-references [?]

PROSITE PS01217; SUCCINYL_COA_LIG_3; 1;
PROSITE PS50975; ATP_GRASP; 1;
Pfam PF08442; ATP-grasp_2; 1;
Pfam PF00549; Ligase_CoA; 1;
NCBIfam TIGR01016; SucCoAbeta; 1;
PIRSF PIRSF001554; SucCS_beta; 1;

Features [?]

From: SUCC_ECOLI (P0A836)
Key From To Description Tag Condition FTGroup
case <Feature:PS50975>
DOMAIN 9 244 /note="ATP-grasp"
end case
BINDING 53 55 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 G-R-G
BINDING 321 323 /ligand="substrate"
/ligand_note="ligand shared with subunit alpha"		 G-I-[VAMTL]
BINDING 199 199 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 N 1
BINDING 213 213 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 D 1
BINDING 46 46 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 K
BINDING 99 99 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 E
BINDING 102 102 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
BINDING 107 107 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 E
BINDING 264 264 /ligand="substrate"
/ligand_note="ligand shared with subunit alpha"		 N

Additional information [?]

Size range 364-399 amino acids
Related rules MF_03220
MF_03221
Fusion Nter: None Cter: None
Comments It is not possible to automatically make a distinction between a bona-fide sucC and mtkA (not shown in alignment).



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