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HAMAP rule MF_00587

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General rule information [?]

Accession MF_00587
Dates 22-OCT-2002 (Created)
19-NOV-2022 (Last updated, Version 28)
Name tRNA_methyltr_TrmY
Scope
Bacteria; Gammaproteobacteria
Archaea
Templates Q59034 (TRMY_METJA); D4GTL8 (TRMY_HALVD): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

case <OC:Archaea>
Identifier
TRMY
Protein name
RecName: Full=tRNA (pseudouridine(54)-N(1))-methyltransferase;
EC 2.1.1.257;
Gene name
trmY
else case <OC:Bacteria>
Identifier
TRMYL
Protein name
RecName: Full=Putative pseudouridine methyltransferase;
EC 2.1.1.-;
end case

Comments [?]

case <OC:Archaea>
Function Specifically catalyzes the N1-methylation of pseudouridine at position 54 (Psi54) in tRNAs.
Catalytic activity RHEA:55292: pseudouridine(54) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylpseudouridine(54) in tRNA + S-adenosyl-L-homocysteine
EC 2.1.1.257
Subunit Homodimer.
end case
Subcellular location Cytoplasm.
Similarity Belongs to the methyltransferase superfamily. TrmY family.

Keywords [?]

Cytoplasm
Methyltransferase
S-adenosyl-L-methionine
Transferase
case <OC:Archaea>
tRNA processing
end case

Gene Ontology [?]

case <OC:Archaea>
GO:0030488; Biological process: tRNA methylation.
GO:0008175; Molecular function: tRNA methyltransferase activity.
end case
GO:0008757; Molecular function: S-adenosylmethionine-dependent methyltransferase activity.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF04013; Methyltrn_RNA_2; 1;

Features [?]

From: TRMY_METJA (Q59034)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING (Optional)     179     184       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [LIV]-S-P-L-[ES]-L  
BINDING (Optional)     136     136       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [LM]  
BINDING (Optional)     156     156       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     G  
BINDING (Optional)     189     189       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     C  

Additional information [?]

Size range 192-211 amino acids
Related rules None
Fusion Nter: <Unknown>; Cter: None
Comments Formation of 1-methylpseudouridine at position 54 of tRNA has never been identified in any bacterial tRNA sequenced so far. The bacterial and archaeal homologs may fulfill different functions. See PubMed:22274953. Unknown N-terminal domain in ARCFU. Divergent PYRAE not shown in alignment and not used in size range.


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