HAMAP rule MF_00587
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00587 |
| Accession | MF_00587 |
| Dates | 28-FEB-2005 (Created)
03-SEP-2024 (Last updated, Version 23) |
| Name | tRNA_methyltr_TrmY |
| Scope(s) |
Bacteria Gammaproteobacteria Archaea |
| Template(s) | Q59034; D4GTL8; [ Recover all ] |
| Triggered by |
HAMAP; MF_00587 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| case <OC:Archaea> | |
| Identifier | TRMY |
| Protein name | RecName: Full=tRNA (pseudouridine(54)-N(1))-methyltransferase; EC=2.1.1.257; |
| Gene name | Name=trmY; |
| else case <OC:Bacteria> | |
| Identifier | TRMYL |
| Protein name | RecName: Full=Putative pseudouridine methyltransferase; EC=2.1.1.-; |
| end case | |
Comments
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| case <OC:Archaea> | |
| FUNCTION | Specifically catalyzes the N1-methylation of pseudouridine at position 54 (Psi54) in tRNAs. |
| CATALYTIC ACTIVITY | Reaction=pseudouridine(54) in tRNA + S-adenosyl-L-methionine = N(1)- methylpseudouridine(54) in tRNA + S-adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:55292, Rhea:RHEA-COMP:14140, Rhea:RHEA-COMP:14141, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:65314, ChEBI:CHEBI:74890; EC=2.1.1.257; |
| SUBUNIT | Homodimer. |
| end case | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the methyltransferase superfamily. TrmY family. |
Keywords
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| Cytoplasm | |
| Methyltransferase | |
| S-adenosyl-L-methionine | |
| Transferase | |
| case <OC:Archaea> | |
| tRNA processing | |
| end case | |
Gene Ontology
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| case <OCellular component:Archaea> | |
| GO:0030488; Biological process:tRNA methylation | |
| GO:0008175; Molecular function:tRNA methyltransferase activity | |
| end case | |
| GO:0008757; Molecular function:S-adenosylmethionine-dependent methyltransferase activity | |
| GO:0005737; Cellular component:cytoplasm | |
Cross-references
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| Pfam | PF04013; Methyltrn_RNA_2; 1; |
Features
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| From: TRMY_METJA (Q59034) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 179 | 184 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[LIV]-S-P-L-[ES]-L | ||||||||
| BINDING | 136 | 136 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[LM] | ||||||||
| BINDING | 156 | 156 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
G | ||||||||
| BINDING | 189 | 189 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
C | ||||||||
Additional information
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| Size range | 192-211 amino acids |
| Related rules |
None |
| Fusion | Nter: <Unknown> Cter: None |
| Comments | Formation of 1-methylpseudouridine at position 54 of tRNA has never been identified in any bacterial tRNA sequenced so far. The bacterial and archaeal homologs may fulfill different functions. See PubMed:22274953. Unknown N-terminal domain in ARCFU. Divergent PYRAE not shown in alignment and not used in size range. |