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HAMAP rule MF_00587

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General rule information [?]

Accession MF_00587
Dates 22-OCT-2002 (Created)
19-NOV-2022 (Last updated, Version 28)
Name tRNA_methyltr_TrmY
Scope(s) Bacteria
Template(s) Q59034 (TRMY_METJA); D4GTL8 (TRMY_HALVD); [ Recover all ]
Triggered by HAMAP; MF_00587 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Archaea>
Identifier TRMY
Protein name RecName: Full=tRNA (pseudouridine(54)-N(1))-methyltransferase;
Gene name Name=trmY;
else case <OC:Bacteria>
Identifier TRMYL
Protein name RecName: Full=Putative pseudouridine methyltransferase;
end case

Comments [?]

case <OC:Archaea>
FUNCTIONSpecifically catalyzes the N1-methylation of pseudouridine at position 54 (Psi54) in tRNAs.
CATALYTIC ACTIVITY Reaction=pseudouridine(54) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylpseudouridine(54) in tRNA + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:55292, Rhea:RHEA-COMP:14140, Rhea:RHEA-COMP:14141, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:65314, ChEBI:CHEBI:74890; EC=;
end case
SIMILARITYBelongs to the methyltransferase superfamily. TrmY family.

Keywords [?]

Gene Ontology [?]

case <OCellular component:Archaea>
GO:0030488; Biological process:tRNA methylation
GO:0008175; Molecular function:tRNA methyltransferase activity
end case
GO:0008757; Molecular function:S-adenosylmethionine-dependent methyltransferase activity
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF04013; Methyltrn_RNA_2; 1;

Features [?]

From: TRMY_METJA (Q59034)
Key From To Description Tag Condition FTGroup
BINDING 179 184 /ligand="S-adenosyl-L-methionine"
BINDING 136 136 /ligand="S-adenosyl-L-methionine"
BINDING 156 156 /ligand="S-adenosyl-L-methionine"
BINDING 189 189 /ligand="S-adenosyl-L-methionine"

Additional information [?]

Size range 192-211 amino acids
Related rules None
Fusion Nter: <Unknown> Cter: None
Comments Formation of 1-methylpseudouridine at position 54 of tRNA has never been identified in any bacterial tRNA sequenced so far. The bacterial and archaeal homologs may fulfill different functions. See PubMed:22274953. Unknown N-terminal domain in ARCFU. Divergent PYRAE not shown in alignment and not used in size range.

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