HAMAP rule MF_00593
General rule information
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| Accession | MF_00593 |
| Dates | 1-NOV-2002 (Created)
1-JUN-2023 (Last updated, Version 33) |
| Name | DltA |
| Scope(s) |
Bacteria |
| Template(s) | P39581 (DLTA_BACSU); Q81G39 (DLTA_BACCR); Q99ZA6 (DLTA_STRP1); [ Recover all ] |
| Triggered by |
HAMAP; MF_00593 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | DLTA |
| Protein name | RecName: Full=D-alanine--D-alanyl carrier protein ligase; Short=DCL; EC=6.2.1.54; AltName: Full=D-alanine--poly(phosphoribitol) ligase subunit 1; AltName: Full=D-alanine-activating enzyme; Short=DAE; |
| Gene name | Name=dltA; |
Comments
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| FUNCTION | Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D- alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall. |
| CATALYTIC ACTIVITY | Reaction=ATP + D-alanine + holo-[D-alanyl-carrier protein] = AMP + D- alanyl-[D-alanyl-carrier protein] + diphosphate; Xref=Rhea:RHEA:55132, Rhea:RHEA-COMP:14102, Rhea:RHEA-COMP:14103, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57416, ChEBI:CHEBI:64479, ChEBI:CHEBI:138620, ChEBI:CHEBI:456215; EC=6.2.1.54; |
| PATHWAY | Cell wall biogenesis; lipoteichoic acid biosynthesis. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the ATP-dependent AMP-binding enzyme family. DltA subfamily. |
Keywords
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Gene Ontology
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| GO:0047473; Molecular function:D-alanine [D-alanyl carrier protein] ligase activity |
| GO:0005737; Cellular component:cytoplasm |
| GO:0070395; Biological process:lipoteichoic acid biosynthetic process |
Cross-references
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| Pfam | PF00501; AMP-binding; 1; |
| Pfam | PF13193; AMP-binding_C; 1; |
| NCBIfam | TIGR01733; AA-adenyl-dom; 1; |
| NCBIfam | TIGR01734; D-ala-DACP-lig; 1; |
| PROSITE | PS00455; AMP_BINDING; 1; |
Features
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| From: DLTA_BACCR (Q81G39) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 152 | 153 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
T-S | ||||||||
| BINDING | 292 | 297 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
N-[TA]-Y-G-P-T | ||||||||
| BINDING | 394 | 397 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
Y-x-G-R | ||||||||
| BINDING | 197 | 197 | /ligand="D-alanine" /ligand_id="ChEBI:CHEBI:57416" |
D | ||||||||
| BINDING | 301 | 301 | /ligand="D-alanine" /ligand_id="ChEBI:CHEBI:57416" |
V | ||||||||
| BINDING | 383 | 383 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
D | ||||||||
| BINDING | 492 | 492 | /ligand="D-alanine" /ligand_id="ChEBI:CHEBI:57416" |
K | ||||||||
| BINDING | 492 | 492 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K | ||||||||
Additional information
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| Size range | 485-516 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |