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HAMAP rule MF_00593

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_00593
Accession MF_00593
Dates 28-FEB-2005 (Created)
03-SEP-2024 (Last updated, Version 26)
Name DltA
Scope(s) Bacteria
Template(s) P39581; Q81G39; Q99ZA6; [ Recover all ]
Triggered by HAMAP; MF_00593 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier DLTA
Protein name RecName: Full=D-alanine--D-alanyl carrier protein ligase;
                 Short=DCL;
                 EC=6.2.1.54;
AltName: Full=D-alanine--poly(phosphoribitol) ligase subunit 1;
AltName: Full=D-alanine-activating enzyme;
                 Short=DAE;
Gene name Name=dltA;

Comments [?]

FUNCTIONCatalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D- alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.
CATALYTIC ACTIVITY Reaction=holo-[D-alanyl-carrier protein] + D-alanine + ATP = D-alanyl- [D-alanyl-carrier protein] + AMP + diphosphate; Xref=Rhea:RHEA:55132, Rhea:RHEA-COMP:14102, Rhea:RHEA-COMP:14103, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57416, ChEBI:CHEBI:64479, ChEBI:CHEBI:138620, ChEBI:CHEBI:456215; EC=6.2.1.54;
PATHWAYCell wall biogenesis; lipoteichoic acid biosynthesis.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the ATP-dependent AMP-binding enzyme family. DltA subfamily.

Keywords [?]

ATP-binding
Cytoplasm
Ligase
Nucleotide-binding

Gene Ontology [?]

GO:0047473; Molecular function:D-alanine [D-alanyl carrier protein] ligase activity
GO:0005737; Cellular component:cytoplasm
GO:0070395; Biological process:lipoteichoic acid biosynthetic process

Cross-references [?]

Pfam PF00501; AMP-binding; 1;
Pfam PF13193; AMP-binding_C; 1;
NCBIfam TIGR01733; AA-adenyl-dom; 1;
NCBIfam TIGR01734; D-ala-DACP-lig; 1;
PROSITE PS00455; AMP_BINDING; 1;

Features [?]

From: DLTA_BACCR (Q81G39)
Key From To Description Tag Condition FTGroup
BINDING 152 153 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 T-S
BINDING 292 297 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 N-[TA]-Y-G-P-T
BINDING 394 397 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 Y-x-G-R
BINDING 197 197 /ligand="D-alanine"
/ligand_id="ChEBI:CHEBI:57416"		 D
BINDING 301 301 /ligand="D-alanine"
/ligand_id="ChEBI:CHEBI:57416"		 V
BINDING 383 383 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 D
BINDING 492 492 /ligand="D-alanine"
/ligand_id="ChEBI:CHEBI:57416"		 K
BINDING 492 492 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 K

Additional information [?]

Size range 485-516 amino acids
Related rules None
Fusion Nter: None Cter: None



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