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Annotation rule MF_00593
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General rule information [?]

Accession MF_00593
Dates 1-NOV-2002 (Created)
18-NOV-2019 (Last updated, Version 30)
Name DltA
Scope
Bacteria
Templates P39581 (DLTA_BACSU); Q81G39 (DLTA_BACCR); Q99ZA6 (DLTA_STRP1): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
DLTA
Protein name
RecName: Full=D-alanine--D-alanyl carrier protein ligase;
Short=DCL;
EC 6.2.1.54;
AltName: Full=D-alanine--poly(phosphoribitol) ligase subunit 1;
AltName: Full=D-alanine-activating enzyme;
Short=DAE;
Gene name
dltA

Comments [?]

Function Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.
Catalytic activity RHEA:55132: ATP + D-alanine + holo-[D-alanyl-carrier protein] = AMP + D-alanyl-[D-alanyl-carrier protein] + diphosphate
EC 6.2.1.54
Pathway Cell wall biogenesis; lipoteichoic acid biosynthesis.
Subcellular location Cytoplasm.
Similarity Belongs to the ATP-dependent AMP-binding enzyme family. DltA subfamily.

Keywords [?]


Gene Ontology [?]

GO:0047473; Molecular function: D-alanine [D-alanyl carrier protein] ligase activity.
GO:0005737; Cellular component: cytoplasm.
GO:0070395; Biological process: lipoteichoic acid biosynthetic process.

Cross-references [?]

Pfam PF00501; AMP-binding; 1;
PF13193; AMP-binding_C; 1;
TIGRFAMs TIGR01733; AA-adenyl-dom; 1;
TIGR01734; D-ala-DACP-lig; 1;
PROSITE PS00455; AMP_BINDING; 1;

Features [?]

From: DLTA_BACCR (Q81G39)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     152     153       ATP     T-S  
NP_BIND     292     297       ATP     N-[TA]-Y-G-P-T  
NP_BIND (Optional)     394     397       ATP     Y-x-G-R  
BINDING     197     197       D-alanine     D  
BINDING     301     301       D-alanine; via carbonyl oxygen     V  
BINDING     383     383       ATP     D  
BINDING     492     492       D-alanine     K  
BINDING     492     492       ATP     K  

Additional information [?]

Size range 485-516 amino acids
Related rules None
Fusion None