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Annotation rule MF_00627
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General rule information [?]

Accession MF_00627
Dates 24-MAR-2003 (Created)
18-NOV-2019 (Last updated, Version 26)
Name Thr_dehydrog
Scope
Bacteria
Archaea
Templates P07913 (TDH_ECOLI); O58389 (TDH_PYRHO); Q5JI69 (TDH_THEKO); Q8U259 (TDH_PYRFU); Q5SKS4 (TDH_THET8): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
TDH
Protein name
RecName: Full=L-threonine 3-dehydrogenase;
Short=TDH;
EC 1.1.1.103;
Gene name
tdh

Comments [?]

Function Catalyzes the NAD(+)-dependent oxidation of L-threonine to 2-amino-3-ketobutyrate.
Catalytic activity RHEA:13161: L-threonine + NAD(+) = (2S)-2-amino-3-oxobutanoate + H(+) + NADH
EC 1.1.1.103
case <FTGroup:1> and <FTGroup:2>
Cofactor Zn(2+)
Note: Binds 2 Zn(2+) ions per subunit.
end case
case <FTGroup:1> and not <FTGroup:2>
Cofactor Zn(2+)
Note: Binds 1 Zn(2+) ion per subunit.
end case
Pathway Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2.
Subunit Homotetramer.
Subcellular location Cytoplasm.
Similarity Belongs to the zinc-containing alcohol dehydrogenase family.

Keywords [?]

case <FTGroup:1> or <FTGroup:2>
end case
NAD

Gene Ontology [?]

GO:0008743; Molecular function: L-threonine 3-dehydrogenase activity.
case <FTGroup:1> or <FTGroup:2>
GO:0008270; Molecular function: zinc ion binding.
end case
GO:0006567; Biological process: threonine catabolic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF08240; ADH_N; 1;
PF00107; ADH_zinc_N; 1;
TIGRFAMs TIGR00692; tdh; 1;
PROSITE PS00059; ADH_ZINC; 1;

Features [?]

From: TDH_PYRHO (O58389)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     266     268       NAD     L-x-[LIT]  
NP_BIND     291     292       NAD     [IV]-[TY]  
ACT_SITE     44     44       Charge relay system     T  
ACT_SITE     47     47       Charge relay system     H  
METAL     42     42       Zinc 1; catalytic     C   1
METAL     67     67       Zinc 1; via tele nitrogen; catalytic     H   1
METAL     68     68       Zinc 1; catalytic     E   1
METAL     97     97       Zinc 2     C   2
METAL     100     100       Zinc 2     C   2
METAL     103     103       Zinc 2     C   2
METAL     111     111       Zinc 2     C   2
BINDING     179     179       NAD; via amide nitrogen     [LIV]  
BINDING     199     199       NAD     [ED]  
BINDING     204     204       NAD     R  
SITE     152     152       Important for catalytic activity for the proton relay mechanism but does not participate directly in the coordination of zinc atom     [DE]  

Additional information [?]

Size range 340-361 amino acids
Related rules None
Fusion None