HAMAP rule MF_00627
General rule information
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| Accession | MF_00627 |
| Dates | 24-MAR-2003 (Created)
2-SEP-2024 (Last updated, Version 30) |
| Name | Thr_dehydrog |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P07913 (TDH_ECOLI); O58389 (TDH_PYRHO); Q5JI69 (TDH_THEKO); Q8U259 (TDH_PYRFU); Q5SKS4 (TDH_THET8); [ Recover all ] |
| Triggered by |
HAMAP; MF_00627 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | TDH |
| Protein name | RecName: Full=L-threonine 3-dehydrogenase; Short=TDH; EC=1.1.1.103; |
| Gene name | Name=tdh; |
Comments
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| FUNCTION | Catalyzes the NAD(+)-dependent oxidation of L-threonine to 2- amino-3-ketobutyrate. |
| CATALYTIC ACTIVITY | Reaction=L-threonine + NAD(+) = (2S)-2-amino-3-oxobutanoate + NADH + H(+); Xref=Rhea:RHEA:13161, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57926, ChEBI:CHEBI:57945, ChEBI:CHEBI:78948; EC=1.1.1.103; |
| case <FTGroup:1> and <FTGroup:2> | |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 2 Zn(2+) ions per subunit.; |
| end case | |
| case <FTGroup:1> and not <FTGroup:2> | |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 Zn(2+) ion per subunit.; |
| end case | |
| PATHWAY | Amino-acid degradation; L-threonine degradation via oxydo- reductase pathway; glycine from L-threonine: step 1/2. |
| SUBUNIT | Homotetramer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the zinc-containing alcohol dehydrogenase family. |
Keywords
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| Cytoplasm | |
| Oxidoreductase | |
| case <FTGroup:1> or <FTGroup:2> | |
| Zinc | |
| Metal-binding | |
| end case | |
| NAD | |
Gene Ontology
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| GO:0008743; Molecular function:L-threonine 3-dehydrogenase activity | |
| case <FTGroup:1> or <FTGroup:2> | |
| GO:0008270; Molecular function:zinc ion binding | |
| end case | |
| GO:0006567; Biological process:threonine catabolic process | |
| GO:0005737; Cellular component:cytoplasm | |
Cross-references
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| Pfam | PF08240; ADH_N; 1; |
| Pfam | PF00107; ADH_zinc_N; 1; |
| NCBIfam | TIGR00692; tdh; 1; |
| PROSITE | PS00059; ADH_ZINC; 1; |
Features
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| From: TDH_PYRHO (O58389) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 266 | 268 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
L-x-[LIT] | ||||||||
| BINDING | 291 | 292 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
[IV]-[TY] | ||||||||
| ACT_SITE | 44 | 44 | /note="Charge relay system" | T | ||||||||
| ACT_SITE | 47 | 47 | /note="Charge relay system" | H | ||||||||
| BINDING | 42 | 42 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" /ligand_note="catalytic" |
C | 1 | |||||||
| BINDING | 67 | 67 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" /ligand_note="catalytic" |
H | 1 | |||||||
| BINDING | 68 | 68 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" /ligand_note="catalytic" |
E | 1 | |||||||
| BINDING | 97 | 97 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" |
C | 2 | |||||||
| BINDING | 100 | 100 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" |
C | 2 | |||||||
| BINDING | 103 | 103 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" |
C | 2 | |||||||
| BINDING | 111 | 111 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" |
C | 2 | |||||||
| BINDING | 179 | 179 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
[LIV] | ||||||||
| BINDING | 199 | 199 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
[ED] | ||||||||
| BINDING | 204 | 204 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
R | ||||||||
| SITE | 152 | 152 | /note="Important for catalytic activity for the proton relay mechanism but does not participate directly in the coordination of zinc atom" | [DE] | ||||||||
Additional information
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| Size range | 340-361 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |