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Annotation rule MF_00664 |
Accession | MF_00664 |
Dates | 28-JUN-2003 (Created) 18-MAR-2020 (Last updated, Version 31) |
Name | PS_decarb_PSD_A |
Scope | Bacteria
Archaea |
Templates | Q9FDI9 (PSD_RHIME); O28234 (ASD_ARCFU); P0A8K1 (PSD_ECOLI): [Recover all] |
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Function | Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). |
Catalytic activity | RHEA:20828: a 1,2-diacyl-sn-glycero-3-phospho-L-serine + H(+) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CO2
EC 4.1.1.65 |
Pathway | Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 2/2. |
Function | Catalyzes the formation of archaetidylethanolamine (PtdEtn) from archaetidylserine (PtdSer). |
Catalytic activity | RHEA:51488: archaetidylserine + H(+) = archaetidylethanolamine + CO2 |
Cofactor | pyruvate Note: Binds 1 pyruvoyl group covalently per subunit. |
Subunit | Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit. |
Subcellular location | Cell membrane; Peripheral membrane protein. |
Ptm | Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain. |
Similarity | Belongs to the phosphatidylserine decarboxylase family. PSD-A subfamily. |
From: PSD_RHIME (Q9FDI9) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
CHAIN | Nter | 189 | Phosphatidylserine decarboxylase beta chain | |||||||||
CHAIN | 190 | Cter | Phosphatidylserine decarboxylase alpha chain |
CHAIN | Nter | 189 | Archaetidylserine decarboxylase beta chain | |||||||||
CHAIN | 190 | Cter | Archaetidylserine decarboxylase alpha chain |
ACT_SITE | 190 | 190 | Schiff-base intermediate with substrate; via pyruvic acid | S | ||||||||
SITE | 189 | 190 | Cleavage (non-hydrolytic); by autocatalysis | G-S | ||||||||
MOD_RES | 190 | 190 | Pyruvic acid (Ser); by autocatalysis | S |
Size range | 195-265 amino acids |
Related rules | MF_00662 (PSD); MF_00663 (PSD) |
Fusion | None |
Comments | Classification of phosphatidylserine decarboxylase into subfamilies was done according to Daiyasu at al.(2005) (PubMed:16243780). |