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HAMAP rule MF_00772

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General rule information [?]

Accession MF_00772
Dates 16-APR-2004 (Created)
1-JUN-2023 (Last updated, Version 22)
Name OGT
Scope(s) Archaea
Template(s) O74023 (OGT_THEKO); Q58924 (OGT_METJA); P11742 (OGT_BACSU); [ Recover all ]
Triggered by HAMAP; MF_00772 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier OGT
Protein name RecName: Full=Methylated-DNA--protein-cysteine methyltransferase;
AltName: Full=6-O-methylguanine-DNA methyltransferase;
AltName: Full=O-6-methylguanine-DNA-alkyltransferase;
case <OC:Archaea>
Gene name Name=ogt;
end case

Comments [?]

FUNCTIONInvolved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
CATALYTIC ACTIVITY Reaction=a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:24000, Rhea:RHEA-COMP:10131, Rhea:RHEA-COMP:10132, Rhea:RHEA-COMP:11367, Rhea:RHEA-COMP:11368, ChEBI:CHEBI:29950, ChEBI:CHEBI:82612, ChEBI:CHEBI:85445, ChEBI:CHEBI:85448; EC=;
CATALYTIC ACTIVITY Reaction=a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:53428, Rhea:RHEA-COMP:10131, Rhea:RHEA-COMP:10132, Rhea:RHEA-COMP:13555, Rhea:RHEA-COMP:13556, ChEBI:CHEBI:29950, ChEBI:CHEBI:82612, ChEBI:CHEBI:137386, ChEBI:CHEBI:137387; EC=;
MISCELLANEOUSThis enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.
SIMILARITYBelongs to the MGMT family.

Keywords [?]

Gene Ontology [?]

GO:0003908; Molecular function:methylated-DNA-[protein]-cysteine S-methyltransferase activity
GO:0006307; Biological process:DNA dealkylation involved in DNA repair
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF01035; DNA_binding_1; 1;
NCBIfam TIGR00589; Ogt; 1;

Features [?]

From: OGT_THEKO (O74023)
Key From To Description Tag Condition FTGroup
ACT_SITE 141 141 /note="Nucleophile; methyl group acceptor" C

Additional information [?]

Size range 131-220 amino acids
Related rules None
Fusion Nter: None Cter: None

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