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Annotation rule MF_00818 |
Accession | MF_00818 |
Dates | 8-NOV-2005 (Created) 18-NOV-2019 (Last updated, Version 20) |
Name | QueF_type1 |
Scope | Bacteria |
Templates | O31678 (QUEF_BACSU); Q5L1B7 (QUEF_GEOKA): [Recover all] |
Triggered by |
Identifier |
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Protein name |
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Gene name |
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Function | Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). |
Catalytic activity | RHEA:13409: 7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-deazaguanine + 3 H(+) + 2 NADPH
EC 1.7.1.13 |
Pathway | tRNA modification; tRNA-queuosine biosynthesis. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily. |
Pfam | PF01227; GTP_cyclohydroI; 1; |
PIRSF | PIRSF027377; Nitrile_oxidored_QueF; 1; |
TIGRFAMs | TIGR03139; QueF-II; 1; |
From: QUEF_BACSU (O31678) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | 78 | 80 | Substrate binding | [VMILY]-E-[STL] | ||||||||
REGION | 97 | 98 | Substrate binding | [HYMQ]-E | ||||||||
ACT_SITE | 56 | 56 | Thioimide intermediate | C | ||||||||
ACT_SITE | 63 | 63 | Proton donor | D |
Size range | 116-174 amino acids |
Related rules | MF_00817 (QUEF) |
Fusion | None |