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HAMAP rule MF_00818

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General rule information [?]

Accession MF_00818
Dates 8-NOV-2005 (Created)
1-JUN-2023 (Last updated, Version 23)
Name QueF_type1
Scope(s) Bacteria
Template(s) O31678 (QUEF_BACSU); Q5L1B7 (QUEF_GEOKA); [ Recover all ]
Triggered by HAMAP; MF_00818 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier QUEF
Protein name RecName: Full=NADPH-dependent 7-cyano-7-deazaguanine reductase;
                 EC=1.7.1.13;
AltName: Full=7-cyano-7-carbaguanine reductase;
AltName: Full=NADPH-dependent nitrile oxidoreductase;
AltName: Full=PreQ(0) reductase;
Gene name Name=queF;

Comments [?]

FUNCTIONCatalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).
CATALYTIC ACTIVITY Reaction=7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7- deazaguanine + 3 H(+) + 2 NADPH; Xref=Rhea:RHEA:13409, ChEBI:CHEBI:15378, ChEBI:CHEBI:45075, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:58703; EC=1.7.1.13;
PATHWAYtRNA modification; tRNA-queuosine biosynthesis.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Keywords [?]


Gene Ontology [?]

GO:0033739; Molecular function:preQ1 synthase activity
GO:0008616; Biological process:queuosine biosynthetic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF01227; GTP_cyclohydroI; 1;
PIRSF PIRSF027377; Nitrile_oxidored_QueF; 1;
NCBIfam TIGR03139; QueF-II; 1;

Features [?]

From: QUEF_BACSU (O31678)
Key From To Description Tag Condition FTGroup
BINDING 78 80 /ligand="substrate" [VMILY]-E-[STL]
BINDING 97 98 /ligand="substrate" [HYMQ]-E
ACT_SITE 56 56 /note="Thioimide intermediate" C
ACT_SITE 63 63 /note="Proton donor" D

Additional information [?]

Size range 116-174 amino acids
Related rules MF_00817
Fusion Nter: None Cter: None



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