HAMAP rule MF_00818

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_00818
Accession	MF_00818
Dates	08-NOV-2005 (Created) 12-JUN-2025 (Last updated, Version 28)
Name	QueF_type1
Scope(s)	Bacteria
Template(s)	O31678; Q5L1B7; [ Recover all ]
Triggered by	HAMAP; MF_00818 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	QUEF
Protein name	RecName: Full=NADPH-dependent 7-cyano-7-deazaguanine reductase; EC=1.7.1.13; AltName: Full=7-cyano-7-carbaguanine reductase; AltName: Full=NADPH-dependent nitrile oxidoreductase; AltName: Full=PreQ(0) reductase;
Gene name	Name=queF;

Comments [?]

FUNCTION	Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).
CATALYTIC ACTIVITY	Reaction=7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7- carbaguanine + 2 NADPH + 3 H(+); Xref=Rhea:RHEA:13409, ChEBI:CHEBI:15378, ChEBI:CHEBI:45075, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:58703; EC=1.7.1.13;
PATHWAY	tRNA modification; tRNA-queuosine biosynthesis.
SUBCELLULAR LOCATION	Cytoplasm.
SIMILARITY	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0033739; Molecular function:preQ1 synthase activity

GO:0008616; Biological process:tRNA queuosine(34) biosynthetic process

GO:0006400; Biological process:tRNA modification

GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Features [?]

From: QUEF_BACSU (O31678)

Key

From

Description

Tag

Condition

FTGroup

BINDING

/ligand="substrate"

[VMILY]-E-[STL]

BINDING

/ligand="substrate"

[HYMQ]-E

ACT_SITE

/note="Thioimide intermediate"

ACT_SITE

/note="Proton donor"

Additional information [?]