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Annotation rule MF_00818
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General rule information [?]

Accession MF_00818
Dates 8-NOV-2005 (Created)
18-NOV-2019 (Last updated, Version 20)
Name QueF_type1
Templates O31678 (QUEF_BACSU); Q5L1B7 (QUEF_GEOKA): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=NADPH-dependent 7-cyano-7-deazaguanine reductase;
AltName: Full=7-cyano-7-carbaguanine reductase;
AltName: Full=NADPH-dependent nitrile oxidoreductase;
AltName: Full=PreQ(0) reductase;
Gene name

Comments [?]

Function Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).
Catalytic activity RHEA:13409: 7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-deazaguanine + 3 H(+) + 2 NADPH
Pathway tRNA modification; tRNA-queuosine biosynthesis.
Subcellular location Cytoplasm.
Similarity Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0033739; Molecular function: preQ1 synthase activity.
GO:0008616; Biological process: queuosine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF01227; GTP_cyclohydroI; 1;
PIRSF PIRSF027377; Nitrile_oxidored_QueF; 1;
TIGRFAMs TIGR03139; QueF-II; 1;

Features [?]

From: QUEF_BACSU (O31678)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     78     80       Substrate binding     [VMILY]-E-[STL]  
REGION     97     98       Substrate binding     [HYMQ]-E  
ACT_SITE     56     56       Thioimide intermediate     C  
ACT_SITE     63     63       Proton donor     D  

Additional information [?]

Size range 116-174 amino acids
Related rules MF_00817 (QUEF)
Fusion None