HAMAP rule MF_00825

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_00825
Accession	MF_00825
Dates	21-JUN-2006 (Created) 13-JUN-2025 (Last updated, Version 26)
Name	3_HAO
Scope(s)	Bacteria
Template(s)	Q1LCS4; Q83V26; [ Recover all ]
Triggered by	case c? <OC:Bacteria> HAMAP; MF_00825 (Get profile general information and statistics) end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	3HAO
Protein name	RecName: Full=3-hydroxyanthranilate 3,4-dioxygenase; EC=1.13.11.6; AltName: Full=3-hydroxyanthranilate oxygenase; Short=3-HAO; AltName: Full=3-hydroxyanthranilic acid dioxygenase; Short=HAD;
Gene name	Name=nbaC;

Comments [?]

FUNCTION	Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.
CATALYTIC ACTIVITY	Reaction=3-hydroxyanthranilate + O2 = (2Z,4Z)-2-amino-3-carboxymuconate 6-semialdehyde; Xref=Rhea:RHEA:17953, ChEBI:CHEBI:15379, ChEBI:CHEBI:36559, ChEBI:CHEBI:77612; EC=1.13.11.6;
case <FTGroup:1> and <FTGroup:2>
COFACTOR	Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Note=Binds 2 Fe(2+) ions per subunit.;
else case <FTGroup:1> or <FTGroup:2>
COFACTOR	Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
end case
PATHWAY	Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 3/3.
case <OC:Pseudomonadota>
SUBUNIT	Homodimer.
end case
SIMILARITY	Belongs to the 3-HAO family.

Keywords [?]

Dioxygenase
Oxidoreductase
Pyridine nucleotide biosynthesis
case <FTGroup:1> or <FTGroup:2>
Iron
Metal-binding
end case

Gene Ontology [?]

GO:0000334; Molecular function:3-hydroxyanthranilate 3,4-dioxygenase activity

GO:0008198; Molecular function:ferrous iron binding

GO:0006569; Biological process:L-tryptophan catabolic process

GO:0019805; Biological process:quinolinate biosynthetic process

GO:0034354; Biological process:'de novo' NAD+ biosynthetic process from L-tryptophan

GO:0043420; Biological process:anthranilate metabolic process

Cross-references [?]

Pfam	PF06052; 3-HAO; 1;
NCBIfam	TIGR03037; Anthran_nbaC; 1;

Features [?]

From: 3HAO_CUPMC (Q1LCS4)
Key	From	To	Description	Tag	Condition	FTGroup
BINDING	51	51	/ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="1" /ligand_note="catalytic"		H	1
BINDING	57	57	/ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="1" /ligand_note="catalytic"		E	1
BINDING	95	95	/ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="1" /ligand_note="catalytic"		H	1
BINDING	125	125	/ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="2"		C	2
BINDING	128	128	/ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="2"		C	2
BINDING	162	162	/ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="2"		C	2
BINDING	165	165	/ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="2"		C	2
BINDING	47	47	/ligand="O2" /ligand_id="ChEBI:CHEBI:15379"		R
BINDING	57	57	/ligand="substrate"		E
BINDING	99	99	/ligand="substrate"		R
BINDING	110	110	/ligand="substrate"		E

Additional information [?]